FIGURE 2. TRAMP mice are tolerant towards the tumor-associated SV40_IV epitope.

A-B, TRAMP mice and as a control non-transgenic littermates received an intranasal flu-T infection. 10 days post infection, spleen cells were isolated and cultured in the presence of either SV40_404-411 or (as a control) the influenza A nucleoprotein derived PR_366-374 epitope (5×10^-4 μg/ml) for 14 days. Antigen specific T cells were measured by intracellular IFNγ staining after incubation with 100 ng/ml of the relevant peptide. Shown are dot-plots of 2 representative cultures (A) and cumulative data of all cultures (B). Numbers in upper right corner of dot-plots refer to percentage of IFNγ⁺ CD8⁺ cells of total CD8⁺ cells. Circles in graphs represent individual mice, bars indicate averages.