Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1991 Mar;59(3):1112–1116. doi: 10.1128/iai.59.3.1112-1116.1991

Effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of osteoblastic clone MC3T3-e1 cells.

Y Horiguchi 1, T Nakai 1, K Kume 1
PMCID: PMC258375  PMID: 1997414

Abstract

The effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of a clonal osteoblastic cell line, MC3T3-E1, were investigated. The toxin induced a morphological change in the cells from a spindle shape to a spherical form with many blebs. The toxin-treated cells were viable and grew to form confluent cell layers composed of irregularly shaped cells and multinuclear cells. The toxin inhibited elevation of alkaline phosphatase activity in the cells in a dose-dependent manner at concentrations from 10 pg to 10 ng/ml. The accumulation of type I collagen in the cells was also reduced by the toxin. Since high alkaline phosphatase activity and accumulation of collagen are closely linked to differentiation of the cells into osteoblasts, it is considered likely that B. bronchiseptica dermonecrotic toxin impairs the ability of the cells to differentiate.

Full text

PDF

Images in this article

1114Image
on p.1114

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Endoh M., Nagai M., Nakase Y. Contractile action of heat-labile toxin of Bordetella parapertussis on aortic smooth muscles of pigs. Microbiol Immunol. 1988;32(8):755–767. doi: 10.1111/j.1348-0421.1988.tb01437.x. [DOI] [PubMed] [Google Scholar]
  3. Fetter A. W., Capen C. C. Ultrastructural evaluation of bone cells in pigs with experimental turbinate osteoporosis (atrophic rhinitis). Lab Invest. 1971 May;24(5):392–403. [PubMed] [Google Scholar]
  4. Fetter A. W., Switzer W. P., Capen C. C. Electron microscopic evaluation of bone cells in pigs with experimentally induced Bordetella rhinitis (turbinate osteoporosis). Am J Vet Res. 1975 Jan;36(1):15–22. [PubMed] [Google Scholar]
  5. Hakeda Y., Hotta T., Kurihara N., Ikeda E., Maeda N., Yagyu Y., Kumegawa M. Prostaglandin E1 and F2 alpha stimulate differentiation and proliferation, respectively, of clonal osteoblastic MC3T3-E1 cells by different second messengers in vitro. Endocrinology. 1987 Dec;121(6):1966–1974. doi: 10.1210/endo-121-6-1966. [DOI] [PubMed] [Google Scholar]
  6. Hakeda Y., Ikeda E., Kurihara N., Nakatani Y., Maeda N., Kumegawa M. Induction of osteoblastic cell differentiation by forskolin. Stimulation of cyclic AMP production and alkaline phosphatase activity. Biochim Biophys Acta. 1985 Jan 28;838(1):49–53. doi: 10.1016/0304-4165(85)90248-x. [DOI] [PubMed] [Google Scholar]
  7. Hakeda Y., Nakatani Y., Hiramatsu M., Kurihara N., Tsunoi M., Ikeda E., Kumegawa M. Inductive effects of prostaglandins on alkaline phosphatase in osteoblastic cells, clone MC3T3-E1. J Biochem. 1985 Jan;97(1):97–104. doi: 10.1093/oxfordjournals.jbchem.a135072. [DOI] [PubMed] [Google Scholar]
  8. Hakeda Y., Nakatani Y., Yoshino T., Kurihara N., Fujita K., Maeda N., Kumegawa M. Effect of forskolin on collagen production in clonal osteoblastic MC3T3-E1 cells. J Biochem. 1987 Jun;101(6):1463–1469. doi: 10.1093/oxfordjournals.jbchem.a122016. [DOI] [PubMed] [Google Scholar]
  9. Hanada M., Shimoda K., Tomita S., Nakase Y., Nishiyama Y. Production of lesions similar to naturally occurring swine atrophic rhinitis by cell-free sonicated extract of Bordetella bronchiseptica. Nihon Juigaku Zasshi. 1979 Feb;41(1):1–8. doi: 10.1292/jvms1939.41.1. [DOI] [PubMed] [Google Scholar]
  10. Hata R., Hori H., Nagai Y., Tanaka S., Kondo M., Hiramatsu M., Utsumi N., Kumegawa M. Selective inhibition of type I collagen synthesis in osteoblastic cells by epidermal growth factor. Endocrinology. 1984 Sep;115(3):867–876. doi: 10.1210/endo-115-3-867. [DOI] [PubMed] [Google Scholar]
  11. Hiramatsu M., Ikeda E., Kashimata M., Minami N., Kodama H., Sudo H., Kumegawa M. Effect of dibutyryl cyclic AMP on collagen synthesis in a clonal osteoblast-like cell line derived from newborn mouse calvaria. J Biochem. 1983 Nov;94(5):1353–1358. doi: 10.1093/oxfordjournals.jbchem.a134481. [DOI] [PubMed] [Google Scholar]
  12. Horiguchi Y., Nakai T., Kume K. Purification and characterization of Bordetella bronchiseptica dermonecrotic toxin. Microb Pathog. 1989 May;6(5):361–368. doi: 10.1016/0882-4010(89)90078-8. [DOI] [PubMed] [Google Scholar]
  13. Horiguchi Y., Nakai T., Kume K. Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin. FEMS Microbiol Lett. 1990 Jan 1;54(1-3):39–43. doi: 10.1016/0378-1097(90)90255-o. [DOI] [PubMed] [Google Scholar]
  14. Iida T., Okonogi T. Lienotoxicity of Bordetella pertussis in mice. J Med Microbiol. 1971 Feb;4(1):51–61. doi: 10.1099/00222615-4-1-51. [DOI] [PubMed] [Google Scholar]
  15. Kimman T. G., Löwik C. W., van de Wee-Pals L. J., Thesingh C. W., Defize P., Kamp E. M., Bijvoet O. L. Stimulation of bone resorption by inflamed nasal mucosa, dermonecrotic toxin-containing conditioned medium from Pasteurella multocida, and purified dermonecrotic toxin from P. multocida. Infect Immun. 1987 Sep;55(9):2110–2116. doi: 10.1128/iai.55.9.2110-2116.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROBERTS N. R., WU M. L., HIXON W. S., CRAWFORD E. J. The quantitative histochemistry of brain. II. Enzyme measurements. J Biol Chem. 1954 Mar;207(1):19–37. [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Oyamada T., Yoshikawa T., Yoshikawa H., Shimizu M., Nakai T., Kume K. Lesions induced in the nasal turbinates of neonatal pigs inoculated with Pasteurella multocia and/or Bordetella bronchiseptica. Nihon Juigaku Zasshi. 1986 Apr;48(2):377–387. doi: 10.1292/jvms1939.48.377. [DOI] [PubMed] [Google Scholar]
  19. Sharp G. W., Hynie S. Stimulation of intestinal adenyl cyclase by cholera toxin. Nature. 1971 Jan 22;229(5282):266–269. doi: 10.1038/229266a0. [DOI] [PubMed] [Google Scholar]
  20. Silveira D., Edington N., Smith I. M. Ultrastructural changes in the nasal turbinate bones of pigs in early infection with Bordetella bronchiseptica. Res Vet Sci. 1982 Jul;33(1):37–42. [PubMed] [Google Scholar]
  21. Sudo H., Kodama H. A., Amagai Y., Yamamoto S., Kasai S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 1983 Jan;96(1):191–198. doi: 10.1083/jcb.96.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES