Table 1.

Sequence of Primers Used for PCR Assays.

Forward	Reverse
5′-GAG GAG AAG CGC AGT CAA TC-3′	5′-GAA GAG GAA GAA CAC GCA CA-3′
5′-CCA CCT CGA GAT TTC ACG TA-3′	5′-TTT TCC TAC CTA TCT GCA TCA CC-3′
5′-GCC AGT GAT CCC TTG TCC T-3′	5′-GGG CAA GGT GAG AAG ACA GA-3′
5′-CAT CTG GCT CCT CCA TGA AT-3′	5′-GGT GGC ACC CAC TAC TTG-3′
5′-ATG AGT CCA ACA TGC AGA GA-3′	5′-GTA ACT GTA TCA GTG AAA AT-3′
5′-ATG AGT CCA ACA TGC AGA GA-3′	5′-GTT GGC AGG CAC AGC TGG TT-3′
5′-AAC CAG CTG TGC CTG CCA AC-3′	5′-GTA ACT GTA TCA GTG AAA AT-3′

The PCR conditions were optimized to amplify a specific band of expected size using DNA samples as templates. The NACE-enriched WNT5A 5′-upstream element (AB) was amplified totally or partially (subfragments A and B) before cloning.