Abstract
The gene encoding damselysin, an extracellular cytolysin produced by virulent Vibrio damsela strains, was cloned and expressed in Escherichia coli. DNA sequences homologous to that of the cloned gene were detected in hemolytic strains of V. damsela but not in other hemolytic Vibrio species.
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- BERNHEIMER A. W., SCHWARTZ L. L. Isolation and composition of staphylococcal alpha toxin. J Gen Microbiol. 1963 Mar;30:455–468. doi: 10.1099/00221287-30-3-455. [DOI] [PubMed] [Google Scholar]
- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chakraborty T., Kathariou S., Hacker J., Hof H., Huhle B., Wagner W., Kuhn M., Goebel W. Molecular analysis of bacterial cytolysins. Rev Infect Dis. 1987 Sep-Oct;9 (Suppl 5):S456–S466. doi: 10.1093/clinids/9.supplement_5.s456. [DOI] [PubMed] [Google Scholar]
- Clarridge J. E., Zighelboim-Daum S. Isolation and characterization of two hemolytic phenotypes of Vibrio damsela associated with a fatal wound infection. J Clin Microbiol. 1985 Mar;21(3):302–306. doi: 10.1128/jcm.21.3.302-306.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coffey J. A., Jr, Harris R. L., Rutledge M. L., Bradshaw M. W., Williams T. W., Jr Vibrio damsela: another potentially virulent marine vibrio. J Infect Dis. 1986 Apr;153(4):800–802. doi: 10.1093/infdis/153.4.800-a. [DOI] [PubMed] [Google Scholar]
- Honda T., Ni Y. X., Miwatani T. Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin. Infect Immun. 1988 Apr;56(4):961–965. doi: 10.1128/iai.56.4.961-965.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaper J. B., Campen R. K., Seidler R. J., Baldini M. M., Falkow S. Cloning of the thermostable direct or Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus. Infect Immun. 1984 Jul;45(1):290–292. doi: 10.1128/iai.45.1.290-292.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kothary M. H., Kreger A. S. Purification and characterization of an extracellular cytolysin produced by Vibrio damsela. Infect Immun. 1985 Jul;49(1):25–31. doi: 10.1128/iai.49.1.25-31.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kreger A. S., Bernheimer A. W., Etkin L. A., Daniel L. W. Phospholipase D activity of Vibrio damsela cytolysin and its interaction with sheep erythrocytes. Infect Immun. 1987 Dec;55(12):3209–3212. doi: 10.1128/iai.55.12.3209-3212.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kreger A. S. Cytolytic activity and virulence of Vibrio damsela. Infect Immun. 1984 May;44(2):326–331. doi: 10.1128/iai.44.2.326-331.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kreger A. S., Kothary M. H., Gray L. D. Cytolytic toxins of Vibrio vulnificus and Vibrio damsela. Methods Enzymol. 1988;165:176–189. doi: 10.1016/s0076-6879(88)65028-2. [DOI] [PubMed] [Google Scholar]
- Lockwood D. E., Kreger A. S., Richardson S. H. Detection of toxins produced by vibrio fluvialis. Infect Immun. 1982 Feb;35(2):702–708. doi: 10.1128/iai.35.2.702-708.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Love M., Teebken-Fisher D., Hose J. E., Farmer J. J., 3rd, Hickman F. W., Fanning G. R. Vibrio damsela, a Marine Bacterium, Causes Skin Ulcers on the Damselfish Chromis punctipinnis. Science. 1981 Dec 4;214(4525):1139–1140. doi: 10.1126/science.214.4525.1139. [DOI] [PubMed] [Google Scholar]
- Manoil C., Beckwith J. TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129–8133. doi: 10.1073/pnas.82.23.8129. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller V. L., Mekalanos J. J. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol. 1988 Jun;170(6):2575–2583. doi: 10.1128/jb.170.6.2575-2583.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miyake M., Honda T., Miwatani T. Purification and characterization of Vibrio metschnikovii cytolysin. Infect Immun. 1988 Apr;56(4):954–960. doi: 10.1128/iai.56.4.954-960.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris J. G., Jr, Miller H. G., Wilson R., Tacket C. O., Hollis D. G., Hickman F. W., Weaver R. E., Blake P. A. Illness caused by Vibrio damsela and Vibrio hollisae. Lancet. 1982 Jun 5;1(8284):1294–1297. doi: 10.1016/s0140-6736(82)92853-7. [DOI] [PubMed] [Google Scholar]
- Okada K., Miake S., Moriya T., Mitsuyama M., Amako K. Variability of haemolysin(s) produced by Vibrio vulnificus. J Gen Microbiol. 1987 Oct;133(10):2853–2857. doi: 10.1099/00221287-133-10-2853. [DOI] [PubMed] [Google Scholar]
- Peterson K. M., Mekalanos J. J. Characterization of the Vibrio cholerae ToxR regulon: identification of novel genes involved in intestinal colonization. Infect Immun. 1988 Nov;56(11):2822–2829. doi: 10.1128/iai.56.11.2822-2829.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richardson K., Michalski J., Kaper J. B. Hemolysin production and cloning of two hemolysin determinants from classical Vibrio cholerae. Infect Immun. 1986 Nov;54(2):415–420. doi: 10.1128/iai.54.2.415-420.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Wright A. C., Morris J. G., Jr, Maneval D. R., Jr, Richardson K., Kaper J. B. Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus. Infect Immun. 1985 Dec;50(3):922–924. doi: 10.1128/iai.50.3.922-924.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto K., Al-Omani M., Honda T., Takeda Y., Miwatani T. Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin. Infect Immun. 1984 Jul;45(1):192–196. doi: 10.1128/iai.45.1.192-196.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoh M., Honda T., Miwatani T. Purification and partial characterization of a Vibrio hollisae hemolysin that relates to the thermostable direct hemolysin of Vibrio parahaemolyticus. Can J Microbiol. 1986 Aug;32(8):632–636. doi: 10.1139/m86-118. [DOI] [PubMed] [Google Scholar]