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. 1990 Apr;58(4):1026–1029. doi: 10.1128/iai.58.4.1026-1029.1990

Effect of environmental conditions on production of toxic shock syndrome toxin 1 by Staphylococcus aureus.

A C Wong 1, M S Bergdoll 1
PMCID: PMC258577  PMID: 2108084

Abstract

The kinetics of toxic shock syndrome toxin 1 (TSST-1) production by Staphylococcus aureus was studied in a fermentor in which aeration rate, atmospheric composition, pH, and temperature were controlled. The toxin was synthesized at a maximal rate during the exponential phase. High bacterial populations were not necessarily accompanied by high TSST-1 yields. Aerobiosis increased TSST-1 production, but excessive aeration had an adverse effect. Addition of CO2 enhanced TSST-1 yield by increasing toxin production rate and efficiency. Cultures with no pH control made more TSST-1 than those maintained at pH 5.5 to 7.5. Maximum TSST-1 yields were obtained when cultures were supplied with air (20 cm3/min) and CO2 (5 cm3/min) via a sintered glass sparger.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlson E. C. A CO2-enhanced hemolytic activity of Staphylococcus aureus associated with toxic shock syndrome: inhibition by agar. J Infect Dis. 1986 Jul;154(1):186–188. doi: 10.1093/infdis/154.1.186. [DOI] [PubMed] [Google Scholar]
  2. Carpenter D. F., Silverman G. J. Staphylococcal enterotoxin B and nuclease production under controlled dissolved oxygen conditions. Appl Microbiol. 1974 Oct;28(4):628–637. doi: 10.1128/am.28.4.628-637.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Engels W., Kamps M. A., van Boven C. P. Continuous culture studies on the production of staphylocoagulase by Staphylococcus aureus. Antonie Van Leeuwenhoek. 1980;46(6):533–550. doi: 10.1007/BF00394010. [DOI] [PubMed] [Google Scholar]
  4. Freed R. C., Evenson M. L., Reiser R. F., Bergdoll M. S. Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods. Appl Environ Microbiol. 1982 Dec;44(6):1349–1355. doi: 10.1128/aem.44.6.1349-1355.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUNT G. A., GOUREVITCH A., LEIN J. Preservation of cultures by drying on porcelain beads. J Bacteriol. 1958 Oct;76(4):453–454. doi: 10.1128/jb.76.4.453-454.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kass E. H., Kendrick M. I., Tsai Y. C., Parsonnet J. Interaction of magnesium ion, oxygen tension, and temperature in the production of toxic-shock-syndrome toxin-1 by Staphylococcus aureus. J Infect Dis. 1987 Apr;155(4):812–815. doi: 10.1093/infdis/155.4.812. [DOI] [PubMed] [Google Scholar]
  7. Kato E., Khan M., Kujovich L., Bergdoll M. S. Production of enterotoxin a. Appl Microbiol. 1966 Nov;14(6):966–972. doi: 10.1128/am.14.6.966-972.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Møller M., Thomsen A. C., Borch K., Dinesen K., Zdravkovic M. Rupture of fetal membranes and premature delivery associated with group B streptococci in urine of pregnant women. Lancet. 1984 Jul 14;2(8394):69–70. doi: 10.1016/s0140-6736(84)90242-3. [DOI] [PubMed] [Google Scholar]
  10. Reiser R. F., Robbins R. N., Khoe G. P., Bergdoll M. S. Purification and some physicochemical properties of toxic-shock toxin. Biochemistry. 1983 Aug 2;22(16):3907–3912. doi: 10.1021/bi00285a028. [DOI] [PubMed] [Google Scholar]
  11. Sarafian S. K., Morse S. A. Environmental factors affecting toxic shock syndrome toxin-1 (TSST-1) synthesis. J Med Microbiol. 1987 Aug;24(1):75–81. doi: 10.1099/00222615-24-1-75. [DOI] [PubMed] [Google Scholar]
  12. Schlievert P. M., Blomster D. A. Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors. J Infect Dis. 1983 Feb;147(2):236–242. doi: 10.1093/infdis/147.2.236. [DOI] [PubMed] [Google Scholar]
  13. Taylor D., Holland K. T. Effect of dilution rate and Mg2+ limitation on toxic shock syndrome toxin-1 production by Staphylococcus aureus grown in defined continuous culture. J Gen Microbiol. 1988 Mar;134(3):719–723. doi: 10.1099/00221287-134-3-719. [DOI] [PubMed] [Google Scholar]
  14. Todd J. K., Todd B. H., Franco-Buff A., Smith C. M., Lawellin D. W. Influence of focal growth conditions on the pathogenesis of toxic shock syndrome. J Infect Dis. 1987 Apr;155(4):673–681. doi: 10.1093/infdis/155.4.673. [DOI] [PubMed] [Google Scholar]
  15. Wagner G., Bohr L., Wagner P., Petersen L. N. Tampon-induced changes in vaginal oxygen and carbon dioxide tensions. Am J Obstet Gynecol. 1984 Jan 15;148(2):147–150. doi: 10.1016/s0002-9378(84)80165-9. [DOI] [PubMed] [Google Scholar]

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