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. 2008 Dec;10(12):1362–1372. doi: 10.1593/neo.08784

Figure 4.

Figure 4

The PTT assay shows truncated Nf1 peptides in ovarian carcinoma cell lines and primary tumors. (A) PTT was performed using five overlapping cDNA segments encompassing the entire NF1 coding region. Representative examples of truncated peptides in segments II and III from the indicated cell lines are shown. The TOV21G cell line expresses normal levels of full-length NF1, and it was used to determine the size of the expected (normal) PTT products in each segment. (B) Representative examples of truncated peptides in Segments III, IV and V from the indicated OSC samples are shown. The TOV21G cell line expresses normal levels of NF1, and it was used to determine the size of the expected (normal) PTT products in each segment.