Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Feb;60(2):687–689. doi: 10.1128/iai.60.2.687-689.1992

Clustering of an outer membrane adhesin of Haemophilus parainfluenzae.

W F Liljemark 1, C G Bloomquist 1, C H Lai 1
PMCID: PMC257685  PMID: 1730505

Abstract

Haemophilus parainfluenzae synthesizes an outer membrane protein adhesin which mediates binding to oral streptococci, salivary pellicle, and neuraminidase-treated erythrocytes. An indirect gold labeling technique and immunoelectron microscopy verified the location of this outer membrane protein. Further, a clustering of gold particles was observed in irregular patches at the cell surface.

Full text

PDF
687

Images in this article

688Image
on p.688

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolstad A. I., Kristoffersen T., Olsen I., Preus H. R., Jesen H. B., Vasstrand E. N., Bakken V. Outer membrane proteins of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus studied by SDS-PAGE and immunoblotting. Oral Microbiol Immunol. 1990 Jun;5(3):155–161. doi: 10.1111/j.1399-302x.1990.tb00414.x. [DOI] [PubMed] [Google Scholar]
  2. Boyd J., McBride B. C. Fractionation of hemagglutinating and bacterial binding adhesins of Bacteroides gingivalis. Infect Immun. 1984 Aug;45(2):403–409. doi: 10.1128/iai.45.2.403-409.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cisar J. O., Kolenbrander P. E., McIntire F. C. Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. Infect Immun. 1979 Jun;24(3):742–752. doi: 10.1128/iai.24.3.742-752.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Devine D. A., Gmür R., Handley P. S. Ultrastructure, serogrouping and localization of surface antigens of Bacteroides intermedius. J Gen Microbiol. 1989 Apr;135(4):967–979. doi: 10.1099/00221287-135-4-967. [DOI] [PubMed] [Google Scholar]
  5. Doig P., Todd T., Sastry P. A., Lee K. K., Hodges R. S., Paranchych W., Irvin R. T. Role of pili in adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells. Infect Immun. 1988 Jun;56(6):1641–1646. doi: 10.1128/iai.56.6.1641-1646.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Evans D. G., Evans D. J., Jr, Moulds J. J., Graham D. Y. N-acetylneuraminyllactose-binding fibrillar hemagglutinin of Campylobacter pylori: a putative colonization factor antigen. Infect Immun. 1988 Nov;56(11):2896–2906. doi: 10.1128/iai.56.11.2896-2906.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fives-Taylor P. M., Macrina F. L., Pritchard T. J., Peene S. S. Expression of Streptococcus sanguis antigens in Escherichia coli: cloning of a structural gene for adhesion fimbriae. Infect Immun. 1987 Jan;55(1):123–128. doi: 10.1128/iai.55.1.123-128.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ganeshkumar N., Song M., McBride B. C. Cloning of a Streptococcus sanguis adhesin which mediates binding to saliva-coated hydroxyapatite. Infect Immun. 1988 May;56(5):1150–1157. doi: 10.1128/iai.56.5.1150-1157.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gibbons R. J. Adherent interactions which may affect microbial ecology in the mouth. J Dent Res. 1984 Mar;63(3):378–385. doi: 10.1177/00220345840630030401. [DOI] [PubMed] [Google Scholar]
  10. Gilleland H. E., Jr, Matthews-Greer J. M. Perspectives on the potential for successful development of outer membrane protein vaccines. Eur J Clin Microbiol. 1987 Jun;6(3):231–233. doi: 10.1007/BF02017606. [DOI] [PubMed] [Google Scholar]
  11. Grenier D., Mayrand D. Functional characterization of extracellular vesicles produced by Bacteroides gingivalis. Infect Immun. 1987 Jan;55(1):111–117. doi: 10.1128/iai.55.1.111-117.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holmans P. L., Loftus T. A., Hansen E. J. Cloning and surface expression in Escherichia coli of a structural gene encoding a surface protein of Haemophilus influenzae type b. Infect Immun. 1985 Oct;50(1):236–242. doi: 10.1128/iai.50.1.236-242.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holt S. C., Tanner A. C., Socransky S. S. Morphology and ultrastructure of oral strains of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus. Infect Immun. 1980 Nov;30(2):588–600. doi: 10.1128/iai.30.2.588-600.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Isogai H., Isogai E., Yoshimura F., Suzuki T., Kagota W., Takano K. Specific inhibition of adherence of an oral strain of Bacteroides gingivalis 381 to epithelial cells by monoclonal antibodies against the bacterial fimbriae. Arch Oral Biol. 1988;33(7):479–485. doi: 10.1016/0003-9969(88)90028-3. [DOI] [PubMed] [Google Scholar]
  15. Kaufman J., DiRienzo J. M. Isolation of a corncob (coaggregation) receptor polypeptide from Fusobacterium nucleatum. Infect Immun. 1989 Feb;57(2):331–337. doi: 10.1128/iai.57.2.331-337.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lai C. H., Bloomquist C., Liljemark W. F. Purification and characterization of an outer membrane protein adhesin from Haemophilus parainfluenzae HP-28. Infect Immun. 1990 Dec;58(12):3833–3839. doi: 10.1128/iai.58.12.3833-3839.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lindberg F., Lund B., Johansson L., Normark S. Localization of the receptor-binding protein adhesin at the tip of the bacterial pilus. Nature. 1987 Jul 2;328(6125):84–87. doi: 10.1038/328084a0. [DOI] [PubMed] [Google Scholar]
  18. London J., Allen J. Purification and characterization of a Bacteroides loeschei adhesin that interacts with procaryotic and eucaryotic cells. J Bacteriol. 1990 May;172(5):2527–2534. doi: 10.1128/jb.172.5.2527-2534.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. London J., Hand A. R., Weiss E. I., Allen J. Bacteroides loeschei PK1295 cells express two distinct adhesins simultaneously. Infect Immun. 1989 Dec;57(12):3940–3944. doi: 10.1128/iai.57.12.3940-3944.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McIntire F. C., Crosby L. K., Vatter A. E., Cisar J. O., McNeil M. R., Bush C. A., Tjoa S. S., Fennessey P. V. A polysaccharide from Streptococcus sanguis 34 that inhibits coaggregation of S. sanguis 34 with Actinomyces viscosus T14V. J Bacteriol. 1988 May;170(5):2229–2235. doi: 10.1128/jb.170.5.2229-2235.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Moch T., Hoschützky H., Hacker J., Kröncke K. D., Jann K. Isolation and characterization of the alpha-sialyl-beta-2,3-galactosyl-specific adhesin from fimbriated Escherichia coli. Proc Natl Acad Sci U S A. 1987 May;84(10):3462–3466. doi: 10.1073/pnas.84.10.3462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Morton D. J., Williams P. Characterization of the outer-membrane proteins of Haemophilus parainfluenzae expressed under iron-sufficient and iron-restricted conditions. J Gen Microbiol. 1989 Feb;135(Pt 2):445–451. doi: 10.1099/00221287-135-2-445. [DOI] [PubMed] [Google Scholar]
  23. Mouton C., Bouchard D., Deslauriers M., Lamonde L. Immunochemical identification and preliminary characterization of a nonfimbrial hemagglutinating adhesin of Bacteroides gingivalis. Infect Immun. 1989 Feb;57(2):566–573. doi: 10.1128/iai.57.2.566-573.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Murray P. A., Kern D. G., Winkler J. R. Identification of a galactose-binding lectin on Fusobacterium nucleatum FN-2. Infect Immun. 1988 May;56(5):1314–1319. doi: 10.1128/iai.56.5.1314-1319.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Nyberg G., Strömberg N., Jonsson A., Karlsson K. A., Normark S. Erythrocyte gangliosides act as receptors for Neisseria subflava: identification of the Sia-1 adhesin. Infect Immun. 1990 Aug;58(8):2555–2563. doi: 10.1128/iai.58.8.2555-2563.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Paruchuri D. K., Seifert H. S., Ajioka R. S., Karlsson K. A., So M. Identification and characterization of a Neisseria gonorrhoeae gene encoding a glycolipid-binding adhesin. Proc Natl Acad Sci U S A. 1990 Jan;87(1):333–337. doi: 10.1073/pnas.87.1.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Revis G. J., Vatter A. E., Crowle A. J., Cisar J. O. Antibodies against the Ag2 fimbriae of Actinomyces viscosus T14V inhibit lactose-sensitive bacterial adherence. Infect Immun. 1982 Jun;36(3):1217–1222. doi: 10.1128/iai.36.3.1217-1222.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weiss E. I., London J., Kolenbrander P. E., Hand A. R., Siraganian R. Localization and enumeration of fimbria-associated adhesins of Bacteroides loescheii. J Bacteriol. 1988 Mar;170(3):1123–1128. doi: 10.1128/jb.170.3.1123-1128.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weiss E. I., London J., Kolenbrander P. E., Kagermeier A. S., Andersen R. N. Characterization of lectinlike surface components on Capnocytophaga ochracea ATCC 33596 that mediate coaggregation with gram-positive oral bacteria. Infect Immun. 1987 May;55(5):1198–1202. doi: 10.1128/iai.55.5.1198-1202.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. van der Ley P., Kuipers O., Tommassen J., Lugtenberg B. O-antigenic chains of lipopolysaccharide prevent binding of antibody molecules to an outer membrane pore protein in Enterobacteriaceae. Microb Pathog. 1986 Feb;1(1):43–49. doi: 10.1016/0882-4010(86)90030-6. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES