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. 1990 Mar;58(3):695–702. doi: 10.1128/iai.58.3.695-702.1990

Purification and characterization of the Dr hemagglutinins expressed by two uropathogenic Escherichia coli strains.

M L Kist 1, I E Salit 1, T Hofmann 1
PMCID: PMC258521  PMID: 1968432

Abstract

The fibrillar Dr hemagglutinins expressed by two uropathogenic Escherichia coli isolates were mechanically sheared from whole cells and subsequently purified by using anion-exchange high-pressure liquid chromatography. The isolated hemagglutinins were proteins with apparent subunit molecular masses of 14,500 daltons by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric points of 5.4 in denaturing isoelectric focusing gels. The two proteins were serologically related to each other but distinct from P fimbriae, as assessed by bacterial agglutination and immunoblotting. The amino acid compositions of the two hemagglutinins were highly similar both to each other and to other Dr hemagglutinins. N-terminal amino acid sequencing of the major hemagglutinin subunit proteins demonstrated homology with afimbrial E. coli adhesins.

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Selected References

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