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. 1991 Feb;59(2):712–715. doi: 10.1128/iai.59.2.712-715.1991

Four epitopes of Pseudomonas aeruginosa elastase defined by monoclonal antibodies.

J Lagacé 1, M Fréchette 1
PMCID: PMC257817  PMID: 1702768

Abstract

Monoclonal antibodies against the elastase of Pseudomonas aeruginosa were produced from spleen cells of BALB/c mice primed with purified elastase of P. aeruginosa and P3-X63-Ag8-U1 myeloma cells. The six clones established generated antibodies which reacted with a 33,000-Da peptide and recognized four different elastase epitopes by a competitive binding enzyme-linked immunosorbent assay. The monoclonal antibodies designated as ELA-17 and ELA-42 that recognize two different epitopes reacted by dot-enzyme immunoassay and by Western immunoblotting with all clinical and International Antigen Typing Scheme strains of P. aeruginosa positive for elastase.

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Selected References

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