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. 1991 Jan;59(1):398–406. doi: 10.1128/iai.59.1.398-406.1991

Prostaglandin E release from human monocytes treated with lipopolysaccharides isolated from Bacteroides intermedius and Salmonella typhimurium: potentiation by gamma interferon.

F C Nichols 1, J F Peluso 1, P J Tempro 1, S W Garrison 1, J B Payne 1
PMCID: PMC257754  PMID: 1898900

Abstract

The purpose of this investigation was to examine gamma interferon potentiation of lipopolysaccharide (LPS) responses in human monocytes by using phenol-water-extracted (unfractionated) and highly purified LPS preparations isolated from Bacteroides intermedius and Salmonella typhimurium. Phenol-water-extracted LPS preparations from these bacteria were further purified by chromatography over Sepharose-CL-4B. LPS enrichment in pooled column fractions was assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and quantitation of hydroxy-fatty acid and 2-keto-3-deoxyoctulosonic acid content, protein contamination, and anthrone-reactive material. Monocyte stimulation by LPS, measured as prostaglandin E (PGE) release, was assessed with and without gamma interferon treatment. Cells were either treated simultaneously with gamma interferon and LPS or pretreated with gamma interferon prior to LPS stimulation. PGE release from counterflow-isolated monocytes was quantitated during the 0- to 24-h and 24- to 48-h culture intervals. Contrary to previous results from this laboratory, phenol-water-extracted LPS preparations from B. intermedius and S. typhimurium were similar in their capacities to stimulate PGE release from monocytes. Molecular sieve chromatography was found to remove substantial amounts of high-molecular-weight polysaccharide contaminants only from the B. intermedius LPS but did not significantly alter the potency of either B. intermedius or S. typhimurium LPS. Gamma interferon cotreatment did not potentiate the release of PGE with any of the LPS preparations tested. However, 24-h pretreatment of monocytes with gamma interferon followed by a 24-h exposure to LPS resulted in significant potentiation of PGE release over LPS alone. In addition, B. intermedium preparations were approximately threefold more potent than similarly prepared LPS isolates from S. typhimurium following gamma interferon pretreatment. These results indicate that gamma interferon can selectively potentiate the effects of B. intermedius LPS in human monocyte isolates.

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Selected References

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  1. Arenzana-Seisdedos F., Virelizier J. L., Fiers W. Interferons as macrophage-activating factors. III. Preferential effects of interferon-gamma on the interleukin 1 secretory potential of fresh or aged human monocytes. J Immunol. 1985 Apr;134(4):2444–2448. [PubMed] [Google Scholar]
  2. Blackshear P. J. Systems for polyacrylamide gel electrophoresis. Methods Enzymol. 1984;104:237–255. doi: 10.1016/s0076-6879(84)04093-3. [DOI] [PubMed] [Google Scholar]
  3. Boraschi D., Censini S., Bartalini M., Tagliabue A. Regulation of arachidonic acid metabolism in macrophages by immune and nonimmune interferons. J Immunol. 1985 Jul;135(1):502–505. [PubMed] [Google Scholar]
  4. Brunk C. F., Jones K. C., James T. W. Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem. 1979 Jan 15;92(2):497–500. doi: 10.1016/0003-2697(79)90690-0. [DOI] [PubMed] [Google Scholar]
  5. Garrison S. W., Holt S. C., Nichols F. C. Lipopolysaccharide-stimulated PGE2 release from human monocytes. Comparison of lipopolysaccharides prepared from suspected periodontal pathogens. J Periodontol. 1988 Oct;59(10):684–687. doi: 10.1902/jop.1988.59.10.684. [DOI] [PubMed] [Google Scholar]
  6. Garrison S. W., Nichols F. C. Gamma interferon modulation of prostaglandin E2 release from monocytes stimulated with lipopolysaccharides from Bacteroides intermedius, Bacteroides gingivalis, and Salmonella typhimurium. Oral Microbiol Immunol. 1988 Sep;3(3):138–143. doi: 10.1111/j.1399-302x.1988.tb00099.x. [DOI] [PubMed] [Google Scholar]
  7. Hamilton T. A., Rigsbee J. E., Scott W. A., Adams D. O. gamma-Interferon enhances the secretion of arachidonic acid metabolites from murine peritoneal macrophages stimulated with phorbol diesters. J Immunol. 1985 Apr;134(4):2631–2636. [PubMed] [Google Scholar]
  8. Iino Y., Hopps R. M. The bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Capnocytophaga ochracea isolated from human mouths. Arch Oral Biol. 1984;29(1):59–63. doi: 10.1016/0003-9969(84)90043-8. [DOI] [PubMed] [Google Scholar]
  9. Johne B., Bryn K. Chemical composition and biological properties of a lipopolysaccharide from Bacteroides intermedius. Acta Pathol Microbiol Immunol Scand B. 1986 Aug;94(4):265–271. doi: 10.1111/j.1699-0463.1986.tb03051.x. [DOI] [PubMed] [Google Scholar]
  10. Johne B., Olsen I., Bryn K. Fatty acids and sugars in lipoplysaccharides from Bacteroides intermedius. Bacteroides gingivalis and Bacteroides loescheii. Oral Microbiol Immunol. 1988 Mar;3(1):22–27. doi: 10.1111/j.1399-302x.1988.tb00600.x. [DOI] [PubMed] [Google Scholar]
  11. Karkhanis Y. D., Zeltner J. Y., Jackson J. J., Carlo D. J. A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem. 1978 Apr;85(2):595–601. doi: 10.1016/0003-2697(78)90260-9. [DOI] [PubMed] [Google Scholar]
  12. Koga T., Nishihara T., Fujiwara T., Nisizawa T., Okahashi N., Noguchi T., Hamada S. Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli. Infect Immun. 1985 Mar;47(3):638–647. doi: 10.1128/iai.47.3.638-647.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mansheim B. J., Onderdonk A. B., Kasper D. L. Immunochemical and biologic studies of the lipopolysaccharide of Bacteroides melaninogenicus subspecies asaccharolyticus. J Immunol. 1978 Jan;120(1):72–78. [PubMed] [Google Scholar]
  14. Mashimo J., Yoshida M., Ikeuchi K., Hata S., Arata S., Kasai N., Okuda K., Takazoe I. Fatty acid composition and Shwartzman activity of lipopolysaccharides from oral bacteria. Microbiol Immunol. 1985;29(5):395–403. doi: 10.1111/j.1348-0421.1985.tb00840.x. [DOI] [PubMed] [Google Scholar]
  15. Mayberry W. R. Cellular distribution and linkage of D-(-)-3-hydroxy fatty acids in Bacteroides species. J Bacteriol. 1980 Oct;144(1):200–204. doi: 10.1128/jb.144.1.200-204.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mayberry W. R. Hydroxy fatty acids in Bacteroides species: D-(--)-3-hydroxy-15-methylhexadecanoate and its homologs. J Bacteriol. 1980 Aug;143(2):582–587. doi: 10.1128/jb.143.2.582-587.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McKeehan W. L., McKeehan K. A., Hammond S. L., Ham R. G. Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein. In Vitro. 1977 Jul;13(7):399–416. doi: 10.1007/BF02615100. [DOI] [PubMed] [Google Scholar]
  18. Merril C. R., Goldman D., Van Keuren M. L. Silver staining methods for polyacrylamide gel electrophoresis. Methods Enzymol. 1983;96:230–239. doi: 10.1016/s0076-6879(83)96021-4. [DOI] [PubMed] [Google Scholar]
  19. Millar S. J., Goldstein E. G., Levine M. J., Hausmann E. Modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from Bacteroides gingivalis. Infect Immun. 1986 Jan;51(1):302–306. doi: 10.1128/iai.51.1.302-306.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nair B. C., Mayberry W. R., Dziak R., Chen P. B., Levine M. J., Hausmann E. Biological effects of a purified lipopolysaccharide from Bacteroides gingivalis. J Periodontal Res. 1983 Jan;18(1):40–49. doi: 10.1111/j.1600-0765.1983.tb00333.x. [DOI] [PubMed] [Google Scholar]
  21. Nichols F. C., Garrison S. W., Davis H. W. Prostaglandin E2 and thromboxane B2 release from human monocytes treated with bacterial lipopolysaccharide. J Leukoc Biol. 1988 Nov;44(5):376–384. doi: 10.1002/jlb.44.5.376. [DOI] [PubMed] [Google Scholar]
  22. Nichols F. C., Garrison S. W. Interferon-gamma potentiation of lipopolysaccharide-induced eicosanoid release from human monocytes. J Interferon Res. 1987 Feb;7(1):121–129. doi: 10.1089/jir.1987.7.121. [DOI] [PubMed] [Google Scholar]
  23. Okuda K., Kato T. Hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria. Infect Immun. 1987 Dec;55(12):3192–3196. doi: 10.1128/iai.55.12.3192-3196.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. ROE J. H. The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem. 1955 Jan;212(1):335–343. [PubMed] [Google Scholar]
  25. Schifferle R. E., Reddy M. S., Zambon J. J., Genco R. J., Levine M. J. Characterization of a polysaccharide antigen from Bacteroides gingivalis. J Immunol. 1989 Nov 1;143(9):3035–3042. [PubMed] [Google Scholar]
  26. Sveen K. The capacity of lipopolysaccharides from bacteroides, fusobacterium and veillonella to produce skin inflammation and the local and generalized Shwartzman reaction in rabbits. J Periodontal Res. 1977 Sep;12(5):340–350. doi: 10.1111/j.1600-0765.1977.tb01525.x. [DOI] [PubMed] [Google Scholar]
  27. Vukajlovich S. W., Morrison D. C. Conversion of lipopolysaccharides to molecular aggregates with reduced subunit heterogeneity: demonstration of LPS-responsiveness in "endotoxin-unresponsive" C3H/HeJ splenocytes. J Immunol. 1983 Jun;130(6):2804–2808. [PubMed] [Google Scholar]

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