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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Prostate. 2008 Sep 15;68(13):1443–1449. doi: 10.1002/pros.20810

Figure 4.

Figure 4

Expression of IL-6, IL-8 and VEGF is up-regulated via NF-κB-dependent mechanism in zinc-deficient PC-3 cells. Cells were cultured in the presence of TPEN (6 μM) for 18 hours. Expression of cytokines was examined in the cell culture supernatants using ELISA kits. To evaluate the contribution of NF-κB signaling for TPEN-induced IL-6, IL-8 and VEGF expression, PC-3 cells were pre-incubated with IκBαM adenovirus (1:500) for 24 hours prior to TPEN stimulation. Statistical analysis was performed by one-way ANOVA. * Statistically significant (p < 0.05) compared with cells cultured in medium alone.