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. 1998 May-Aug;71(3-4):163–171.

Physiology of the ECL cells.

R Håkanson 1, D Chen 1, E Lindström 1, P Norlén 1, M Björkqvist 1, D Lehto-Axtelius 1
PMCID: PMC2578978  PMID: 10461349

Abstract

The enterochromaffin-like (ECL) cells of the oxyntic mucosa (fundus) of the stomach produce, store and secrete histamine, chromogranin A-derived peptides such as pancreastatin, and an unanticipated but as yet unidentified peptide hormone. The cells are stimulated by gastrin and pituitary adenylate cyclase activating peptide and suppressed by somatostatin and galanin. Choline esters and histamine seem to be without effect on ECL cell secretion. The existence of a gastrin-ECL cell axis not only explains how gastrin stimulates acid secretion but also may help to explore the functional significance of the ECL cells with respect to the nature and bioactivity of its peptide hormone. From the results of studies of gastrectomized/fundectomized and gastrin-treated rats, it has been speculated that the anticipated ECL-cell peptide hormone acts on bone metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alumets J., Ekelund M., El Munshid H. A., Håkanson R., Lorén I., Sundler F. Topography of somatostatin cells in the stomach of the rat: possible functional significance. Cell Tissue Res. 1979 Nov;202(2):177–188. doi: 10.1007/BF00232233. [DOI] [PubMed] [Google Scholar]
  2. Alumets J., Sundler F., Håkanson R. Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut. Cell Tissue Res. 1977 Dec 28;185(4):465–479. doi: 10.1007/BF00220651. [DOI] [PubMed] [Google Scholar]
  3. Andersson K., Cabero J. L., Mattsson H., Håkanson R. Gastric acid secretion after depletion of enterochromaffin-like cell histamine. A study with alpha-fluoromethylhistidine in rats. Scand J Gastroenterol. 1996 Jan;31(1):24–30. doi: 10.3109/00365529609031622. [DOI] [PubMed] [Google Scholar]
  4. Axelson J., Ekelund M., Håkanson R., Sundler F. Gastrin and the vagus interact in the trophic control of the rat oxyntic mucosa. Regul Pept. 1988 Aug;22(3):237–243. doi: 10.1016/0167-0115(88)90036-5. [DOI] [PubMed] [Google Scholar]
  5. Axelson J., Persson P., Gagnemo-Persson R., Håkanson R. Importance of the stomach in maintaining calcium homoeostasis in the rat. Gut. 1991 Nov;32(11):1298–1302. doi: 10.1136/gut.32.11.1298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Böttcher G., Håkanson R., Nilsson G., Seensalu R., Sundler F. Effects of long-term hypergastrinaemia on the ultrastructure of enterochromaffin-like cells in the stomach of the rat, hamster and guinea pig. Cell Tissue Res. 1989;256(2):247–257. doi: 10.1007/BF00218882. [DOI] [PubMed] [Google Scholar]
  7. Capella C., Vassallo G., Solcia E. Light and electron microscopic identification of the histamine-storing argyrophil (ECL) cell in murine stomach and of its equivalent in other mammals. Z Zellforsch Mikrosk Anat. 1971;118(1):68–84. doi: 10.1007/BF00331767. [DOI] [PubMed] [Google Scholar]
  8. Chen D., Monstein H. J., Nylander A. G., Zhao C. M., Sundler F., Håkanson R. Acute responses of rat stomach enterochromaffinlike cells to gastrin: secretory activation and adaptation. Gastroenterology. 1994 Jul;107(1):18–27. doi: 10.1016/0016-5085(94)90056-6. [DOI] [PubMed] [Google Scholar]
  9. Chen D., Zhao C. M., Andersson K., Meister B., Panula P., Håkanson R. ECL cell morphology. Yale J Biol Med. 1998 May-Aug;71(3-4):217–231. [PMC free article] [PubMed] [Google Scholar]
  10. Ekblad E., Ekelund M., Graffner H., Håkanson R., Sundler F. Peptide-containing nerve fibers in the stomach wall of rat and mouse. Gastroenterology. 1985 Jul;89(1):73–85. doi: 10.1016/0016-5085(85)90747-4. [DOI] [PubMed] [Google Scholar]
  11. Ekblad E., Rökaeus A., Håkanson R., Sundler F. Galanin nerve fibers in the rat gut: distribution, origin and projections. Neuroscience. 1985 Oct;16(2):355–363. doi: 10.1016/0306-4522(85)90008-9. [DOI] [PubMed] [Google Scholar]
  12. Hakanson R., Gagnemo-Persson R., Surve V. V. Perturbations in blood Ca(2)+ do not affect the activity of rat stomach enterochromaffin-like cells. Scand J Gastroenterol. 1996 Mar;31(3):217–221. doi: 10.3109/00365529609004869. [DOI] [PubMed] [Google Scholar]
  13. Håkanson R., Axelson J., Tielemans Y., Johansson A. G., Willems G., Sundler F. Unilateral vagal denervation suppresses omeprazole-induced trophic effects on the denervated side of the rat stomach. Scand J Gastroenterol. 1992;27(1):65–70. doi: 10.3109/00365529209011169. [DOI] [PubMed] [Google Scholar]
  14. Håkanson R., Böttcher G., Ekblad E., Panula P., Simonsson M., Dohlsten M., Hallberg T., Sundler F. Histamine in endocrine cells in the stomach. A survey of several species using a panel of histamine antibodies. Histochemistry. 1986;86(1):5–17. doi: 10.1007/BF00492340. [DOI] [PubMed] [Google Scholar]
  15. Håkanson R., Chen D., Andersson K., Monstein H. J., Zhao C. M., Ryberg B., Sundler F., Mattsson H. The biology and physiology of the ECL cell. Yale J Biol Med. 1994 May-Aug;67(3-4):123–134. [PMC free article] [PubMed] [Google Scholar]
  16. Håkanson R., Ding X. Q., Norlén P., Chen D. Circulating pancreastatin is a marker for the enterochromaffin-like cells of the rat stomach. Gastroenterology. 1995 May;108(5):1445–1452. doi: 10.1016/0016-5085(95)90693-2. [DOI] [PubMed] [Google Scholar]
  17. Håkanson R., Larsson L. I., Liedberg G., Rehfeld J. F., Sundler F. Suppression of rat stomach histidine decarboxylase activity by histamine: H2-receptor-mediated feed-back. J Physiol. 1977 Aug;269(3):643–667. doi: 10.1113/jphysiol.1977.sp011920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Håkanson R., Larsson L. I., Liedberg G., Sundler F. Evidence for H2-receptor-mediated feed-back regulation of histamine release from endocrine cells in the rat stomach. J Physiol. 1978 Mar;276:151–157. doi: 10.1113/jphysiol.1978.sp012224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Håkanson R., Owman C., Sporrong B., Sundler F. Electron microscopic identification of the histamine-storing argyrophil (enterochromaffin-like) cells in the rat stomach. Z Zellforsch Mikrosk Anat. 1971;122(4):460–466. doi: 10.1007/BF00936080. [DOI] [PubMed] [Google Scholar]
  20. Håkanson R., Persson P., Axelson J. Elevated serum gastrin after food intake or acid blockade evokes hypocalcemia. Regul Pept. 1990 Apr 24;28(2):131–136. doi: 10.1016/0167-0115(90)90012-l. [DOI] [PubMed] [Google Scholar]
  21. Håkanson R., Persson P., Axelson J., Johnell O., Sundler F. Evidence that gastrin enhances 45Ca uptake into bone through release of a gastric hormone. Regul Pept. 1990 Mar 27;28(1):107–118. doi: 10.1016/0167-0115(90)90068-8. [DOI] [PubMed] [Google Scholar]
  22. Håkanson R., Sundler F. Proposed mechanism of induction of gastric carcinoids: the gastrin hypothesis. Eur J Clin Invest. 1990 Oct;20 (Suppl 1):S65–S71. doi: 10.1111/j.1365-2362.1990.tb01780.x. [DOI] [PubMed] [Google Scholar]
  23. Håkanson R., Vallgren S., Ekelund M., Rehfeld J. F., Sundler F. The vagus exerts trophic control of the stomach in the rat. Gastroenterology. 1984 Jan;86(1):28–32. [PubMed] [Google Scholar]
  24. Kaplan E. L., North P. T., Norberg H. P., Schulak J. A., Hill B. J. Evidence for a role of the stomach in serum calcium regulation. J Surg Res. 1977 Mar;22(3):237–241. doi: 10.1016/0022-4804(77)90140-8. [DOI] [PubMed] [Google Scholar]
  25. Kimura K., Chen D., Lindström E., Zhao C. M., Håkanson R. Evidence that rat stomach ECL cells represent the main source of circulating pancreastatin. Regul Pept. 1997 Feb 26;68(3):177–180. doi: 10.1016/s0167-0115(96)02117-9. [DOI] [PubMed] [Google Scholar]
  26. Klinge B., Lehto-Axtelius D., Akerman M., Håkanson R. Structure of calvaria after gastrectomy. An experimental study in the rat. Scand J Gastroenterol. 1995 Oct;30(10):952–957. doi: 10.3109/00365529509096337. [DOI] [PubMed] [Google Scholar]
  27. Lehto-Axtelius D., Stenström M., Johnell O. Osteopenia after gastrectomy, fundectomy or antrectomy: an experimental study in the rat. Regul Pept. 1998 Nov 30;78(1-3):41–50. doi: 10.1016/s0167-0115(98)00101-3. [DOI] [PubMed] [Google Scholar]
  28. Lindström E., Björkqvist M., Boketoft A., Chen D., Zhao C. M., Kimura K., Håkanson R. Neurohormonal regulation of histamine and pancreastatin secretion from isolated rat stomach ECL cells. Regul Pept. 1997 Aug 15;71(2):73–86. doi: 10.1016/s0167-0115(97)01018-5. [DOI] [PubMed] [Google Scholar]
  29. Mühlbauer R. C., Schenk R. K., Chen D., Lehto-Axtelius D., Hâkanson R. Morphometric analysis of gastrectomy-evoked osteopenia. Calcif Tissue Int. 1998 Apr;62(4):323–326. doi: 10.1007/s002239900439. [DOI] [PubMed] [Google Scholar]
  30. Norlén P., Curry W. J., Chen D., Zhao C. M., Johnston C. F., Håkanson R. Expression of the chromogranin A-derived peptides pancreastatin and WE14 in rat stomach ECL cells. Regul Pept. 1997 Jun 18;70(2-3):121–133. doi: 10.1016/s0167-0115(97)00021-9. [DOI] [PubMed] [Google Scholar]
  31. Persson P., Gagnemo-Persson R., Chen D., Axelson J., Nylander A. G., Johnell O., Häkanson R. Gastrectomy causes bone loss in the rat: is lack of gastric acid responsible? Scand J Gastroenterol. 1993 Apr;28(4):301–306. doi: 10.3109/00365529309090245. [DOI] [PubMed] [Google Scholar]
  32. Persson P., Håkanson R., Axelson J., Sundler F. Gastrin releases a blood calcium-lowering peptide from the acid-producing part of the rat stomach. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2834–2838. doi: 10.1073/pnas.86.8.2834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Prinz C., Kajimura M., Scott D. R., Mercier F., Helander H. F., Sachs G. Histamine secretion from rat enterochromaffinlike cells. Gastroenterology. 1993 Aug;105(2):449–461. doi: 10.1016/0016-5085(93)90719-s. [DOI] [PubMed] [Google Scholar]
  34. Prinz C., Scott D. R., Hurwitz D., Helander H. F., Sachs G. Gastrin effects on isolated rat enterochromaffin-like cells in primary culture. Am J Physiol. 1994 Oct;267(4 Pt 1):G663–G675. doi: 10.1152/ajpgi.1994.267.4.G663. [DOI] [PubMed] [Google Scholar]
  35. Schulak J. A., Kaplan E. L. Gastrin-induced hypocalcemia in thyroparathyroidectomized rats. Metabolism. 1974 Dec;23(12):1103–1106. doi: 10.1016/0026-0495(74)90026-2. [DOI] [PubMed] [Google Scholar]
  36. Schulak J. A., Kaplan E. L. The importance of the stomach in gastrin-induced hypocalcemia in the rat. Endocrinology. 1975 May;96(5):1217–1220. doi: 10.1210/endo-96-5-1217. [DOI] [PubMed] [Google Scholar]
  37. Stenström M., Olander B., Carlsson C. A., Carlsson G. A., Håkanson R. Methodologic aspects of computed microtomography to monitor the development of osteoporosis in gastrectomized rats. Acad Radiol. 1995 Sep;2(9):785–791. doi: 10.1016/s1076-6332(05)80487-8. [DOI] [PubMed] [Google Scholar]
  38. Sundler F., Ekblad E., Absood A., Håkanson R., Köves K., Arimura A. Pituitary adenylate cyclase activating peptide: a novel vasoactive intestinal peptide-like neuropeptide in the gut. Neuroscience. 1992;46(2):439–454. doi: 10.1016/0306-4522(92)90064-9. [DOI] [PubMed] [Google Scholar]
  39. Sundler F., Håkanson R., Lorén I., Lundquist I. Amine storage and function in peptide hormone-producing cells. Invest Cell Pathol. 1980 Jan-Mar;3(1):87–103. [PubMed] [Google Scholar]
  40. Waldum H. L., Sandvik A. K., Brenna E., Petersen H. Gastrin-histamine sequence in the regulation of gastric acid secretion. Gut. 1991 Jun;32(6):698–701. doi: 10.1136/gut.32.6.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Zhao C. M., Jacobsson G., Chen D., Hâkanson R., Meister B. Exocytotic proteins in enterochromaffin-like (ECL) cells of the rat stomach. Cell Tissue Res. 1997 Dec;290(3):539–551. doi: 10.1007/s004410050960. [DOI] [PubMed] [Google Scholar]

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