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. 1991 Jun;59(6):2200–2202. doi: 10.1128/iai.59.6.2200-2202.1991

Detection of immunoglobulin G antibodies to cholesterol in antisera to mycoplasmas.

T Watanabe 1, T Kumazawa 1
PMCID: PMC257989  PMID: 2037380

Abstract

Antisera were produced by inoculation of mycoplasma cells grown in PPLO broth supplemented with rabbit serum alone or rabbit serum plus Freund complete or incomplete adjuvant. By using an enzyme-linked immunosorbent assay, immunoglobulin G antibodies to cholesterol were detected in antisera to mycoplasmas (Mycoplasma salivarium ATCC 23064, M. orale ATCC 15539, M. buccale IID 802, M. faucium IID 996, and M. hominis IID 801) and rabbit serum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradbury J. M., Jordan F. T. Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions. J Hyg (Lond) 1972 Jun;70(2):267–278. doi: 10.1017/s0022172400022324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eschenbach D. A., Buchanan T. M., Pollock H. M., Forsyth P. S., Alexander E. R., Lin J. S., Wang S. P., Wentworth B. B., MacCormack W. M., Holmes K. K. Polymicrobial etiology of acute pelvic inflammatory disease. N Engl J Med. 1975 Jul 24;293(4):166–171. doi: 10.1056/NEJM197507242930403. [DOI] [PubMed] [Google Scholar]
  3. Hammerschmidt D. E., Greenberg C. S., Yamada O., Craddock P. R., Jacob H. S. Cholesterol and atheroma lipids activate complement and stimulate granulocytes. A possible mechanism for amplification of ischemic injury in atherosclerotic states. J Lab Clin Med. 1981 Jul;98(1):68–77. [PubMed] [Google Scholar]
  4. Harwick H. J., Purcell R. H., Iuppa J. B., Fekety F. R., Jr Mycoplasma hominis and abortion. J Infect Dis. 1970 Mar;121(3):260–268. doi: 10.1093/infdis/121.3.260. [DOI] [PubMed] [Google Scholar]
  5. Loizou S., McCrea J. D., Rudge A. C., Reynolds R., Boyle C. C., Harris E. N. Measurement of anti-cardiolipin antibodies by an enzyme-linked immunosorbent assay (ELISA): standardization and quantitation of results. Clin Exp Immunol. 1985 Dec;62(3):738–745. [PMC free article] [PubMed] [Google Scholar]
  6. McCormack W. M., Braun P., Lee Y. H., Klein J. O., Kass E. H. The genital mycoplasmas. N Engl J Med. 1973 Jan 11;288(2):78–89. doi: 10.1056/NEJM197301112880206. [DOI] [PubMed] [Google Scholar]
  7. SMITH P. F. Fate of ergosterol and cholestanol in pleuro-pneumonia-like organisms. J Bacteriol. 1962 Sep;84:534–538. doi: 10.1128/jb.84.3.534-538.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sugiyama T. Medium components adsorbed to mycoplasmal cells. Yale J Biol Med. 1983 Sep-Dec;56(5-6):691–693. [PMC free article] [PubMed] [Google Scholar]
  9. Swartz G. M., Jr, Gentry M. K., Amende L. M., Blanchette-Mackie E. J., Alving C. R. Antibodies to cholesterol. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1902–1906. doi: 10.1073/pnas.85.6.1902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Watanabe T., Matsuura M., Seto K. Enumeration, isolation, and species identification of mycoplasmas in saliva sampled from the normal and pathological human oral cavity and antibody response to an oral mycoplasma (Mycoplasma salivarium). J Clin Microbiol. 1986 Jun;23(6):1034–1038. doi: 10.1128/jcm.23.6.1034-1038.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Watanabe T., Shibata K., Hasegawa K. Production of immunoglobulin M (IgM) autoantibodies to IgG in rabbits inoculated with Mycoplasma salivarium cells grown in medium supplemented with rabbit serum. Infect Immun. 1987 Jan;55(1):263–265. doi: 10.1128/iai.55.1.263-265.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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