Figure 1.
A, LβT2 cells were transfected with 450 ng/well −1028/+7 hFSHB-luc or empty pA3-luc reporters and then treated in serum-free medium with 10−7 m GnRH1 for the indicated times (in hours). The data reflect the mean of triplicate (±sd) samples and are presented relative to the luciferase activity measured in control cells not exposed to ligand. Data were analyzed by one-way ANOVA (Tukey post hoc test). B, Cells were transfected as in A with the indicated human FSHB promoter-reporters in pA3-luc or with the empty vector. Cells were then treated for 6 h with the indicated concentrations of GnRH1. All treatments were performed in duplicate. Significant differences between treatments are indicated with symbols.