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. 1992 Dec;60(12):5312–5315. doi: 10.1128/iai.60.12.5312-5315.1992

Encystation of Giardia lamblia leads to expression of antigens recognized by antibodies against conserved heat shock proteins.

D S Reiner 1, T M Shinnick 1, F Ardeshir 1, F D Gillin 1
PMCID: PMC258313  PMID: 1452366

Abstract

During in vitro encystation, Giardia lamblia expresses several stage-specific proteins which are recognized in immunoblots by antisera raised against antigens from three different pathogens. The antigens belong to two different families of conserved stress proteins: (i) HSP60 purified from Legionella pneumophila and recombinant HSP60 from Mycobacterium bovis BCG and (ii) recombinant HSP70 from Plasmodium falciparum.

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Selected References

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  1. Adam R. D., Aggarwal A., Lal A. A., de La Cruz V. F., McCutchan T., Nash T. E. Antigenic variation of a cysteine-rich protein in Giardia lamblia. J Exp Med. 1988 Jan 1;167(1):109–118. doi: 10.1084/jem.167.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aggarwal A., de la Cruz V. F., Nash T. E. A heat shock protein gene in Giardia lamblia unrelated to HSP70. Nucleic Acids Res. 1990 Jun 11;18(11):3409–3409. doi: 10.1093/nar/18.11.3409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boucher S. E., Gillin F. D. Excystation of in vitro-derived Giardia lamblia cysts. Infect Immun. 1990 Nov;58(11):3516–3522. doi: 10.1128/iai.58.11.3516-3522.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cheng M. Y., Hartl F. U., Horwich A. L. The mitochondrial chaperonin hsp60 is required for its own assembly. Nature. 1990 Nov 29;348(6300):455–458. doi: 10.1038/348455a0. [DOI] [PubMed] [Google Scholar]
  5. Das S., Reiner D. S., Zenian J., Hogan D. L., Koss M. A., Wang C. S., Gillin F. D. Killing of Giardia lamblia trophozoites by human intestinal fluid in vitro. J Infect Dis. 1988 Jun;157(6):1257–1260. doi: 10.1093/infdis/157.6.1257. [DOI] [PubMed] [Google Scholar]
  6. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  7. Erlandsen S. L., Bemrick W. J., Pawley J. High-resolution electron microscopic evidence for the filamentous structure of the cyst wall in Giardia muris and Giardia duodenalis. J Parasitol. 1989 Oct;75(5):787–797. [PubMed] [Google Scholar]
  8. Faubert G., Reiner D. S., Gillin F. D. Giardia lamblia: regulation of secretory vesicle formation and loss of ability to reattach during encystation in vitro. Exp Parasitol. 1991 May;72(4):345–354. doi: 10.1016/0014-4894(91)90080-g. [DOI] [PubMed] [Google Scholar]
  9. Gao B. C., Biosca J., Craig E. A., Greene L. E., Eisenberg E. Uncoating of coated vesicles by yeast hsp70 proteins. J Biol Chem. 1991 Oct 15;266(29):19565–19571. [PubMed] [Google Scholar]
  10. Gillin F. D., Boucher S. E., Rossi S. S., Reiner D. S. Giardia lamblia: the roles of bile, lactic acid, and pH in the completion of the life cycle in vitro. Exp Parasitol. 1989 Aug;69(2):164–174. doi: 10.1016/0014-4894(89)90185-9. [DOI] [PubMed] [Google Scholar]
  11. Gillin F. D., Hagblom P., Harwood J., Aley S. B., Reiner D. S., McCaffery M., So M., Guiney D. G. Isolation and expression of the gene for a major surface protein of Giardia lamblia. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4463–4467. doi: 10.1073/pnas.87.12.4463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gillin F. D., Reiner D. S., Boucher S. E. Small-intestinal factors promote encystation of Giardia lamblia in vitro. Infect Immun. 1988 Mar;56(3):705–707. doi: 10.1128/iai.56.3.705-707.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gillin F. D., Reiner D. S., Gault M. J., Douglas H., Das S., Wunderlich A., Sauch J. F. Encystation and expression of cyst antigens by Giardia lamblia in vitro. Science. 1987 Feb 27;235(4792):1040–1043. doi: 10.1126/science.3547646. [DOI] [PubMed] [Google Scholar]
  14. Keister D. B. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg. 1983;77(4):487–488. doi: 10.1016/0035-9203(83)90120-7. [DOI] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lindley T. A., Chakraborty P. R., Edlind T. D. Heat shock and stress response in Giardia lamblia. Mol Biochem Parasitol. 1988 Mar;28(2):135–143. doi: 10.1016/0166-6851(88)90061-8. [DOI] [PubMed] [Google Scholar]
  17. Nash T. E., Aggarwal A. Cytotoxicity of monoclonal antibodies to a subset of Giardia isolates. J Immunol. 1986 Apr 1;136(7):2628–2632. [PubMed] [Google Scholar]
  18. Plikaytis B. B., Carlone G. M., Pau C. P., Wilkinson H. W. Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes. J Clin Microbiol. 1987 Nov;25(11):2080–2084. doi: 10.1128/jcm.25.11.2080-2084.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. RENDTORFF R. C. The experimental transmission of human intestinal protozoan parasites. II. Giardia lamblia cysts given in capsules. Am J Hyg. 1954 Mar;59(2):209–220. doi: 10.1093/oxfordjournals.aje.a119634. [DOI] [PubMed] [Google Scholar]
  20. Reiner D. S., Douglas H., Gillin F. D. Identification and localization of cyst-specific antigens of Giardia lamblia. Infect Immun. 1989 Mar;57(3):963–968. doi: 10.1128/iai.57.3.963-968.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reiner D. S., McCaffery M., Gillin F. D. Sorting of cyst wall proteins to a regulated secretory pathway during differentiation of the primitive eukaryote, Giardia lamblia. Eur J Cell Biol. 1990 Oct;53(1):142–153. [PubMed] [Google Scholar]
  22. Schlesinger M. J. Heat shock proteins. J Biol Chem. 1990 Jul 25;265(21):12111–12114. [PubMed] [Google Scholar]
  23. Shinnick T. M., Sweetser D., Thole J., van Embden J., Young R. A. The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG. Infect Immun. 1987 Aug;55(8):1932–1935. doi: 10.1128/iai.55.8.1932-1935.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shinnick T. M., Vodkin M. H., Williams J. C. The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein. Infect Immun. 1988 Feb;56(2):446–451. doi: 10.1128/iai.56.2.446-451.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Taglicht D., Padan E., Oppenheim A. B., Schuldiner S. An alkaline shift induces the heat shock response in Escherichia coli. J Bacteriol. 1987 Feb;169(2):885–887. doi: 10.1128/jb.169.2.885-887.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Terlesky K. C., Tabita F. R. Purification and characterization of the chaperonin 10 and chaperonin 60 proteins from Rhodobacter sphaeroides. Biochemistry. 1991 Aug 20;30(33):8181–8186. doi: 10.1021/bi00247a013. [DOI] [PubMed] [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Van der Ploeg L. H., Giannini S. H., Cantor C. R. Heat shock genes: regulatory role for differentiation in parasitic protozoa. Science. 1985 Jun 21;228(4706):1443–1446. doi: 10.1126/science.4012301. [DOI] [PubMed] [Google Scholar]
  29. Zeilstra-Ryalls J., Fayet O., Georgopoulos C. The universally conserved GroE (Hsp60) chaperonins. Annu Rev Microbiol. 1991;45:301–325. doi: 10.1146/annurev.mi.45.100191.001505. [DOI] [PubMed] [Google Scholar]

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