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. 1990 Jun;58(6):1812–1816. doi: 10.1128/iai.58.6.1812-1816.1990

Detection of antigens with affinity for host cell membrane polypeptides in culture supernatants of Trypanosoma cruzi.

C D Davis 1, R E Kuhn 1
PMCID: PMC258728  PMID: 2187810

Abstract

Parasite antigens which bind to host cell molecules of approximately 32 and 34 kilodaltons (kDa) were identified in supernatant fluids obtained from axenic cultures of Trypanosoma cruzi. These parasite components were first detected in culture supernatants obtained after 2 weeks in culture. Immunoblot analysis of culture supernatants exhibiting binding activity revealed the presence of several parasite antigens ranging in molecular mass from approximately 26 to 290 kDa. Gel filtration (Sephacryl S-300) analysis of culture supernatants revealed four major peaks, but only the highest-molecular-mass peak (containing several parasite antigens ranging from 27 to 250 kDa) possessed binding activity for the host cell molecules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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