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. 1990 Feb;58(2):464–470. doi: 10.1128/iai.58.2.464-470.1990

Regulation of expression of Streptococcus mutans genes important to virulence.

M C Hudson 1, R Curtiss 3rd 1
PMCID: PMC258480  PMID: 2298487

Abstract

Studies were initiated to investigate the regulation of Streptococcus mutans genes which are believed to be important to virulence. Operon fusions were constructed between S. mutans gene regulatory regions and a promoterless chloramphenicol acetyltransferase gene (cat) found on the plasmid pMH109. Specifically, fusions were generated between cat and the S. mutans genes encoding fructosyltransferase (ftf) and the glucosyltransferase B/C (gtfB/C) operon. Constructs were confirmed by restriction enzyme analysis, and the fusions were subcloned into the integration vehicle pVA891. Following generation of multimeric DNA, recombinant plasmids were introduced into the s. mutans genome by Campbell-type insertion, resulting in single-copy operon fusions. Chloramphenicol acetyltransferase specific activities were used to monitor the expression of the S. mutans gtfB/C operon and ftf determinants. The expression of these genes is increased by the presence of sucrose and is followed by a rapid decline in expression over time. Additionally, expression of the gtfB/C operon is increased in S. mutans cells bound to artificial tooth pellicles.

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Selected References

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  1. Aoki H., Shiroza T., Hayakawa M., Sato S., Kuramitsu H. K. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis. Infect Immun. 1986 Sep;53(3):587–594. doi: 10.1128/iai.53.3.587-594.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Band L., Yansura D. G., Henner D. J. Construction of a vector for cloning promoters in Bacillus subtilis. Gene. 1983 Dec;26(2-3):313–315. doi: 10.1016/0378-1119(83)90204-4. [DOI] [PubMed] [Google Scholar]
  3. Barletta R. G., Curtiss R., 3rd Impairment of melibiose utilization in Streptococcus mutans serotype c gtfA mutants. Infect Immun. 1989 Mar;57(3):992–995. doi: 10.1128/iai.57.3.992-995.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barletta R. G., Michalek S. M., Curtiss R., 3rd Analysis of the virulence of Streptococcus mutans serotype c gtfA mutants in the rat model system. Infect Immun. 1988 Feb;56(2):322–330. doi: 10.1128/iai.56.2.322-330.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  6. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  7. Chassy B. M., Beall J. R., Bielawski R. M., Porter E. V., Donkersloot J. A. Occurrence and distribution of sucrose-metabolizing enzymes in oral streptococci. Infect Immun. 1976 Aug;14(2):408–415. doi: 10.1128/iai.14.2.408-415.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clark W. B., Bammann L. L., Gibbons R. J. Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun. 1978 Mar;19(3):846–853. doi: 10.1128/iai.19.3.846-853.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  10. Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hansen J. B., Abiko Y., Curtiss R., 3rd Characterization of the Streptococcus mutans plasmid pva318 cloned into Escherichia coli. Infect Immun. 1981 Mar;31(3):1034–1043. doi: 10.1128/iai.31.3.1034-1043.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hudson M. C., Stewart G. C. Differential utilization of Staphylococcus aureus promoter sequences by Escherichia coli and Bacillus subtilis. Gene. 1986;48(1):93–100. doi: 10.1016/0378-1119(86)90355-0. [DOI] [PubMed] [Google Scholar]
  13. Macrina F. L., Evans R. P., Tobian J. A., Hartley D. L., Clewell D. B., Jones K. R. Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning. Gene. 1983 Nov;25(1):145–150. doi: 10.1016/0378-1119(83)90176-2. [DOI] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Murchison H. H., Barrett J. F., Cardineau G. A., Curtiss R., 3rd Transformation of Streptococcus mutans with chromosomal and shuttle plasmid (pYA629) DNAs. Infect Immun. 1986 Nov;54(2):273–282. doi: 10.1128/iai.54.2.273-282.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Russell R. R., Mukasa H., Shimamura A., Ferretti J. J. Streptococcus mutans gtfA gene specifies sucrose phosphorylase. Infect Immun. 1988 Oct;56(10):2763–2765. doi: 10.1128/iai.56.10.2763-2765.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sato S., Kuramitsu H. K. Isolation and characterization of a fructosyltransferase gene from Streptococcus mutans GS-5. Infect Immun. 1986 Apr;52(1):166–170. doi: 10.1128/iai.52.1.166-170.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shaw W. V. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 1975;43:737–755. doi: 10.1016/0076-6879(75)43141-x. [DOI] [PubMed] [Google Scholar]
  19. Shiroza T., Kuramitsu H. K. Sequence analysis of the Streptococcus mutans fructosyltransferase gene and flanking regions. J Bacteriol. 1988 Feb;170(2):810–816. doi: 10.1128/jb.170.2.810-816.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shiroza T., Ueda S., Kuramitsu H. K. Sequence analysis of the gtfB gene from Streptococcus mutans. J Bacteriol. 1987 Sep;169(9):4263–4270. doi: 10.1128/jb.169.9.4263-4270.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tanzer J. M. Studies on the fate of the glucosyl moiety of sucrose metabolized by Streptococcus mutans. J Dent Res. 1972 Mar-Apr;51(2):415–423. doi: 10.1177/00220345720510023001. [DOI] [PubMed] [Google Scholar]
  22. Terleckyj B., Willett N. P., Shockman G. D. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun. 1975 Apr;11(4):649–655. doi: 10.1128/iai.11.4.649-655.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ueda S., Shiroza T., Kuramitsu H. K. Sequence analysis of the gtfC gene from Streptococcus mutans GS-5. Gene. 1988 Sep 15;69(1):101–109. doi: 10.1016/0378-1119(88)90382-4. [DOI] [PubMed] [Google Scholar]
  24. Wenham D. G., Hennessey T. D., Cole J. A. Regulation of glucosyl- and fructosyltransferase synthesis by continuous cultures of Streptococcus mutans. J Gen Microbiol. 1979 Sep;114(1):117–124. doi: 10.1099/00221287-114-1-117. [DOI] [PubMed] [Google Scholar]

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