Abstract
Contiguous exposed antibody-binding regions of the antigen d flagellin of Salmonella muenchen were identified by using octameric peptides synthesized on polyethylene pins. Identification was confirmed by the serological activity of immunoglobulins recovered from specified pin peptides. Peptides equivalent to four regions of the d flagellin reacted with three different sera tested.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Geysen H. M., Rodda S. J., Mason T. J., Tribbick G., Schoofs P. G. Strategies for epitope analysis using peptide synthesis. J Immunol Methods. 1987 Sep 24;102(2):259–274. doi: 10.1016/0022-1759(87)90085-8. [DOI] [PubMed] [Google Scholar]
- Joys T. M. Identification of an antibody binding site in the phase-1 flagellar protein of Salmonella typhimurium. Microbios. 1976;15(61-62):221–228. [PubMed] [Google Scholar]
- Joys T. M., Stocker B. A. Isolation and serological analysis of mutant forms of flagellar antigen i of Salmonella typhimurium. J Gen Microbiol. 1966 Jul;44(1):121–138. doi: 10.1099/00221287-44-1-121. [DOI] [PubMed] [Google Scholar]
- Joys T. M. The flagellar filament protein. Can J Microbiol. 1988 Apr;34(4):452–458. doi: 10.1139/m88-078. [DOI] [PubMed] [Google Scholar]
- Netter H. J., Guldner H. H., Szostecki C., Lakomek H. J., Will H. A recombinant autoantigen derived from the human (U1) small nuclear RNP-specific 68-kd protein. Expression in Escherichia coli and serodiagnostic application. Arthritis Rheum. 1988 May;31(5):616–622. doi: 10.1002/art.1780310506. [DOI] [PubMed] [Google Scholar]
- Newton S. M., Jacob C. O., Stocker B. A. Immune response to cholera toxin epitope inserted in Salmonella flagellin. Science. 1989 Apr 7;244(4900):70–72. doi: 10.1126/science.2468182. [DOI] [PubMed] [Google Scholar]
- Smith D. E., Fisher P. A. Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots. J Cell Biol. 1984 Jul;99(1 Pt 1):20–28. doi: 10.1083/jcb.99.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wei L. N., Joys T. M. Covalent structure of three phase-1 flagellar filament proteins of Salmonella. J Mol Biol. 1985 Dec 20;186(4):791–803. doi: 10.1016/0022-2836(85)90397-3. [DOI] [PubMed] [Google Scholar]