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. 1991 Jun;59(6):2116–2119. doi: 10.1128/iai.59.6.2116-2119.1991

Gastrointestinal candidiasis in a murine model of severe combined immunodeficiency syndrome.

R Narayanan 1, W A Joyce 1, R A Greenfield 1
PMCID: PMC257974  PMID: 2037373

Abstract

A murine model of severe combined immunodeficiency syndrome (scid mice) affords an opportunity to study the interaction of Candida albicans with a host lacking functional B- and T-cell mechanisms. We have previously reported no significant difference in yeast recovery after intravenous challenge of BALB/c mice and scid mice with C. albicans (S. Mahanty, R.A. Greenfield, W.A. Joyce, and P.W. Kincade, Infect. Immun. 56:3162-3166, 1988). In this study, we evaluate the course of gastrointestinal candidiasis after a single oral challenge with C. albicans. BALB/c and scid mice received H2O containing 10(6) C. albicans per ml for 16 h. Half the mice of each strain continuously received H2O containing 1 mg of tetracycline per ml. Stool samples were cultured for yeast twice weekly until they were negative three consecutive times or positive for 8 weeks. Mice were then sacrificed for quantitative cultures of liver, spleen, and kidneys. At eight weeks postinoculation, 2 of 13 BALB/c mice, 0 of 14 BALB/c mice receiving tetracycline, 6 of 12 scid mice, and 8 of 13 scid mice receiving tetracycline had positive stool cultures (P less than 0.05, likelihood ratio chi-square). Quantitative recovery of yeasts from stools was also higher in the scid mice. Cultures of liver, spleen, and kidneys wer negative in all BALB/c mice and essentially all negative in scid mice; a single colony was isolated from the kidney of one scid mouse and the liver of another scid mouse. We conclude that B cells and/or T cells and their products are important in gastrointestinal colonization with C. albicans but that even in their absence, dissemination of infection from the gastrointestinal tract does not consistently occur. Thus, other aspects of host defense must be critical in containing gastrointestinal Candida colonization.

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Selected References

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  1. Balish E., Filutowicz H., Oberley T. D. Correlates of cell-mediated immunity in Candida albicans-colonized gnotobiotic mice. Infect Immun. 1990 Jan;58(1):107–113. doi: 10.1128/iai.58.1.107-113.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bosma G. C., Custer R. P., Bosma M. J. A severe combined immunodeficiency mutation in the mouse. Nature. 1983 Feb 10;301(5900):527–530. doi: 10.1038/301527a0. [DOI] [PubMed] [Google Scholar]
  3. Bowen D. L., Lane H. C., Fauci A. S. Immunopathogenesis of the acquired immunodeficiency syndrome. Ann Intern Med. 1985 Nov;103(5):704–709. doi: 10.7326/0003-4819-103-5-704. [DOI] [PubMed] [Google Scholar]
  4. Cantorna M. T., Balish E. Mucosal and systemic candidiasis in congenitally immunodeficient mice. Infect Immun. 1990 Apr;58(4):1093–1100. doi: 10.1128/iai.58.4.1093-1100.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crislip M. A., Edwards J. E., Jr Candidiasis. Infect Dis Clin North Am. 1989 Mar;3(1):103–133. [PubMed] [Google Scholar]
  6. Custer R. P., Bosma G. C., Bosma M. J. Severe combined immunodeficiency (SCID) in the mouse. Pathology, reconstitution, neoplasms. Am J Pathol. 1985 Sep;120(3):464–477. [PMC free article] [PubMed] [Google Scholar]
  7. DeGregorio M. W., Lee W. M., Ries C. A. Candida infections in patients with acute leukemia: ineffectiveness of nystatin prophylaxis and relationship between oropharyngeal and systemic candidiasis. Cancer. 1982 Dec 15;50(12):2780–2784. doi: 10.1002/1097-0142(19821215)50:12<2780::aid-cncr2820501215>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  8. Domer J. E. Intragastric colonization of infant mice with Candida albicans induces systemic immunity demonstrable upon challenge as adults. J Infect Dis. 1988 May;157(5):950–958. doi: 10.1093/infdis/157.5.950. [DOI] [PubMed] [Google Scholar]
  9. Dorshkind K., Keller G. M., Phillips R. A., Miller R. G., Bosma G. C., O'Toole M., Bosma M. J. Functional status of cells from lymphoid and myeloid tissues in mice with severe combined immunodeficiency disease. J Immunol. 1984 Apr;132(4):1804–1808. [PubMed] [Google Scholar]
  10. Ekenna O., Sherertz R. J. Factors affecting colonization and dissemination of Candida albicans from the gastrointestinal tract of mice. Infect Immun. 1987 Jul;55(7):1558–1563. doi: 10.1128/iai.55.7.1558-1563.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Helstrom P. B., Balish E. Effect of oral tetracycline, the microbial flora, and the athymic state on gastrointestinal colonization and infection of BALB/c mice with Candida albicans. Infect Immun. 1979 Mar;23(3):764–774. doi: 10.1128/iai.23.3.764-774.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kennedy M. J. Inhibition of Candida albicans by the anaerobic oral flora of mice in vitro. Sabouraudia. 1981 Sep;19(3):205–208. [PubMed] [Google Scholar]
  13. Kennedy M. J., Volz P. A. Ecology of Candida albicans gut colonization: inhibition of Candida adhesion, colonization, and dissemination from the gastrointestinal tract by bacterial antagonism. Infect Immun. 1985 Sep;49(3):654–663. doi: 10.1128/iai.49.3.654-663.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kumar V., Hackett J., Jr, Tutt M. M., Garni-Wagner B. A., Kuziel W. A., Tucker P. W., Bennett M. Natural killer cells and their precursors in mice with severe combined immunodeficiency. Curr Top Microbiol Immunol. 1989;152:47–52. doi: 10.1007/978-3-642-74974-2_7. [DOI] [PubMed] [Google Scholar]
  15. Mahanty S., Greenfield R. A., Joyce W. A., Kincade P. W. Inoculation candidiasis in a murine model of severe combined immunodeficiency syndrome. Infect Immun. 1988 Dec;56(12):3162–3166. doi: 10.1128/iai.56.12.3162-3166.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Matthews R., Burnie J., Smith D., Clark I., Midgley J., Conolly M., Gazzard B. Candida and AIDS: evidence for protective antibody. Lancet. 1988 Jul 30;2(8605):263–266. doi: 10.1016/s0140-6736(88)92547-0. [DOI] [PubMed] [Google Scholar]
  17. Schuler W., Bosma M. J. Nature of the scid defect: a defective VDJ recombinase system. Curr Top Microbiol Immunol. 1989;152:55–62. doi: 10.1007/978-3-642-74974-2_8. [DOI] [PubMed] [Google Scholar]
  18. Schuler W., Weiler I. J., Schuler A., Phillips R. A., Rosenberg N., Mak T. W., Kearney J. F., Perry R. P., Bosma M. J. Rearrangement of antigen receptor genes is defective in mice with severe combined immune deficiency. Cell. 1986 Sep 26;46(7):963–972. doi: 10.1016/0092-8674(86)90695-1. [DOI] [PubMed] [Google Scholar]
  19. Shultz L. D., Sidman C. L. Genetically determined murine models of immunodeficiency. Annu Rev Immunol. 1987;5:367–403. doi: 10.1146/annurev.iy.05.040187.002055. [DOI] [PubMed] [Google Scholar]

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