TABLE 2.
Effect of the Scnm1Δ3-5 targeted allele on splicing of the Scn8amedJ transcript
| Genotype | No. of mice | % correctly spliced Scn8a transcripts | Previous estimates (%) |
|---|---|---|---|
| Scn8a+/+ | 3 | 100 ± 16 (12) | 100 by definition |
| Scn8amedJ/medJ, Scnm1+/+ | 1 | 8.1 ± 0.7 (4) | 12a; 10b |
| Scn8amedJ/medJ, ScnmR187X/R187X | 2 | 3.8 ± 0.4 (8) | 6.4a; 5b |
| Scn8amedJ/medJ, Scnm1Δ3-5/R187X | 1 | 3.4 ± 0.3 (4) | NA |
| Scn8amedJ/medJ, Scnm1Δ3-5/Δ3-5 | 2 | 1.4 ± 0.1 (8) | NA |
Mice were generated from intercrosses of (B6.Scn8a1medJ/+, Scnm1Δ3-5/R187X) mice and of (B6.Scn8a1medJ/+, Scnm1R187X/+) mice. Scn8a transcripts in brain RNA were quantitated with a Taqman assay; the probe spanned the exon1/exon 2 junction to specifically detect correctly spliced Scn8a transcripts. Values represent 2(−ΔΔCt) × 100, mean ± SD (n), where n is the number of replicate assays (four per mouse). NA, not applicable.