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. 1992 Feb;60(2):479–484. doi: 10.1128/iai.60.2.479-484.1992

Protective local and systemic antibody responses of swine exposed to an aerosol of Actinobacillus pleuropneumoniae serotype 1.

J T Bossé 1, R P Johnson 1, M Nemec 1, S Rosendal 1
PMCID: PMC257652  PMID: 1730479

Abstract

The isotype-specific antibody responses in serum and in nasal and pulmonary lavage fluids of swine following aerosol immunization with an attenuated strain of Actinobacillus pleuropneumoniae serotype 1, strain CM5A, was investigated. The presence of immunoglobulin G (IgG), IgA, and IgM with specificities for capsular polysaccharide, lipopolysaccharide, and hemolysin was determined by enzyme-linked immunosorbent assay by using purified antigens. Strain CM5A induced serum antibodies of each isotype to the three antigens. The serum antibody response was sustained and typical of persistent antigenic stimulation. The specific IgM response decreased and the specific IgG response increased after challenge with strain CM5. IgA specific for the three antigens was detected in nasal secretions from all immune pigs, whereas specific IgG could only be detected in samples contaminated with blood. Both IgA and IgG specific for each of the antigens were detected in pulmonary lavage samples. There was no significant increase in specific IgA in nasal secretions; however, levels of lipopolysaccharide-specific and hemolysin-specific IgG and IgA in pulmonary secretions rose after aerosol challenge with strain CM5. Passive transfer of immune swine serum resulted in protection against pleuropneumonia and in levels of specific serum IgG which were similar to those in actively immunized pigs. It is concluded that specific serum IgG antibodies are important in protection from porcine pleuropneumonia.

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Selected References

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  1. Bossé J. T., Johnson R. P., Rosendal S. Capsular polysaccharide antigens for detection of serotype-specific antibodies to Actinobacillus pleuropneumoniae. Can J Vet Res. 1990 Jun;54(3):320–325. [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Bradley P. A., Bourne F. J., Brown P. J. The respiratory tract immune system in the pig. II. associated lymphoid tissues. Vet Pathol. 1976;13(2):90–97. doi: 10.1177/030098587601300202. [DOI] [PubMed] [Google Scholar]
  4. Charley B. Local immunity in the pig respiratory tract. I. -- Cellular and humoral immune responses following swine influenza infection. Ann Microbiol (Paris) 1977 Jul;128B(1):95–107. [PubMed] [Google Scholar]
  5. Darveau R. P., Hancock R. E. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol. 1983 Aug;155(2):831–838. doi: 10.1128/jb.155.2.831-838.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Devenish J., Rosendal S., Bossé J. T. Humoral antibody response and protective immunity in swine following immunization with the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae. Infect Immun. 1990 Dec;58(12):3829–3832. doi: 10.1128/iai.58.12.3829-3832.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Devenish J., Rosendal S., Bossé J. T., Wilkie B. N., Johnson R. Prevalence of seroreactors to the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae in swine herds. J Clin Microbiol. 1990 Apr;28(4):789–791. doi: 10.1128/jcm.28.4.789-791.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Devenish J., Rosendal S. Identification of the heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1. Can J Vet Res. 1989 Apr;53(2):251–254. [PMC free article] [PubMed] [Google Scholar]
  9. Devenish J., Rosendal S., Johnson R., Hubler S. Immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in Actinobacillus pleuropneumoniae, Actinobacillus suis, Pasteurella haemolytica, and Escherichia coli. Infect Immun. 1989 Oct;57(10):3210–3213. doi: 10.1128/iai.57.10.3210-3213.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Duncan J. R., Thomson R. G. Influence of immunization procedures on upper respiratory tract immunity in cattle. Can J Comp Med. 1970 Jan;34(1):94–100. [PMC free article] [PubMed] [Google Scholar]
  11. Fenwick B. W., Cullor J. S., Osburn B. I., Olander H. J. Mechanisms involved in protection provided by immunization against core lipopolysaccharides of Escherichia coli J5 from lethal Haemophilus pleuropneumoniae infections in swine. Infect Immun. 1986 Aug;53(2):298–304. doi: 10.1128/iai.53.2.298-304.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fenwick B. W., Osburn B. I. Immune responses to the lipopolysaccharides and capsular polysaccharides of Haemophilus pleuropneumoniae in convalescent and immunized pigs. Infect Immun. 1986 Nov;54(2):575–582. doi: 10.1128/iai.54.2.575-582.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Higgins R., Larivière S., Mittal K. R., Martineau G. P., Rousseau P., Cameron J. Evaluation of a Killed Vaccine Against Porcine Pleuropneumonia Due to Haemophilus pleuropneumoniae. Can Vet J. 1985 Feb;26(2):86–89. [PMC free article] [PubMed] [Google Scholar]
  14. Inzana T. J., Clark G. F., Todd J. Detection of serotype-specific antibodies or capsular antigen of Actinobacillus pleuropneumoniae by a double-label radioimmunoassay. J Clin Microbiol. 1990 Feb;28(2):312–318. doi: 10.1128/jcm.28.2.312-318.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Inzana T. J., Ma J., Workman T., Gogolewski R. P., Anderson P. Virulence properties and protective efficacy of the capsular polymer of Haemophilus (Actinobacillus) pleuropneumoniae serotype 5. Infect Immun. 1988 Aug;56(8):1880–1889. doi: 10.1128/iai.56.8.1880-1889.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Inzana T. J., Mathison B. Serotype specificity and immunogenicity of the capsular polymer of Haemophilus pleuropneumoniae serotype 5. Infect Immun. 1987 Jul;55(7):1580–1587. doi: 10.1128/iai.55.7.1580-1587.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kamp E. M., van Leengoed L. A. Serotype-related differences in production and type of heat-labile hemolysin and heat-labile cytotoxin of Actinobacillus (Haemophilus) pleuropneumoniae. J Clin Microbiol. 1989 Jun;27(6):1187–1191. doi: 10.1128/jcm.27.6.1187-1191.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Macinnes J. I., Rosendal S. Prevention and Control of Actinobacillus (Haemophilus) pleuropneumoniae Infection in Swine: A review. Can Vet J. 1988 Jul;29(7):572–574. [PMC free article] [PubMed] [Google Scholar]
  19. Morgan K. L., Bourne F. J. Immunoglobulin levels in porcine nasal and tracheal secretions--the influence of the method of collection. J Immunol Methods. 1980;37(2):165–173. doi: 10.1016/0022-1759(80)90201-x. [DOI] [PubMed] [Google Scholar]
  20. Morgan K. L., Hussein A. M., Newby T. J., Bourne F. J. Quantification and origin of the immunoglobulins in porcine respiratory tract secretions. Immunology. 1980 Nov;41(3):729–736. [PMC free article] [PubMed] [Google Scholar]
  21. Nielsen R. Haemophilus parahaemolyticus serotypes. Pathogenicity and cross immunity. Nord Vet Med. 1979 Oct;31(10):407–413. [PubMed] [Google Scholar]
  22. Nielsen R. Haemophilus pleuropneumoniae serotypes--cross protection experiments. Nord Vet Med. 1984 Jul-Aug;36(7-8):221–234. [PubMed] [Google Scholar]
  23. Rapp V. J., Ross R. F. Antibody response of swine to outer membrane components of Haemophilus pleuropneumoniae during infection. Infect Immun. 1986 Dec;54(3):751–760. doi: 10.1128/iai.54.3.751-760.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rapp V. J., Ross R. F. Immunogenicity of Outer Membrane Components of Haemophilus (Actinobacillus) pleuropneumoniae. Can Vet J. 1988 Jul;29(7):585–587. [PMC free article] [PubMed] [Google Scholar]
  25. Rosendal S., Carpenter D. S., Mitchell W. R., Wilson M. R. Vaccination against pleuropneumonia of pigs caused by Haemophilus pleuropneumoniae. Can Vet J. 1981 Feb;22(2):34–35. [PMC free article] [PubMed] [Google Scholar]
  26. Rosendal S., Devenish J., MacInnes J. I., Lumsden J. H., Watson S., Xun H. Evaluation of heat-sensitive, neutrophil-toxic, and hemolytic activity of Haemophilus (Actinobacillus) pleuropneumoniae. Am J Vet Res. 1988 Jul;49(7):1053–1058. [PubMed] [Google Scholar]
  27. Rosendal S., MacInnes J. I. Characterization of an attenuated strain of Actinobacillus pleuropneumoniae, serotype 1. Am J Vet Res. 1990 May;51(5):711–717. [PubMed] [Google Scholar]
  28. Rosendal S., Miniats O. P., Sinclair P. Protective efficacy of capsule extracts of Haemophilus pleuropneumoniae in pigs and mice. Vet Microbiol. 1986 Sep;12(3):229–240. doi: 10.1016/0378-1135(86)90052-0. [DOI] [PubMed] [Google Scholar]
  29. Sebunya T. N., Saunders J. R., Osborne A. D. Dose response relationship of Haemophilus pleuropneumoniae aerosols in pigs. Can J Comp Med. 1983 Jan;47(1):54–56. [PMC free article] [PubMed] [Google Scholar]

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