Table 1.
Gene symbol | Oligo | Sequence | Amplicon size (bp) |
---|---|---|---|
ACTB | Forward | CAGCCATGTACGTTGCTATCCA | 82 |
Reverse | TCACCGGAGTCCATCACGAT | ||
TaqMan® probe | TGTGCTATCCCTGTACGCCTCTGGCC | ||
LINE1 | Forward | TTGCGGCATTATTCACAATAGC | 83 |
Reverse | GTGCCACATTTTCTTAATCCAGTCT | ||
TaqMan probe | TGGAACCAACCCAAATGTCCAACAATGA | ||
TOP1 | Forward | GGCGAGTGAATCTAAGGATAATGAA | 97 |
Reverse | TGGATATCTTAAAGGGTACAGCGAA | ||
TaqMan probe | TCAGTGACTGAAACCATTTTCCCATCATCC | ||
TYMS | Forward | GCCTCGGTGTGCCTTTCA | 65 |
Reverse | CGTGATGTGCGCAATCATG | ||
TaqMan probe | CATCGCCAGCTACGCCCTGCTC | ||
EGFR | Forward | GAATTCGGATGCAGAGCTTC | 150 |
Reverse | GACATGCTGCGGTGTTTTC | ||
TaqMan probe | TCCCATGATGATCTGTCCCTCACA |
qPCR: Qualitative PCR.