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. 1991 Feb;59(2):587–591. doi: 10.1128/iai.59.2.587-591.1991

Efficacy of recombinant leukotoxin in protection against pneumonic challenge with live Pasteurella haemolytica A1.

J A Conlon 1, P E Shewen 1, R Y Lo 1
PMCID: PMC257793  PMID: 1987075

Abstract

The recombinant leukotoxin (rLKT) of the bacterium Pasteurella haemolytica A1 was examined for its ability to protect cattle from experimental challenge with logarithmic-phase P. haemolytica. Six different vaccines were utilized in the experiment: P. haemolytica culture supernatant, P. haemolytica culture supernatant enriched with rLKT, rLKT alone, P. haemolytica culture supernatant enriched with Escherichia coli supernatant not containing LKT, E. coli supernatant alone, and phosphate-buffered saline. rLKT alone showed no protective capacity against development of clinical signs of respiratory disease or against development of postmortem lung lesions after experimental challenge. It was, however, shown to enhance the efficacy of the culture supernatant vaccine and decrease clinical signs and pneumonic lesions. The complexity of protective immunity in this disease is emphasized in this study, and, although LKT is an important virulence factor of the organism, an immune response to LKT alone does not protect animals against disease.

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Selected References

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  1. Adlam C., Knights J. M., Mugridge A., Lindon J. C., Baker P. R., Beesley J. E., Spacey B., Craig G. R., Nagy L. K. Purification, characterization and immunological properties of the serotype-specific capsular polysaccharide of Pasteurella haemolytica (serotype A1) organisms. J Gen Microbiol. 1984 Sep;130(9):2415–2426. doi: 10.1099/00221287-130-9-2415. [DOI] [PubMed] [Google Scholar]
  2. Cardella M. A., Adviento M. A., Nervig R. M. Vaccination studies against experimental bovine Pasteurella pneumonia. Can J Vet Res. 1987 Apr;51(2):204–211. [PMC free article] [PubMed] [Google Scholar]
  3. Chang Y. F., Young R., Post D., Struck D. K. Identification and characterization of the Pasteurella haemolytica leukotoxin. Infect Immun. 1987 Oct;55(10):2348–2354. doi: 10.1128/iai.55.10.2348-2354.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Felmlee T., Welch R. A. Alterations of amino acid repeats in the Escherichia coli hemolysin affect cytolytic activity and secretion. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5269–5273. doi: 10.1073/pnas.85.14.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Frank G. H., Smith P. C. Prevalence of Pasteurella haemolytica in transported calves. Am J Vet Res. 1983 Jun;44(6):981–985. [PubMed] [Google Scholar]
  6. Frank G. H., Tabatabai L. B. Neuraminidase activity of Pasteurella haemolytica isolates. Infect Immun. 1981 Jun;32(3):1119–1122. doi: 10.1128/iai.32.3.1119-1122.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gentry M. J., Confer A. W., Panciera R. J. Serum neutralization of cytotoxin from Pasteurella haemolytica, serotype 1 and resistance to experimental bovine pneumonic pasteurellosis. Vet Immunol Immunopathol. 1985 Jul;9(3):239–250. doi: 10.1016/0165-2427(85)90074-1. [DOI] [PubMed] [Google Scholar]
  8. Gentry M. J., Corstvet R. E., Panciera R. J. Extraction of capsular material from Pasteurella haemolytica. Am J Vet Res. 1982 Nov;43(11):2070–2073. [PubMed] [Google Scholar]
  9. Gonzalez-Rayos C., Lo R. Y., Shewen P. E., Beveridge T. J. Cloning of a serotype-specific antigen from Pasteurella haemolytica A1. Infect Immun. 1986 Sep;53(3):505–510. doi: 10.1128/iai.53.3.505-510.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Himmel M. E., Yates M. D., Lauerman L. H., Squire P. G. Purification and partial characterization of a macrophage cytotoxin from Pasteurella haemolytica. Am J Vet Res. 1982 May;43(5):764–767. [PubMed] [Google Scholar]
  11. Jericho K. W., Langford E. V. Aerosol vaccination of calves with pasteurella haemolytica against experimental respiratory disease. Can J Comp Med. 1982 Jul;46(3):287–292. [PMC free article] [PubMed] [Google Scholar]
  12. Kaehler K. L., Markam R. J., Muscoplat C. C., Johnson D. W. Evidence of cytocidal effects of Pasteurella haemolytica on bovine peripheral blood mononuclear leukocytes. Am J Vet Res. 1980 Oct;41(10):1690–1693. [PubMed] [Google Scholar]
  13. Lo R. Y., Cameron L. A. A simple immunological detection method for the direct screening of genes from clone banks. Biochem Cell Biol. 1986 Jan;64(1):73–76. doi: 10.1139/o86-012. [DOI] [PubMed] [Google Scholar]
  14. Lo R. Y. Molecular characterization of cytotoxins produced by Haemophilus, Actinobacillus, Pasteurella. Can J Vet Res. 1990 Apr;54 (Suppl):S33–S35. [PubMed] [Google Scholar]
  15. Lo R. Y., Shewen P. E., Strathdee C. A., Greer C. N. Cloning and expression of the leukotoxin gene of Pasteurella haemolytica A1 in Escherichia coli K-12. Infect Immun. 1985 Dec;50(3):667–671. doi: 10.1128/iai.50.3.667-671.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lo R. Y., Strathdee C. A., Shewen P. E. Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1. Infect Immun. 1987 Sep;55(9):1987–1996. doi: 10.1128/iai.55.9.1987-1996.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lopez A., Maxie M. G., Savan M., Ruhnke H. L., Thomson R. G., Barnum D. A., Geissinger H. D. The pulmonary clearance of Pasteurella haemolytica in calves infected with bovine virus diarrhea or Mycoplasma bovis. Can J Comp Med. 1982 Jul;46(3):302–306. [PMC free article] [PubMed] [Google Scholar]
  18. Martin S. W., Bateman K. G., Shewen P. E., Rosendal S., Bohac J. E. The frequency, distribution and effects of antibodies, to seven putative respiratory pathogens, on respiratory disease and weight gain in feedlot calves in Ontario. Can J Vet Res. 1989 Jul;53(3):355–362. [PMC free article] [PubMed] [Google Scholar]
  19. Martin S. W., Meek A. H., Davis D. G., Thomson R. G., Johnson J. A., Lopez A., Stephens L., Curtis R. A., Prescott J. F., Rosendal S. Factors associated with mortality in feedlot cattle: the Bruce County Beef Cattle Project. Can J Comp Med. 1980 Jan;44(1):1–10. [PMC free article] [PubMed] [Google Scholar]
  20. Morck D. W., Raybould T. J., Acres S. D., Babiuk L. A., Nelligan J., Costerton J. W. Electron microscopic description of glycocalyx and fimbriae on the surface of Pasteurella haemolytica-A1. Can J Vet Res. 1987 Jan;51(1):83–88. [PMC free article] [PubMed] [Google Scholar]
  21. Morck D. W., Watts T. C., Acres S. D., Costerton J. W. Electron microscopic examination of cells of Pasteurella haemolytica-A1 in experimentally infected cattle. Can J Vet Res. 1988 Jul;52(3):343–348. [PMC free article] [PubMed] [Google Scholar]
  22. Mosier D. A., Confer A. W., Hall S. M., Gentry M. J., Panciera R. J. Enzyme-linked immunosorbent assay for detection of serum antibodies to Pasteurella haemolytica cytotoxin (leukotoxin) in cattle. J Clin Microbiol. 1986 Aug;24(2):218–222. doi: 10.1128/jcm.24.2.218-222.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mosier D. A., Simons K. R., Confer A. W., Panciera R. J., Clinkenbeard K. D. Pasteurella haemolytica antigens associated with resistance to pneumonic pasteurellosis. Infect Immun. 1989 Mar;57(3):711–716. doi: 10.1128/iai.57.3.711-716.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Otulakowski G. L., Shewen P. E., Udoh A. E., Mellors A., Wilkie B. N. Proteolysis of sialoglycoprotein by Pasteurella haemolytica cytotoxic culture supernatant. Infect Immun. 1983 Oct;42(1):64–70. doi: 10.1128/iai.42.1.64-70.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Panciera R. J., Corstvet R. E. Bovine pneumonic pasteurellosis: model for Pasteurella haemolytica- and Pasteurella multocida-induced pneumonia in cattle. Am J Vet Res. 1984 Dec;45(12):2532–2537. [PubMed] [Google Scholar]
  26. Scheirer C. J., Ray W. S., Hare N. The analysis of ranked data derived from completely randomized factorial designs. Biometrics. 1976 Jun;32(2):429–434. [PubMed] [Google Scholar]
  27. Shewen P. E., Wilkie B. N. Antibody titers to Pasteurella haemolytica A1 in Ontario beef cattle. Can J Comp Med. 1982 Oct;46(4):354–356. [PMC free article] [PubMed] [Google Scholar]
  28. Shewen P. E., Wilkie B. N. Cytotoxin of Pasteurella haemolytica acting on bovine leukocytes. Infect Immun. 1982 Jan;35(1):91–94. doi: 10.1128/iai.35.1.91-94.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shewen P. E., Wilkie B. N. Vaccination of calves with leukotoxic culture supernatant from Pasteurella haemolytica. Can J Vet Res. 1988 Jan;52(1):30–36. [PMC free article] [PubMed] [Google Scholar]
  30. Slocombe R. F., Malark J., Ingersoll R., Derksen F. J., Robinson N. E. Importance of neutrophils in the pathogenesis of acute pneumonic pasteurellosis in calves. Am J Vet Res. 1985 Nov;46(11):2253–2258. [PubMed] [Google Scholar]
  31. Stark M. J. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli. Gene. 1987;51(2-3):255–267. doi: 10.1016/0378-1119(87)90314-3. [DOI] [PubMed] [Google Scholar]
  32. Strathdee C. A., Lo R. Y. Cloning, nucleotide sequence, and characterization of genes encoding the secretion function of the Pasteurella haemolytica leukotoxin determinant. J Bacteriol. 1989 Feb;171(2):916–928. doi: 10.1128/jb.171.2.916-928.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Strathdee C. A., Lo R. Y. Extensive homology between the leukotoxin of Pasteurella haemolytica A1 and the alpha-hemolysin of Escherichia coli. Infect Immun. 1987 Dec;55(12):3233–3236. doi: 10.1128/iai.55.12.3233-3236.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yates W. D. A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. Can J Comp Med. 1982 Jul;46(3):225–263. [PMC free article] [PubMed] [Google Scholar]

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