Abstract
A rat model of congenital toxoplasmosis is described for the study of protective immunity and chemotherapy during pregnancy. Six Sprague-Dawley 7- to 15-day gestational rats were inoculated orally (three rats, trial A) or subcutaneously (three rats, trial B) with 10,000 infective oocysts of the CT-1 strain of Toxoplasma gondii. The tissues of rat pups born from these rats were bioassayed for T. gondii infection. T. gondii was recovered from 30 of 33 (90.9%, trial B) and 23 of 28 (82.17%, trial A) rat pups bioassayed at 1 to 134 days of age. Two 10- to 14-day gestational pregnant rats were inoculated subcutaneously (trial C) with 10,000 infective bradyzoites from tissue cysts; 10 of 23 (43.8%) of the newborn pups were infected with T. gondii. Congenital infection occurred only when rats were infected during pregnancy. None of three rats in trial A that had given birth to congenitally infected rat pups produced congenitally infected rat pups during the second pregnancy.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Araujo F. G., Remington J. S. Partially purified antigen preparations of Toxoplasma gondii protect against lethal infection in mice. Infect Immun. 1984 Jul;45(1):122–126. doi: 10.1128/iai.45.1.122-126.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BEVERLEY J. K. Congenital transmission of toxoplasmosis through successive generations of mice. Nature. 1959 May 9;183(4671):1348–1349. doi: 10.1038/1831348a0. [DOI] [PubMed] [Google Scholar]
- Dubey J. P., Desmonts G. Serological responses of equids fed Toxoplasma gondii oocysts. Equine Vet J. 1987 Jul;19(4):337–339. doi: 10.1111/j.2042-3306.1987.tb01426.x. [DOI] [PubMed] [Google Scholar]
- Dubey J. P. Status of toxoplasmosis in sheep and goats in the United States. J Am Vet Med Assoc. 1990 Jan 15;196(2):259–262. [PubMed] [Google Scholar]
- HELLBRUGGE T. Die fetale Infektion im Verlauf der akuten und chronischen Phase bei der latenten Rattentoxoplasmose. Arch Gynakol. 1955;186:384–388. doi: 10.1007/BF02124596. [DOI] [PubMed] [Google Scholar]
- McLeod R., Frenkel J. K., Estes R. G., Mack D. G., Eisenhauer P. B., Gibori G. Subcutaneous and intestinal vaccination with tachyzoites of Toxoplasma gondii and acquisition of immunity to peroral and congenital toxoplasma challenge. J Immunol. 1988 Mar 1;140(5):1632–1637. [PubMed] [Google Scholar]
- Pettersen E. K. Resistance to avirulent Toxoplasma gondii in normal and vaccinated rats. APMIS. 1988 Sep;96(9):820–824. doi: 10.1111/j.1699-0463.1988.tb00949.x. [DOI] [PubMed] [Google Scholar]
- REMINGTON J. S. Experiments on the transmission of toxoplasmosis. Surv Ophthalmol. 1961 Dec;6:856–876. [PubMed] [Google Scholar]
- Roberts T., Frenkel J. K. Estimating income losses and other preventable costs caused by congenital toxoplasmosis in people in the United States. J Am Vet Med Assoc. 1990 Jan 15;196(2):249–256. [PubMed] [Google Scholar]