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. 1990 Apr;58(4):930–934. doi: 10.1128/iai.58.4.930-934.1990

Assay for enterotoxigenic Escherichia coli heat-stable toxin b in rats and mice.

S C Whipp 1
PMCID: PMC258562  PMID: 2180824

Abstract

The Escherichia coli heat-stable enterotoxin STb is the most prevalent toxin associated with diarrheagenic isolates of porcine origin. This report demonstrates that when endogenous protease activity was blocked with soybean trypsin inhibitor, STb evoked a dose-dependent secretory response in infant mice and jejunal loops of rats. Infant mice were much less sensitive to STb than rats were. The response of rat jejunal loops to STb was linearly related to the log of the dose of STb through 10 twofold dilutions. The anterior 25% of the jejunum was less sensitive than was the remainder of the gut. An incubation period of 2.5 h provided a maximal response. The linear response to STb in rats could be used as a bioassay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  2. Gyles C. L. Limitations of the infant mouse test for Escherichia coli heat stable enterotoxin. Can J Comp Med. 1979 Oct;43(4):371–379. [PMC free article] [PubMed] [Google Scholar]
  3. Handl C., Rönnberg B., Nilsson B., Olsson E., Jonsson H., Flock J. I. Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II. J Clin Microbiol. 1988 Aug;26(8):1555–1560. doi: 10.1128/jcm.26.8.1555-1560.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kennedy D. J., Greenberg R. N., Dunn J. A., Abernathy R., Ryerse J. S., Guerrant R. L. Effects of Escherichia coli heat-stable enterotoxin STb on intestines of mice, rats, rabbits, and piglets. Infect Immun. 1984 Dec;46(3):639–643. doi: 10.1128/iai.46.3.639-643.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lee C. H., Moseley S. L., Moon H. W., Whipp S. C., Gyles C. L., So M. Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers. Infect Immun. 1983 Oct;42(1):264–268. doi: 10.1128/iai.42.1.264-268.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Monckton R. P., Hasse D. Detection of enterotoxigenic Escherichia coli in piggeries in Victoria by DNA hybridisation using K88, K99, LT, ST1 and ST2 probes. Vet Microbiol. 1988 Mar;16(3):273–281. doi: 10.1016/0378-1135(88)90031-4. [DOI] [PubMed] [Google Scholar]
  7. Moon H. W., Fung P. Y., Isaacson R. E., Booth G. D. Effects of age, ambient temperature, and heat-stable Escherichia coli enterotoxin on intestinal transit in infant mice. Infect Immun. 1979 Jul;25(1):127–132. doi: 10.1128/iai.25.1.127-132.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moon H. W., Schneider R. A., Moseley S. L. Comparative prevalence of four enterotoxin genes among Escherichia coli isolated from swine. Am J Vet Res. 1986 Feb;47(2):210–212. [PubMed] [Google Scholar]
  9. Olsson E., Söderlind O. Comparison of different assays for definition of heat-stable enterotoxigenicity of Escherichia coli porcine strains. J Clin Microbiol. 1980 Jan;11(1):6–15. doi: 10.1128/jcm.11.1.6-15.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rose R., Whipp S. C., Moon H. W. Effects of Escherichia coli heat-stable enterotoxin b on small intestinal villi in pigs, rabbits, and lambs. Vet Pathol. 1987 Jan;24(1):71–79. doi: 10.1177/030098588702400112. [DOI] [PubMed] [Google Scholar]
  11. Weikel C. S., Guerrant R. L. STb enterotoxin of Escherichia coli: cyclic nucleotide-independent secretion. Ciba Found Symp. 1985;112:94–115. doi: 10.1002/9780470720936.ch6. [DOI] [PubMed] [Google Scholar]
  12. Weikel C. S., Nellans H. N., Guerrant R. L. In vivo and in vitro effects of a novel enterotoxin, STb, produced by Escherichia coli. J Infect Dis. 1986 May;153(5):893–901. doi: 10.1093/infdis/153.5.893. [DOI] [PubMed] [Google Scholar]
  13. Whipp S. C., Kokue E., Morgan R. W., Rose R., Moon H. W. Functional significance of histologic alterations induced by Escherichia coli pig-specific, mouse-negative, heat-stable enterotoxin (STb). Vet Res Commun. 1987;11(1):41–55. doi: 10.1007/BF00361325. [DOI] [PubMed] [Google Scholar]
  14. Whipp S. C., Moon H. W., Lyon N. C. Heat-stable Escherichia coli enterotoxin production in vivo. Infect Immun. 1975 Aug;12(2):240–244. doi: 10.1128/iai.12.2.240-244.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Whipp S. C. Protease degradation of Escherichia coli heat-stable, mouse-negative, pig-positive enterotoxin. Infect Immun. 1987 Sep;55(9):2057–2060. doi: 10.1128/iai.55.9.2057-2060.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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