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. 1990 Jun;58(6):1545–1551. doi: 10.1128/iai.58.6.1545-1551.1990

Pilins from the B serogroup of Bacteroides nodosus: characterization, expression, and cross-protection.

T C Elleman 1, D J Stewart 1, K G Finney 1, P A Hoyne 1, C W Ward 1
PMCID: PMC258671  PMID: 1971255

Abstract

Sequences of pilin genes from four strains of serogroup B of the ovine pathogen Bacteroides nodosus have been determined. These sequences permit comparisons of amino acid sequence between pilins from different subtypes (B1, B2, B3, B4) of the B serogroup and assessment of intraserogroup variation. Pili of B. nodosus strains 234 (B1) and 183 (B2) were produced by Pseudomonas aeruginosa harboring a plasmid-borne B. nodosus pilin gene, and these pili were used in sheep vaccination trials. Pili from strain 183 (B2) were found to be a senior antigen to pili from strains of other B subtypes, providing protection against footrot infection caused by strains of the other B subtypes. Pili of this strain are therefore the most suitable candidate for inclusion in a pilus-based vaccine. Pili of strain 234 from subtype B1, the reference strain of the B serogroup, provided poor protection against infection with other subtypes.

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Selected References

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