CELL BIOLOGY. For the article “miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely,” by Beiyan Zhou, Stephanie Wang, Christine Mayr, David P. Bartel, and Harvey F. Lodish, which appeared in issue 17, April 24, 2007, of Proc Natl Acad Sci USA (104:7080–7085; first published April 16, 2007; 10.1073/pnas.0702409104), the authors note that due to a printer's error, in Fig. 2 A and B, the x-axis labels in the Middle and Bottom panels appeared incorrectly. The corrected figure and its legend appear below.
Fig. 2.
Effects on short-term and long-term repopulation caused by overexpression of miR-150 and miR-195. (A and B) Lin− fetal liver cells from day 13.5–14.5 C57BL/6 mice (CD45.2) were prepared as described in Materials and Methods, spin-infected with miR-150 (Top), miR-195 (Middle), or empty MDH retroviral supernatant (Bottom), and transplanted into irradiated CD45.1 recipients. To evaluate the effects of short-term and long-term repopulation, peripheral blood samples were collected at 4 weeks (A) or 16 weeks (B) after transplantation. Donor-derived (CD45.2) cell lineages were examined by using antibodies against specific cell lineage surface markers followed by FACS analysis: B cells (CD19+), T cells (CD4+ or CD8+), and myeloid cells (CD11b+ or Gr-1+). The percentage of each cell lineage in either the GFP− or GFP+ population from each mouse was defined as that specific cell lineage proportion in the peripheral blood. The mean value for each group was the average of all cell proportions from 20 mice in two independent transplantations. (C) The long-term repopulation effects of the transplantation in the spleen (Left) and lymph nodes (Right) of miR-150 mice were examined as described. Statistics from each cell lineage were calculated by the Mann–Whitney test. Significance was defined as follows: *, P < 0.05; **, P < 0.001.