Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1992 Feb;60(2):715–719. doi: 10.1128/iai.60.2.715-719.1992

Polyclonal cell activity of a repeat peptide derived from the sequence of an 85-kilodalton surface protein of Trypanosoma cruzi trypomastigotes.

J Pestel 1, J P Defoort 1, H Gras-Masse 1, D Afchain 1, A Capron 1, A Tartar 1, A Ouaissi 1
PMCID: PMC257692  PMID: 1730508

Abstract

Some in vitro and in vivo biological activities of an octadecapeptide derived from an 85-kDa surface protein of Trypanosoma cruzi trypomastigote were studied. The peptide coupled to a carrier protein induced the proliferative response of lymph node cells from mice immunized with various antigens. Moreover, sera from mice immunized with the coupled peptide were found to contain antibodies against a number of self and nonself antigens: fibronectin, bovine serum albumin, myosin, tetanus toxoid, ovalbumin, keyhole limpet hemocyanin, and DNA. These results are discussed in the context of Chagas' disease immunopathology.

Full text

PDF
715

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auriault C., Gras-Masse H., Wolowczuk I., Pierce R. J., Balloul J. M., Neyrinck J. L., Drobecq H., Tartar A., Capron A. Analysis of T and B cell epitopes of the Schistosoma mansoni P28 antigen in the rat model by using synthetic peptides. J Immunol. 1988 Sep 1;141(5):1687–1694. [PubMed] [Google Scholar]
  2. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  3. Corradin G., Etlinger H. M., Chiller J. M. Lymphocyte specificity to protein antigens. I. Characterization of the antigen-induced in vitro T cell-dependent proliferative response with lymph node cells from primed mice. J Immunol. 1977 Sep;119(3):1048–1053. [PubMed] [Google Scholar]
  4. Cunningham D. S., Grogl M., Kuhn R. E. Suppression of antibody responses in humans infected with Trypanosoma cruzi. Infect Immun. 1980 Nov;30(2):496–499. doi: 10.1128/iai.30.2.496-499.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GOODFRIEND T. L., LEVINE L., FASMAN G. D. ANTIBODIES TO BRADYKININ AND ANGIOTENSIN: A USE OF CARBODIIMIDES IN IMMUNOLOGY. Science. 1964 Jun 12;144(3624):1344–1346. doi: 10.1126/science.144.3624.1344. [DOI] [PubMed] [Google Scholar]
  6. Hoffman S. L., Cannon L. T., Sr, Berzofsky J. A., Majarian W. R., Young J. F., Maloy W. L., Hockmeyer W. T. Plasmodium falciparum: sporozoite boosting of immunity due to a T-cell epitope on a sporozoite vaccine. Exp Parasitol. 1987 Aug;64(1):64–70. doi: 10.1016/0014-4894(87)90009-9. [DOI] [PubMed] [Google Scholar]
  7. Khoury E. L., Ritacco V., Cossio P. M., Laguens R. P., Szarfman A., Diez C., Arana R. M. Circulating antibodies to peripheral nerve in American trypanosomiasis (Chagas' disease). Clin Exp Immunol. 1979 Apr;36(1):8–15. [PMC free article] [PubMed] [Google Scholar]
  8. Kierszenbaum F. Is there autoimmunity in Chagas disease? Parasitol Today. 1985 Jul;1(1):4–6. doi: 10.1016/0169-4758(85)90098-5. [DOI] [PubMed] [Google Scholar]
  9. Lee A. C., Powell J. E., Tregear G. W., Niall H. D., Stevens V. C. A method for preparing beta-hCG COOH peptide-carrier conjugates of predictable composition. Mol Immunol. 1980 Jun;17(6):749–756. doi: 10.1016/0161-5890(80)90145-5. [DOI] [PubMed] [Google Scholar]
  10. Liew F. Y., Millott S. M., Schmidt J. A. A repetitive peptide of Leishmania can activate T helper type 2 cells and enhance disease progression. J Exp Med. 1990 Nov 1;172(5):1359–1365. doi: 10.1084/jem.172.5.1359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Liew F. Y., Schmidt J. A., Liu D. S., Millott S. M., Scott M. T., Dhaliwal J. S., Croft S. L. Suppressive substance produced by T cells from mice chronically infected with Trypanosoma cruzi. II. Partial biochemical characterization. J Immunol. 1988 Feb 1;140(3):969–973. [PubMed] [Google Scholar]
  12. McCormick T. S., Rowland E. C. Trypanosoma cruzi: cross-reactive anti-heart autoantibodies produced during infection in mice. Exp Parasitol. 1989 Nov;69(4):393–401. doi: 10.1016/0014-4894(89)90088-x. [DOI] [PubMed] [Google Scholar]
  13. Minoprio P. M., Eisen H., Forni L., D'Imperio Lima M. R., Joskowicz M., Coutinho A. Polyclonal lymphocyte responses to murine Trypanosoma cruzi infection. I. Quantitation of both T- and B-cell responses. Scand J Immunol. 1986 Dec;24(6):661–668. doi: 10.1111/j.1365-3083.1986.tb02185.x. [DOI] [PubMed] [Google Scholar]
  14. Ortiz-Ortiz L., Parks D. E., Rodriguez M., Weigle W. O. Polyclonal B lymphocyte activation during Trypanosoma cruzi infection. J Immunol. 1980 Jan;124(1):121–126. [PubMed] [Google Scholar]
  15. Ouaissi A., Cornette J., Velge P., Capron A. Identification of anti-acetylcholinesterase and anti-idiotype antibodies in human and experimental Chagas' disease: pathological implications. Eur J Immunol. 1988 Dec;18(12):1889–1894. doi: 10.1002/eji.1830181204. [DOI] [PubMed] [Google Scholar]
  16. Ouaissi M. A., Cornette J., Afchain D., Capron A., Gras-Masse H., Tartar A. Trypanosoma cruzi infection inhibited by peptides modeled from a fibronectin cell attachment domain. Science. 1986 Oct 31;234(4776):603–607. doi: 10.1126/science.3094145. [DOI] [PubMed] [Google Scholar]
  17. Ouaissi M. A., Taibi A., Cornette J., Velge P., Marty B., Loyens M., Esteva M., Rizvi F. S., Capron A. Characterization of major surface and excretory-secretory immunogens of Trypanosoma cruzi trypomastigotes and identification of potential protective antigen. Parasitology. 1990 Feb;100(Pt 1):115–124. doi: 10.1017/s0031182000060182. [DOI] [PubMed] [Google Scholar]
  18. Pestel J., Dissous C., Dessaint J. P., Louis J., Engers H., Capron A. Specific Schistosoma mansoni rat T cell clones. I. Generation and functional analysis in vitro and in vivo. J Immunol. 1985 Jun;134(6):4132–4139. [PubMed] [Google Scholar]
  19. Pestel J., Dissous C., Louis J., Kusnierz J. P., Damonneville M., Dessaint J. P., Capron A. Schistosoma mansoni-specific rat T cell clones. II. Different effects of adult worm-specific T cell clones in immunocompetent and nude infected rats. Eur J Immunol. 1989 Aug;19(8):1457–1462. doi: 10.1002/eji.1830190817. [DOI] [PubMed] [Google Scholar]
  20. Peterson D. S., Wrightsman R. A., Manning J. E. Cloning of a major surface-antigen gene of Trypanosoma cruzi and identification of a nonapeptide repeat. Nature. 1986 Aug 7;322(6079):566–568. doi: 10.1038/322566a0. [DOI] [PubMed] [Google Scholar]
  21. Petry K., Eisen H. Chagas disease: a model for the study of autoimmune diseases. Parasitol Today. 1989 Apr;5(4):111–116. doi: 10.1016/0169-4758(89)90052-5. [DOI] [PubMed] [Google Scholar]
  22. Reed S. G., Inverso J. A., Roters S. B. Heterologous antibody responses in mice with chronic T. cruzi infection: depressed T helper function restored with supernatants containing interleukin 2. J Immunol. 1984 Sep;133(3):1558–1563. [PubMed] [Google Scholar]
  23. Serrano L. E., O'Daly J. A. Protein fraction from Trypanosoma cruzi infected spleen cell supernatants with immunosuppressive activity in vitro. Int J Parasitol. 1987 Feb;17(3):851–861. doi: 10.1016/0020-7519(87)90069-5. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES