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. 1990 Apr;58(4):1085–1092. doi: 10.1128/iai.58.4.1085-1092.1990

Phagocyte function in juvenile periodontitis.

H Repo 1, L Saxén 1, M Jäättelä 1, M Ristola 1, M Leirisalo-Repo 1
PMCID: PMC258586  PMID: 2318531

Abstract

We studied the chemotaxis of peripheral blood polymorphonuclear leukocytes (PMNs) and monocytes and the production of tumor necrosis factor alpha by monocytes of patients with juvenile periodontitis (JP). As a group, the patients' PMNs showed significantly increased chemotaxis determined by counting the number of migrating cells within a 3-microns-pore-size filter. Determined as distance of migration within the filter, as chemotactic increment based on checkerboard analysis, as leukotactic index calculated on the basis of distance of migration and cell count at different depths within a 3-microns-pore-size filter, as distance of migration under agarose, and as the number of PMNs migrating across a 5-microns-pore-size filter, the chemotactic migration rates of PMNs of patients were similar to those of controls. Evaluation of the data on an individual basis suggested that in terms of PMN chemotaxis some patients were hyperresponsive and some were hyporesponsive. Chemotaxis, spontaneous migration, and the rates of lipopolysaccharide-induced tumor necrosis factor alpha production by JP monocytes were similar to those of control cells. Our results give credence to the view that there are minor aberrations in the functions of JP phagocytes, but the extent to which these aberrations are relevant to accumulation of PMNs at sites of infection and inflammation in vivo and possibly contribute to the pathogenesis of JP remains unclear.

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Selected References

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  1. Agarwal S., Reynolds M. A., Duckett L. D., Suzuki J. B. Altered free cytosolic calcium changes and neutrophil chemotaxis in patients with juvenile periodontitis. J Periodontal Res. 1989 Mar;24(2):149–154. doi: 10.1111/j.1600-0765.1989.tb00870.x. [DOI] [PubMed] [Google Scholar]
  2. Altman L. C., Page R. C., Vandesteen G. E., Dixon L. I., Bradford C. Abnormalities of leukocyte chemotaxis in patients with various forms of periodontitis. J Periodontal Res. 1985 Nov;20(6):553–563. doi: 10.1111/j.1600-0765.1985.tb00839.x. [DOI] [PubMed] [Google Scholar]
  3. Anderson D. C., Schmalstieg F. C., Arnaout M. A., Kohl S., Tosi M. F., Dana N., Buffone G. J., Hughes B. J., Brinkley B. R., Dickey W. D. Abnormalities of polymorphonuclear leukocyte function associated with a heritable deficiency of high molecular weight surface glycoproteins (GP138): common relationship to diminished cell adherence. J Clin Invest. 1984 Aug;74(2):536–551. doi: 10.1172/JCI111451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Asman B., Engström P. E., Olsson T., Bergström K. Increased luminol enhanced chemiluminescence from peripheral granulocytes in juvenile periodontitis. Scand J Dent Res. 1984 Jun;92(3):218–223. doi: 10.1111/j.1600-0722.1984.tb00882.x. [DOI] [PubMed] [Google Scholar]
  5. BOYDEN S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962 Mar 1;115:453–466. doi: 10.1084/jem.115.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Baer P. N. The case for periodontosis as a clinical entity. J Periodontol. 1971 Aug;42(8):516–520. doi: 10.1902/jop.1971.42.8.516. [DOI] [PubMed] [Google Scholar]
  7. Bass D. A., Olbrantz P., Szejda P., Seeds M. C., McCall C. E. Subpopulations of neutrophils with increased oxidative product formation in blood of patients with infection. J Immunol. 1986 Feb 1;136(3):860–866. [PubMed] [Google Scholar]
  8. Bender J. G., McPhail L. C., Van Epps D. E. Exposure of human neutrophils to chemotactic factors potentiates activation of the respiratory burst enzyme. J Immunol. 1983 May;130(5):2316–2323. [PubMed] [Google Scholar]
  9. Bertolini D. R., Nedwin G. E., Bringman T. S., Smith D. D., Mundy G. R. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6;319(6053):516–518. doi: 10.1038/319516a0. [DOI] [PubMed] [Google Scholar]
  10. Cainciola L. J., Genco R. J., Patters M. R., McKenna J., van Oss C. J. Defective polymorphonuclear leukocyte function in a human periodontal disease. Nature. 1977 Feb 3;265(5593):445–447. doi: 10.1038/265445a0. [DOI] [PubMed] [Google Scholar]
  11. Clark R. A., Kimball H. R. Defective granulocyte chemotaxis in the Chediak-Higashi syndrome. J Clin Invest. 1971 Dec;50(12):2645–2652. doi: 10.1172/JCI106765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Clark R. A., Page R. C., Wilde G. Defective neutrophil chemotaxis in juvenile periodontitis. Infect Immun. 1977 Dec;18(3):694–700. doi: 10.1128/iai.18.3.694-700.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Donabedian H., Gallin J. I. Deactivation of human neutrophil chemotaxis by chemoattractants: effect on receptors for the chemotactic factor f-Met-Leu-Phe. J Immunol. 1981 Sep;127(3):839–844. [PubMed] [Google Scholar]
  14. Ellegaard B., Borregaard N., Ellegaard J. Neutrophil chemotaxis and phagocytosis in juvenile periodontitis. J Periodontal Res. 1984 May;19(3):261–268. doi: 10.1111/j.1600-0765.1984.tb00817.x. [DOI] [PubMed] [Google Scholar]
  15. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gallin J. I. Human neutrophil heterogeneity exists, but is it meaningful? Blood. 1984 May;63(5):977–983. [PubMed] [Google Scholar]
  17. Genco R. J., Van Dyke T. E., Levine M. J., Nelson R. D., Wilson M. E. 1985 Kreshover lecture. Molecular factors influencing neutrophil defects in periodontal disease. J Dent Res. 1986 Dec;65(12):1379–1391. doi: 10.1177/00220345860650120201. [DOI] [PubMed] [Google Scholar]
  18. Henry C. A., Thaweboon B., Sirisinha S. Neutrophil chemotaxis and periodontal health relationships of young adult people in Thailand. Arch Oral Biol. 1984;29(8):617–622. doi: 10.1016/0003-9969(84)90131-6. [DOI] [PubMed] [Google Scholar]
  19. Kinane D. F., Cullen C. F., Johnston F. A., Evans C. W. Neutrophil chemotactic behaviour in patients with early-onset forms of periodontitis (I). Leading front analysis in Boyden chambers. J Clin Periodontol. 1989 Apr;16(4):242–246. doi: 10.1111/j.1600-051x.1989.tb01648.x. [DOI] [PubMed] [Google Scholar]
  20. Kinane D. F., Cullen C. F., Johnston F. A., Evans C. W. Neutrophil chemotactic behaviour in patients with early-onset forms of periodontitis (II). Assessment using the under agarose technique. J Clin Periodontol. 1989 Apr;16(4):247–251. doi: 10.1111/j.1600-051x.1989.tb01649.x. [DOI] [PubMed] [Google Scholar]
  21. Larjava H., Saxén L., Kosunen T., Gahmberg C. G. Chemotaxis and surface glycoproteins of neutrophil granulocytes from patients with juvenile periodontitis. Arch Oral Biol. 1984;29(11):935–939. doi: 10.1016/0003-9969(84)90094-3. [DOI] [PubMed] [Google Scholar]
  22. Lavine W. S., Maderazo E. G., Stolman J., Ward P. A., Cogen R. B., Greenblatt I., Robertson P. B. Impaired neutrophil chemotaxis in patients with juvenile and rapidly progressing periodontitis. J Periodontal Res. 1979 Jan;14(1):10–19. doi: 10.1111/j.1600-0765.1979.tb00213.x. [DOI] [PubMed] [Google Scholar]
  23. Leirisalo M., Repo H., Tiilikainen A., Kosunen T. U., Laitinen O. Chemotaxis in yersinia arthritis. HLA-B27 positive neutrophils show high stimulated motility in vitro. Arthritis Rheum. 1980 Sep;23(9):1036–1044. doi: 10.1002/art.1780230911. [DOI] [PubMed] [Google Scholar]
  24. Link A. S., Jr, Bass D. A., McCall C. E. Altered neutrophil migration during bacterial infection associated with a serum modulator of cellular motility. J Infect Dis. 1979 Oct;140(4):517–526. doi: 10.1093/infdis/140.4.517. [DOI] [PubMed] [Google Scholar]
  25. Matsushima K., Morishita K., Yoshimura T., Lavu S., Kobayashi Y., Lew W., Appella E., Kung H. F., Leonard E. J., Oppenheim J. J. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. J Exp Med. 1988 Jun 1;167(6):1883–1893. doi: 10.1084/jem.167.6.1883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Noguchi K., Morita I., Ishikawa I., Murota S. Impaired polymorphonuclear leukocyte 15-lipoxygenase activity in juvenile and rapidly progressive periodontitis. Prostaglandins Leukot Essent Fatty Acids. 1988 Aug;33(2):137–141. doi: 10.1016/0952-3278(88)90153-6. [DOI] [PubMed] [Google Scholar]
  27. Page R. C., Clagett J. A., Engel L. D., Wilde G., Sims T. Effects of prostaglandin on the antigen- and mitogen-driven responses of peripheral blood lymphocytes from patients with adult and juvenile periodontitis. Clin Immunol Immunopathol. 1978 Sep;11(1):77–87. doi: 10.1016/0090-1229(78)90205-2. [DOI] [PubMed] [Google Scholar]
  28. Page R. C., Sims T. J., Geissler F., Altman L. C., Baab D. A. Abnormal leukocyte motility in patients with early-onset periodontitis. J Periodontal Res. 1984 Nov;19(6):591–594. doi: 10.1111/j.1600-0765.1984.tb01321.x. [DOI] [PubMed] [Google Scholar]
  29. Page R. C., Vandesteen G. E., Ebersole J. L., Williams B. L., Dixon I. L., Altman L. C. Clinical and laboratory studies of a family with a high prevalence of juvenile periodontitis. J Periodontol. 1985 Oct;56(10):602–610. doi: 10.1902/jop.1985.56.10.602. [DOI] [PubMed] [Google Scholar]
  30. Pedersen M. M. Chemotactic response of neutrophil polymorphonuclear leukocytes in juvenile periodontitis measured by the Leading Front method. Scand J Dent Res. 1988 Oct;96(5):421–427. doi: 10.1111/j.1600-0722.1988.tb01578.x. [DOI] [PubMed] [Google Scholar]
  31. Pedersen M. M. Comparison of two methods for measurement of chemotaxis of neutrophil polymorphonuclear leukocytes in vitro. Acta Pathol Microbiol Immunol Scand C. 1987 Oct;95(5):189–193. doi: 10.1111/j.1699-0463.1987.tb00029.x. [DOI] [PubMed] [Google Scholar]
  32. Repo H., Jokipii A. M., Leirisalo M., Kosunen T. U. Leucocyte motility in the newborn: determination of spontaneous movement is essential in the in vitro assessment of neutrophil chemotaxis. Clin Exp Immunol. 1980 Jun;40(3):620–626. [PMC free article] [PubMed] [Google Scholar]
  33. Repo H., Jättelä M., Leirisalo-Repo M., Hurme M. Production of tumour necrosis factor and interleukin 1 by monocytes of patients with previous Yersinia arthritis. Clin Exp Immunol. 1988 Jun;72(3):410–414. [PMC free article] [PubMed] [Google Scholar]
  34. Repo H., Leirisalo M., Kosunen T. U. Neutrophil chemotaxis under agarose: a statistical analysis and comparison of the chemotactic response of cells from different donors. J Immunol Methods. 1981;46(2):227–242. doi: 10.1016/0022-1759(81)90139-3. [DOI] [PubMed] [Google Scholar]
  35. Repo H., Leirisalo M., Tiilikainen A., Laitinen O. Chemotaxis in yersinia arthritis. In vitro stimulation of neutrophil migration by HLA--B27 positive and negative sera. Arthritis Rheum. 1982 Jun;25(6):655–661. doi: 10.1002/art.1780250608. [DOI] [PubMed] [Google Scholar]
  36. Repo H., Savilahti E., Leirisalo-Repo M. Aberrant phagocyte function in Shwachman syndrome. Clin Exp Immunol. 1987 Jul;69(1):204–212. [PMC free article] [PubMed] [Google Scholar]
  37. Rocklin R. E., Bendtzen K., Greineder D. Mediators of immunity: lymphokines and monokines. Adv Immunol. 1980;29:55–136. doi: 10.1016/s0065-2776(08)60043-7. [DOI] [PubMed] [Google Scholar]
  38. Smith S., Bick P. H., Miller G. A., Ranney R. R., Rice P. L., Lalor J. H., Tew J. G. Polyclonal B-cell activation: severe periodontal disease in young adults. Clin Immunol Immunopathol. 1980 Jul;16(3):354–366. doi: 10.1016/0090-1229(80)90141-5. [DOI] [PubMed] [Google Scholar]
  39. Suzuki J. B., Collison B. C., Falkler W. A., Jr, Nauman R. K. Immunologic profile of juvenile periodontitis. II. Neutrophil chemotaxis, phagocytosis and spore germination. J Periodontol. 1984 Aug;55(8):461–467. doi: 10.1902/jop.1984.55.8.461. [DOI] [PubMed] [Google Scholar]
  40. Suzuki J. B., Risom L., Falkler W. A., Jr, Collison C., Bowers G. Effect of periodontal therapy on spontaneous lymphocyte response and neutrophil chemotaxis in localized and generalized juvenile periodontitis patients. J Clin Periodontol. 1985 Feb;12(2):124–134. doi: 10.1111/j.1600-051x.1985.tb01371.x. [DOI] [PubMed] [Google Scholar]
  41. Valone F. H. Regulation of human leukocyte function by lipoxygenase products of arachidonic acid. Contemp Top Immunobiol. 1984;14:155–170. doi: 10.1007/978-1-4757-4862-8_5. [DOI] [PubMed] [Google Scholar]
  42. Van Dyke T. E., Horoszewicz H. U., Cianciola L. J., Genco R. J. Neutrophil chemotaxis dysfunction in human periodontitis. Infect Immun. 1980 Jan;27(1):124–132. doi: 10.1128/iai.27.1.124-132.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Van Dyke T. E., Horoszewicz H. U., Genco R. J. The polymorphonuclear leukocyte (PMNL) locomotor defect in juvenile periodontitis. Study of random migration, chemokinesis and chemotaxis. J Periodontol. 1982 Nov;53(11):682–687. doi: 10.1902/jop.1982.53.11.682. [DOI] [PubMed] [Google Scholar]
  44. Van Dyke T. E., Levine M. J., Genco R. J. Neutrophil function and oral disease. J Oral Pathol. 1985 Feb;14(2):95–120. doi: 10.1111/j.1600-0714.1985.tb00474.x. [DOI] [PubMed] [Google Scholar]
  45. Van Dyke T. E., Levine M. J., Tabak L. A., Genco R. J. Juvenile periodontitis as a model for neutrophil function: reduced binding of the complement chemotactic fragment, C5a. J Dent Res. 1983 Aug;62(8):870–872. doi: 10.1177/00220345830620080301. [DOI] [PubMed] [Google Scholar]
  46. Van Dyke T. E., Levine M. J., Tabak L. A., Genco R. J. Reduced chemotactic peptide binding in juvenile periodontitis: a model for neutrophil function. Biochem Biophys Res Commun. 1981 Jun 16;100(3):1278–1284. doi: 10.1016/0006-291x(81)91962-8. [DOI] [PubMed] [Google Scholar]
  47. Van Dyke T. E., Schweinebraten M., Cianciola L. J., Offenbacher S., Genco R. J. Neutrophil chemotaxis in families with localized juvenile periodontitis. J Periodontal Res. 1985 Sep;20(5):503–514. doi: 10.1111/j.1600-0765.1985.tb00834.x. [DOI] [PubMed] [Google Scholar]
  48. Van Dyke T. E., Wilson-Burrows C., Offenbacher S., Henson P. Association of an abnormality of neutrophil chemotaxis in human periodontal disease with a cell surface protein. Infect Immun. 1987 Sep;55(9):2262–2267. doi: 10.1128/iai.55.9.2262-2267.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Van Dyke T. E., Zinney W., Winkel K., Taufiq A., Offenbacher S., Arnold R. R. Neutrophil function in localized juvenile periodontitis. Phagocytosis, superoxide production and specific granule release. J Periodontol. 1986 Nov;57(11):703–708. doi: 10.1902/jop.1986.57.11.703. [DOI] [PubMed] [Google Scholar]
  50. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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