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. 1991 Mar;59(3):852–856. doi: 10.1128/iai.59.3.852-856.1991

Mouse hepatitis virus strain UAB infection enhances resistance to Salmonella typhimurium in mice by inducing suppression of bacterial growth.

M T Fallon 1, W H Benjamin Jr 1, T R Schoeb 1, D E Briles 1
PMCID: PMC258337  PMID: 1847697

Abstract

We have previously shown that intranasal infection of mice with mouse hepatitis virus (MHV) strain UAB (MHV-UAB) increases their resistance to Salmonella typhimurium injected intravenously 6 days later. To study how salmonella resistance was induced, BALB/cAnNCr mice were infected with salmonella strains carrying specific genetic alterations. One set of studies compared the effect of MHV infection on subsequent salmonella infections with AroA- (avirulent) and Aro+ (virulent) salmonellae. Unlike its effect on Aro+ salmonellae, MHV failed to reduce the number of AroA- salmonellae recovered from mice. Because AroA- S. typhimurium shows almost no growth in vivo, this failure indicated that the effect of MHV on salmonella resistance required growth of the infecting salmonellae. In other studies, the effect of MHV infection on both growth and killing were monitored simultaneously in mice with growing salmonellae carrying a single copy of the temperature-sensitive pHSG422 plasmid, which is unable to replicate in vivo. MHV infection reduced salmonella growth but caused no increase in salmonella killing. MHV infection of mice given wild-type salmonellae also resulted in no increase in salmonella killing 4 h after salmonella challenge. These studies demonstrate that MHV-UAB infection increases host resistance to salmonellae by enhancing suppression of bacterial growth instead of by increasing the amount of salmonella killing.

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Selected References

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  1. Benjamin W. H., Jr, Hall P., Roberts S. J., Briles D. E. The primary effect of the Ity locus is on the rate of growth of Salmonella typhimurium that are relatively protected from killing. J Immunol. 1990 Apr 15;144(8):3143–3151. [PubMed] [Google Scholar]
  2. Benjamin W. H., Jr, Posey B. S., Briles D. E. Effects of in vitro growth phase on the pathogenesis of Salmonella typhimurium in mice. J Gen Microbiol. 1986 May;132(5):1283–1295. doi: 10.1099/00221287-132-5-1283. [DOI] [PubMed] [Google Scholar]
  3. Benjamin W. H., Jr, Turnbough C. L., Jr, Goguen J. D., Posey B. S., Briles D. E. Genetic mapping of novel virulence determinants of Salmonella typhimurium to the region between trpD and supD. Microb Pathog. 1986 Apr;1(2):115–124. doi: 10.1016/0882-4010(86)90014-8. [DOI] [PubMed] [Google Scholar]
  4. Benjamin W. H., Jr, Turnbough C. L., Jr, Posey B. S., Briles D. E. The ability of Salmonella typhimurium to produce the siderophore enterobactin is not a virulence factor in mouse typhoid. Infect Immun. 1985 Nov;50(2):392–397. doi: 10.1128/iai.50.2.392-397.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bukholm G., Berdal B. P., Haug C., Degré M. Mouse fibroblast interferon modifies Salmonella typhimurium infection in infant mice. Infect Immun. 1984 Jul;45(1):62–66. doi: 10.1128/iai.45.1.62-66.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bukholm G., Degré M. Effect of human leukocyte interferon on invasiveness of Salmonella species in HEp-2 cell cultures. Infect Immun. 1983 Dec;42(3):1198–1202. doi: 10.1128/iai.42.3.1198-1202.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casebolt D. B., Spalding D. M., Schoeb T. R., Lindsey J. R. Suppression of immune response induction in Peyer's patch lymphoid cells from mice infected with mouse hepatitis virus. Cell Immunol. 1987 Oct 1;109(1):97–103. doi: 10.1016/0008-8749(87)90295-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Collins F. M., Mackaness G. B., Blanden R. V. Infection-immunity in experimental salmonellosis. J Exp Med. 1966 Oct 1;124(4):601–619. doi: 10.1084/jem.124.4.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Drath D. B., Karnovsky M. L. Superoxide production by phagocytic leukocytes. J Exp Med. 1975 Jan 1;141(1):257–262. doi: 10.1084/jem.141.1.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fallon M. T., Schoeb T. R., Benjamin W. H., Jr, Lindsey J. R., Briles D. E. Modulation of resistance to Salmonella typhimurium infection in mice by mouse hepatitis virus (MHV). Microb Pathog. 1989 Feb;6(2):81–91. doi: 10.1016/0882-4010(89)90011-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fels A. O., Cohn Z. A. The alveolar macrophage. J Appl Physiol (1985) 1986 Feb;60(2):353–369. doi: 10.1152/jappl.1986.60.2.353. [DOI] [PubMed] [Google Scholar]
  12. Hoiseth S. K., Stocker B. A. Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines. Nature. 1981 May 21;291(5812):238–239. doi: 10.1038/291238a0. [DOI] [PubMed] [Google Scholar]
  13. JENKIN C. R., ROWLEY D. The role of opsonins in the clearance of living and inert particles by cells of the reticuloendothelial system. J Exp Med. 1961 Sep 1;114:363–374. doi: 10.1084/jem.114.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mackaness G. B., Blanden R. V., Collins F. M. Host-parasite relations in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):573–583. doi: 10.1084/jem.124.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mills E. L. Viral infections predisposing to bacterial infections. Annu Rev Med. 1984;35:469–479. doi: 10.1146/annurev.me.35.020184.002345. [DOI] [PubMed] [Google Scholar]
  16. Spitznagel J. K. Nonoxidative antimicrobial reactions of leukocytes. Contemp Top Immunobiol. 1984;14:283–343. doi: 10.1007/978-1-4757-4862-8_10. [DOI] [PubMed] [Google Scholar]

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