Figure 8.—

Transmission as a function of the oma-1 genetic background. (A) We examined the transmission of the silencing character in two loss-of-function backgrounds of oma-1. The genetic scheme shows the missense mutation zu405te36 as the starting point for the experiment. The same scheme was followed to examine the silencing efficacy, starting with the loss-of-function nonsense mutation zu405te33. Loss-of-function strains (zu405te36 and zu405te33) of oma-1 were injected with dsRNA trigger A2 and crossed to gain-of-function zu405 strains. Sperm transmission experiments were done at 16° and oocyte transmission at 23°. (B) Calculated frequencies of silencing were measured by the frequency of homozygous zu405 F₃ animals with viable F₄ progeny.