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. 1990 Mar;58(3):838–840. doi: 10.1128/iai.58.3.838-840.1990

Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed-field gel electrophoresis.

J J Tudor 1, L Marri 1, P J Piggot 1, L Daneo-Moore 1
PMCID: PMC258543  PMID: 2307521

Abstract

Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were greater than 100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 +/- 60 kilobase pairs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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