Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1991 Aug;59(8):2799–2805. doi: 10.1128/iai.59.8.2799-2805.1991

Analysis of immune responses of different hosts to Babesia divergens isolates from different geographic areas and capacity of culture-derived exoantigens to induce efficient cross-protection.

E Precigout 1, A Gorenflot 1, A Valentin 1, G Bissuel 1, B Carcy 1, P Brasseur 1, Y Moreau 1, J Schrevel 1
PMCID: PMC258089  PMID: 1713201

Abstract

The immunoprecipitation of [35S]methionine-radiolabelled antigens from different Babesia divergens isolates by using bovine, gerbil, and human immune sera has shown that many B. divergens proteins contain epitopes shared between isolates. The cross-protective capacity of culture-derived soluble immunogens from the B. divergens Rouen 1987 isolate was tested against different B. divergens isolates. Results showed complete protection against the 7107b French isolate and substantial protection against the Weybridge 8843 English isolate (80% protection) and the Munich 87 German isolate (60% protection). In order to explain these vaccination results and to assess both the common and variable antigenicity of B. divergens, the antigenic patterns of the challenge isolates (Rouen 1987, 7107b, Weybridge 8843, and Munich 87) were compared by immunoprecipitation, using gerbil antisera raised against the Rouen 1987 vaccine isolate. Differences in the antigenic patterns and in the cross-protection of gerbils in these heterologous challenges were examined by studying the virulence and the antigenic status of each isolate.

Full text

PDF
2799

Images in this article

2800Image
on p.2800
2801Image
on p.2801
2802Image
on p.2802
2802Image
on p.2802
2803Image
on p.2803

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anders R. F. Multiple cross-reactivities amongst antigens of Plasmodium falciparum impair the development of protective immunity against malaria. Parasite Immunol. 1986 Nov;8(6):529–539. doi: 10.1111/j.1365-3024.1986.tb00867.x. [DOI] [PubMed] [Google Scholar]
  2. Bordier C. Phase separation of integral membrane proteins in Triton X-114 solution. J Biol Chem. 1981 Feb 25;256(4):1604–1607. [PubMed] [Google Scholar]
  3. Cox F. E., Young A. S. Acquired immunity to Babesia microti and Babesia rodhaini in mice. Parasitology. 1969 Feb;59(1):257–268. doi: 10.1017/s0031182000069997. [DOI] [PubMed] [Google Scholar]
  4. Dalgliesh R. J., Stewart N. P. Tolerance to imidocarb induced experimentally in tick-transmitted Babesia argentina. Aust Vet J. 1977 Apr;53(4):176–180. doi: 10.1111/j.1751-0813.1977.tb00163.x. [DOI] [PubMed] [Google Scholar]
  5. Englund P. T., Hajduk S. L., Marini J. C. The molecular biology of trypanosomes. Annu Rev Biochem. 1982;51:695–726. doi: 10.1146/annurev.bi.51.070182.003403. [DOI] [PubMed] [Google Scholar]
  6. Figueroa J. V., Buening G. M., Kinden D. A., Green T. J. Identification of common surface antigens among Babesia bigemina isolates by using monoclonal antibodies. Parasitology. 1990 Apr;100(Pt 2):161–175. doi: 10.1017/s0031182000061163. [DOI] [PubMed] [Google Scholar]
  7. Gorenflot A., Brasseur P., Bonmarchand G., Laneele D., Simonin D. Deux cas de babédiose humaine grave traitée avec succès. Presse Med. 1990 Feb 24;19(7):335–335. [PubMed] [Google Scholar]
  8. Gorenflot A., Brasseur P., Precigout E., L'Hostis M., Marchand A., Schrevel J. Cytological and immunological responses to Babesia divergens in different hosts: ox, gerbil, man. Parasitol Res. 1991;77(1):3–12. doi: 10.1007/BF00934377. [DOI] [PubMed] [Google Scholar]
  9. Gorenflot A., Precigout E., Bissuel G., Lecointre O., Brasseur P., Vidor E., L'Hostis M., Schrevel J. Identification of major Babesia divergens polypeptides that induce protection against homologous challenge in gerbils. Infect Immun. 1990 Dec;58(12):4076–4082. doi: 10.1128/iai.58.12.4076-4082.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kahl L. P., Anders R. F., Rodwell B. J., Timms P., Mitchell G. F. Variable and common antigens of Babesia bovis parasites differing in strain and virulence. J Immunol. 1982 Oct;129(4):1700–1705. [PubMed] [Google Scholar]
  11. Lewis D., Williams H. Infection of the Mongolian gerbil with the cattle piroplasm Babesia divergens. Nature. 1979 Mar 8;278(5700):170–171. doi: 10.1038/278170a0. [DOI] [PubMed] [Google Scholar]
  12. McElwain T. F., Palmer G. H., Goff W. L., McGuire T. C. Identification of Babesia bigemina and Babesia bovis merozoite proteins with isolate- and species-common epitopes recognized by antibodies in bovine immune sera. Infect Immun. 1988 Jun;56(6):1658–1660. doi: 10.1128/iai.56.6.1658-1660.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McElwain T. F., Perryman L. E., Davis W. C., McGuire T. C. Antibodies define multiple proteins with epitopes exposed on the surface of live Babesia bigemina merozoites. J Immunol. 1987 Apr 1;138(7):2298–2304. [PubMed] [Google Scholar]
  14. Montenegro-James S., Ristic M., Toro Benitez M., Leon E., Lopez R. Heterologous strain immunity in bovine babesiosis using a culture-derived soluble Babesia bovis immunogen. Vet Parasitol. 1985 Dec;18(4):321–337. doi: 10.1016/0304-4017(85)90067-6. [DOI] [PubMed] [Google Scholar]
  15. Montenegro-James S., Toro Benitez M., Leon E., Lopez R., Ristic M. Bovine babesiosis: induction of protective immunity with culture-derived Babesia bovis and Babesia bigemina immunogens. Parasitol Res. 1987;74(2):142–150. doi: 10.1007/BF00536025. [DOI] [PubMed] [Google Scholar]
  16. Phillips R. S., Reid G. M., McLean S. A., Pearson C. D. Antigenic diversity in Babesia divergens: preliminary results with three monoclonal antibodies to the rat-adapted strain. Res Vet Sci. 1987 Jan;42(1):96–100. [PubMed] [Google Scholar]
  17. Raoult D., Soulayrol L., Toga B., Dumon H., Casanova P. Babesiosis, pentamidine, and cotrimoxazole. Ann Intern Med. 1987 Dec;107(6):944–944. doi: 10.7326/0003-4819-107-6-944_2. [DOI] [PubMed] [Google Scholar]
  18. Smith R. D., Molinar E., Larios F., Monroy J., Trigo F., Ristic M. Bovine babesiosis: pathogenicity and heterologous species immunity of tick-borne Babesia bovis and B bigemina infections. Am J Vet Res. 1980 Dec;41(12):1957–1965. [PubMed] [Google Scholar]
  19. Taylor S. M., Elliott C. T., Kenny J. Babesia divergens: sequential exposure to heterologous tick-borne challenge of cattle immunized with a fraction of parasitized erythrocytes. J Comp Pathol. 1986 Jan;96(1):101–107. doi: 10.1016/0021-9975(86)90027-7. [DOI] [PubMed] [Google Scholar]
  20. Tetzlaff C. L., McMurray D. N., Rice-Ficht A. C. Isolation and characterization of a gene associated with a virulent strain of Babesia microti. Mol Biochem Parasitol. 1990 May;40(2):183–192. doi: 10.1016/0166-6851(90)90040-s. [DOI] [PubMed] [Google Scholar]
  21. Tibayrenc M., Kjellberg F., Ayala F. J. A clonal theory of parasitic protozoa: the population structures of Entamoeba, Giardia, Leishmania, Naegleria, Plasmodium, Trichomonas, and Trypanosoma and their medical and taxonomical consequences. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2414–2418. doi: 10.1073/pnas.87.7.2414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Timms P., Dalgliesh R. J., Barry D. N., Dimmock C. K., Rodwell B. J. Babesia bovis: comparison of culture-derived parasites, non-living antigen and conventional vaccine in the protection of cattle against heterologous challenge. Aust Vet J. 1983 Mar;60(3):75–77. doi: 10.1111/j.1751-0813.1983.tb05874.x. [DOI] [PubMed] [Google Scholar]
  23. Timms P. Development of babesial vaccines. Trans R Soc Trop Med Hyg. 1989;83 (Suppl):73–79. doi: 10.1016/0035-9203(89)90608-1. [DOI] [PubMed] [Google Scholar]
  24. Wright I. G., Goodger B. V., Leatch G., Aylward J. H., Rode-Bramanis K., Waltisbuhl D. J. Protection of Babesia bigemina-immune animals against subsequent challenge with virulent Babesia bovis. Infect Immun. 1987 Feb;55(2):364–368. doi: 10.1128/iai.55.2.364-368.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yeruham I., Pipano E., Davidson M. A field strain of Babesia bovis apparently resistant to amicarbalide isethionate. Trop Anim Health Prod. 1985 Feb;17(1):29–30. doi: 10.1007/BF02356129. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES