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. 1991 Jun;59(6):2006–2011. doi: 10.1128/iai.59.6.2006-2011.1991

Surface exposure of O1 serotype lipopolysaccharide in Klebsiella pneumoniae strains expressing different K antigens.

J M Tomás 1, S Camprubi 1, S Merino 1, M R Davey 1, P Williams 1
PMCID: PMC257957  PMID: 1709919

Abstract

Surface exposure of the O1 serotype lipopolysaccharide in encapsulated Klebsiella pneumoniae strains belonging to different serotypes was examined by using the O1 antigen-specific bacteriophages FC3-1 and FC3-2 in conjunction with immunogold electron microscopy and enzyme immunoassays with specific antisera. Despite the presence of the capsular polysaccharide, the O1 antigen was exposed at the cell surface in strains producing K2, K7, K8, K12, K19, K21, K22, K34, K35, K42, K45, K55, K57, K62, K66, K69, and K70 capsular polysaccharides. However, in strains producing K1, K10, and K16 capsular polysaccharides, the O1 antigen was masked by the K antigen. These results suggest that, since the O1 antigen is surface exposed in many different strains of K. pneumoniae with different capsular serotypes and is also able to immunoprotect, its potential as a useful vaccine component should not be overlooked.

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Selected References

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  1. Ames G. F., Spudich E. N., Nikaido H. Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations. J Bacteriol. 1974 Feb;117(2):406–416. doi: 10.1128/jb.117.2.406-416.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bryan C. S., Reynolds K. L., Brenner E. R. Analysis of 1,186 episodes of gram-negative bacteremia in non-university hospitals: the effects of antimicrobial therapy. Rev Infect Dis. 1983 Jul-Aug;5(4):629–638. doi: 10.1093/clinids/5.4.629. [DOI] [PubMed] [Google Scholar]
  3. Ciurana B., Tomás J. M. Role of lipopolysaccharide and complement in susceptibility of Klebsiella pneumoniae to nonimmune serum. Infect Immun. 1987 Nov;55(11):2741–2746. doi: 10.1128/iai.55.11.2741-2746.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cryz S. J., Jr, Fürer E., Germanier R. Immunization against fatal experimental Klebsiella pneumoniae pneumonia. Infect Immun. 1986 Nov;54(2):403–407. doi: 10.1128/iai.54.2.403-407.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cryz S. J., Jr, Mortimer P. M., Mansfield V., Germanier R. Seroepidemiology of Klebsiella bacteremic isolates and implications for vaccine development. J Clin Microbiol. 1986 Apr;23(4):687–690. doi: 10.1128/jcm.23.4.687-690.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cryz S. J. Progress in immunization against Klebsiella infections. Eur J Clin Microbiol. 1983 Dec;2(6):523–528. doi: 10.1007/BF02016559. [DOI] [PubMed] [Google Scholar]
  7. Domenico P., Diedrich D. L., Straus D. C. Extracellular polysaccharide production by Klebsiella pneumoniae and its relationship to virulence. Can J Microbiol. 1985 May;31(5):472–478. doi: 10.1139/m85-088. [DOI] [PubMed] [Google Scholar]
  8. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973 Sep;115(3):717–722. doi: 10.1128/jb.115.3.717-722.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Godfrey A. J., Shahrabadi M. S., Bryan L. E. Distribution of porin and lipopolysaccharide antigens on a Pseudomonas aeruginosa permeability mutant. Antimicrob Agents Chemother. 1986 Nov;30(5):802–805. doi: 10.1128/aac.30.5.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jarvis W. R., Munn V. P., Highsmith A. K., Culver D. H., Hughes J. M. The epidemiology of nosocomial infections caused by Klebsiella pneumoniae. Infect Control. 1985 Feb;6(2):68–74. doi: 10.1017/s0195941700062639. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Mizuta K., Ohta M., Mori M., Hasegawa T., Nakashima I., Kato N. Virulence for mice of Klebsiella strains belonging to the O1 group: relationship to their capsular (K) types. Infect Immun. 1983 Apr;40(1):56–61. doi: 10.1128/iai.40.1.56-61.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robinson E. N., Jr, McGee Z. A., Kaplan J., Hammond M. E., Larson J. K., Buchanan T. M., Schoolnik G. K. Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes. Infect Immun. 1984 Nov;46(2):361–366. doi: 10.1128/iai.46.2.361-366.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Simoons-Smit A. M., Verweij-van Vught A. M., MacLaren D. M. The role of K antigens as virulence factors in Klebsiella. J Med Microbiol. 1986 Mar;21(2):133–137. doi: 10.1099/00222615-21-2-133. [DOI] [PubMed] [Google Scholar]
  15. Tomas J. M., Camprubi S., Williams P. Surface exposure of the O-antigen in Klebsiella pneumoniae O1:K1 serotype strains. Microb Pathog. 1988 Aug;5(2):141–147. doi: 10.1016/0882-4010(88)90016-2. [DOI] [PubMed] [Google Scholar]
  16. Tomás J. M., Benedí V. J., Ciurana B., Jofre J. Role of capsule and O antigen in resistance of Klebsiella pneumoniae to serum bactericidal activity. Infect Immun. 1986 Oct;54(1):85–89. doi: 10.1128/iai.54.1.85-89.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tomás J. M., Jofre J. T. Lipopolysaccharide-specific bacteriophage for Klebsiella pneumoniae C3. J Bacteriol. 1985 Jun;162(3):1276–1279. doi: 10.1128/jb.162.3.1276-1279.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  19. Williams P., Lambert P. A., Brown M. R., Jones R. J. The role of the O and K antigens in determining the resistance of Klebsiella aerogenes to serum killing and phagocytosis. J Gen Microbiol. 1983 Jul;129(7):2181–2191. doi: 10.1099/00221287-129-7-2181. [DOI] [PubMed] [Google Scholar]
  20. Williams P., Lambert P. A., Haigh C. G., Brown M. R. The influence of the O and K antigens of Klebsiella aerogenes on surface hydrophobicity and susceptibility to phagocytosis and antimicrobial agents. J Med Microbiol. 1986 Mar;21(2):125–132. doi: 10.1099/00222615-21-2-125. [DOI] [PubMed] [Google Scholar]

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