CELL BIOLOGY. For the article “Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium,” by Miho K. Furue, Jie Na, Jamie P. Jackson, Tetsuji Okamoto, Mark Jones, Duncan Baker, Ryu-Ichiro Hata, Harry D. Moore, J. Denry Sato, and Peter W. Andrews, which appeared in issue 36, September 9, 2008, of Proc Natl Acad Sci USA (105:13409–13414; first published August 25, 2008; 10.1073/pnas.0806136105), the authors note that in Fig. 7D, Q-PCR data were inadvertently duplicated. This error does not affect the conclusions of the article. The corrected figure and its legend appear below.
Fig. 7.
Long-term culture of HESCs in the defined medium. HUES-1 and Shef1 cells were serially cultured on type I collagen in hESF9. The cells were split at 1:3 every week. (A) Phase contrast photomicrograph of HUES-1 at passage 21 and Shef1 cells at passage 14. (Scale bar, 200 μm.) (B) Growth of HUES-1 (passage 10) and Shef1 (passage 5) cells in the defined medium. (C) Immunohistochemical staining of HUES-1 (passage 24) and Shef1 (passage 14) for SSEA-1, SSEA-3, and OCT3/4. (D) Q-PCR analysis of gene expression in HUES-1 and Shef1 on feeder in KSR-based medium (feeder) and HUES-1 (passage 24) and Shef1 (passage 14) on collagen in hESF9 medium (ESF) during in vitro differentiation (EB). The name of the gene of differentiation is noted in each bar graph. Expression levels were normalized against GAPDH. The relative level of each gene in undifferentiated cells was defined as “1.”