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. 1992 Feb;60(2):345–352. doi: 10.1128/iai.60.2.345-352.1992

Characterization of inhibition of Mycobacterium avium replication in macrophages by normal human serum.

G S Douvas 1, M H May 1, E Ross 1, A J Crowle 1
PMCID: PMC257634  PMID: 1730466

Abstract

Serum from some AIDS patients permits the rapid multiplication of Mycobacterium avium in cultured human macrophages. Serum from human immunodeficiency virus-negative individuals inhibits replication. The characteristics of the serum-induced inhibition were examined here. M. avium 7497 serovar 4 grew exponentially in macrophages when they were cultured in serumless medium. Growth was measured by determining the CFU after infected macrophages were lysed at 0 to 7 days after infection. Normal AB serum (5 to 10%) added to infected macrophages resulted in an initial 4-day lag of bacterial growth followed by rapid replication from 4 to 7 days. Serum also inhibited bacterial replication in medium without macrophages. This inhibition was not biphasic but was sustained over 7 days. Macrophage-associated M. avium became less responsive to serum inhibitor within 24 h after infection of macrophages. Within 2 days of culture, M. avium no longer responded to inhibitor. Replication of macrophage-derived M. avium (Vi) was in some instances serum inhibitable and at other times was enhanced by serum, when it was used to infect fresh macrophages. The Vi phenotype remained serum inhibitable without macrophages. Preinfection of macrophages with heat-killed M. avium did not alter serum-induced bacterial inhibition or escape from inhibition.

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Selected References

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  1. Barclay R., Ratledge C. Participation of iron on the growth inhibition of pathogenic strains of mycobacterium avium and M. paratuberculosis in serum. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Aug;262(2):189–194. doi: 10.1016/s0176-6724(86)80019-0. [DOI] [PubMed] [Google Scholar]
  2. Blanchard D. K., Michelini-Norris M. B., Pearson C. A., Freitag C. S., Djeu J. Y. Mycobacterium avium-intracellulare induces interleukin-6 from human monocytes and large granular lymphocytes. Blood. 1991 May 15;77(10):2218–2224. [PubMed] [Google Scholar]
  3. Crowle A. J., Cohn D. L., Poche P. Defects in sera from acquired immunodeficiency syndrome (AIDS) patients and from non-AIDS patients with Mycobacterium avium infection which decrease macrophage resistance to M. avium. Infect Immun. 1989 May;57(5):1445–1451. doi: 10.1128/iai.57.5.1445-1451.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crowle A. J., Dahl R., Ross E., May M. H. Evidence that vesicles containing living, virulent Mycobacterium tuberculosis or Mycobacterium avium in cultured human macrophages are not acidic. Infect Immun. 1991 May;59(5):1823–1831. doi: 10.1128/iai.59.5.1823-1831.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crowle A. J., Poche P. Inhibition by normal human serum of Mycobacterium avium multiplication in cultured human macrophages. Infect Immun. 1989 Apr;57(4):1332–1335. doi: 10.1128/iai.57.4.1332-1335.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crowle A. J., Sbarbaro J. A., Judson F. N., Douvas G. S., May M. H. Inhibition by streptomycin of tubercle bacilli within cultured human macrophages. Am Rev Respir Dis. 1984 Nov;130(5):839–844. doi: 10.1164/arrd.1984.130.5.839. [DOI] [PubMed] [Google Scholar]
  7. Crowle A. J., Tsang A. Y., Vatter A. E., May M. H. Comparison of 15 laboratory and patient-derived strains of Mycobacterium avium for ability to infect and multiply in cultured human macrophages. J Clin Microbiol. 1986 Nov;24(5):812–821. doi: 10.1128/jcm.24.5.812-821.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. David H. L. Basis for lack of drug susceptibility of atypical mycobacteria. Rev Infect Dis. 1981 Sep-Oct;3(5):878–884. doi: 10.1093/clinids/3.5.878. [DOI] [PubMed] [Google Scholar]
  9. Denis M., Gregg E. O. Recombinant tumour necrosis factor-alpha decreases whereas recombinant interleukin-6 increases growth of a virulent strain of Mycobacterium avium in human macrophages. Immunology. 1990 Sep;71(1):139–141. [PMC free article] [PubMed] [Google Scholar]
  10. Douvas G. S., Berger E. M., Repine J. E., Crowle A. J. Natural mycobacteriostatic activity in human monocyte-derived adherent cells. Am Rev Respir Dis. 1986 Jul;134(1):44–48. doi: 10.1164/arrd.1986.134.1.44. [DOI] [PubMed] [Google Scholar]
  11. Douvas G. S., Looker D. L., Vatter A. E., Crowle A. J. Gamma interferon activates human macrophages to become tumoricidal and leishmanicidal but enhances replication of macrophage-associated mycobacteria. Infect Immun. 1985 Oct;50(1):1–8. doi: 10.1128/iai.50.1.1-8.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ellner J. J. Sources of variability in assays of the interaction of mycobacteria with mononuclear phagocytes: of mice and men. Res Microbiol. 1990 Feb;141(2):237–240. doi: 10.1016/0923-2508(90)90036-p. [DOI] [PubMed] [Google Scholar]
  13. Frehel C., Ryter A., Rastogi N., David H. The electron-transparent zone in phagocytized Mycobacterium avium and other mycobacteria: formation, persistence and role in bacterial survival. Ann Inst Pasteur Microbiol. 1986 Nov-Dec;137B(3):239–257. doi: 10.1016/s0769-2609(86)80115-6. [DOI] [PubMed] [Google Scholar]
  14. Gottlieb M. S., Schroff R., Schanker H. M., Weisman J. D., Fan P. T., Wolf R. A., Saxon A. Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency. N Engl J Med. 1981 Dec 10;305(24):1425–1431. doi: 10.1056/NEJM198112103052401. [DOI] [PubMed] [Google Scholar]
  15. Horsburgh C. R., Jr, Mason U. G., 3rd, Farhi D. C., Iseman M. D. Disseminated infection with Mycobacterium avium-intracellulare. A report of 13 cases and a review of the literature. Medicine (Baltimore) 1985 Jan;64(1):36–48. doi: 10.1097/00005792-198501000-00003. [DOI] [PubMed] [Google Scholar]
  16. Horsburgh C. R., Jr Mycobacterium avium complex infection in the acquired immunodeficiency syndrome. N Engl J Med. 1991 May 9;324(19):1332–1338. doi: 10.1056/NEJM199105093241906. [DOI] [PubMed] [Google Scholar]
  17. JACKSON S., MORRIS B. C. Enhancement of growth of Pasteurella pestis and other bacteria in serum by the addition of iron. Br J Exp Pathol. 1961 Aug;42:363–368. [PMC free article] [PubMed] [Google Scholar]
  18. Johnson J. L., Shiratsuchi H., Toba H., Ellner J. J. Preservation of monocyte effector functions against Mycobacterium avium-M. intracellulare in patients with AIDS. Infect Immun. 1991 Oct;59(10):3639–3645. doi: 10.1128/iai.59.10.3639-3645.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. KOCHAN I., PATTON C., ISHAK K. TUBERCULOSTATIC ACTIVITY OF NORMAL HUMAN SERA. J Immunol. 1963 May;90:711–719. [PubMed] [Google Scholar]
  20. Querfeld U., Ong J. M., Prehn J., Carty J., Saffari B., Jordan S. C., Kern P. A. Effects of cytokines on the production of lipoprotein lipase in cultured human macrophages. J Lipid Res. 1990 Aug;31(8):1379–1386. [PubMed] [Google Scholar]
  21. Rogers H. J. Bacteriostatic effects of horse sera and serum fractions on Clostridium welchii Type A, and the abolition of bacteriostasis by iron salts. Immunology. 1967 Mar;12(3):285–301. [PMC free article] [PubMed] [Google Scholar]
  22. Schnittman S., Lane H. C., Witebsky F. G., Gosey L. L., Hoggan M. D., Fauci A. S. Host defense against Mycobacterium-avium complex. J Clin Immunol. 1988 Jul;8(4):234–243. doi: 10.1007/BF00916551. [DOI] [PubMed] [Google Scholar]
  23. Shiratsuchi H., Johnson J. L., Ellner J. J. Bidirectional effects of cytokines on the growth of Mycobacterium avium within human monocytes. J Immunol. 1991 May 1;146(9):3165–3170. [PubMed] [Google Scholar]
  24. Stevenson H. C., Schlick E., Griffith R., Chirigos M. A., Brown R., Conlon J., Kanapa D. J., Oldham R. K., Miller P. Characterization of biological response modifier release by human monocytes cultured in suspension in serum-free medium. J Immunol Methods. 1984 May 25;70(2):245–255. doi: 10.1016/0022-1759(84)90189-3. [DOI] [PubMed] [Google Scholar]
  25. Van Lenten B. J., Fogelman A. M. Processing of lipoproteins in human monocyte-macrophages. J Lipid Res. 1990 Aug;31(8):1455–1466. [PubMed] [Google Scholar]
  26. Wheeler P. R., Bulmer K., Ratledge C. Enzymes for biosynthesis de novo and elongation of fatty acids in mycobacteria grown in host cells: is Mycobacterium leprae competent in fatty acid biosynthesis? J Gen Microbiol. 1990 Jan;136(1):211–217. doi: 10.1099/00221287-136-1-211. [DOI] [PubMed] [Google Scholar]
  27. Youdim S. In vitro effect of iron salts and chelating agents on serum tuberculostasis. Am Rev Respir Dis. 1969 Jun;99(6):925–931. doi: 10.1164/arrd.1969.99.6.925. [DOI] [PubMed] [Google Scholar]

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