Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1990 Jul;58(7):2283–2288. doi: 10.1128/iai.58.7.2283-2288.1990

Immunity to experimental fowl typhoid in chickens induced by a virulence plasmid-cured derivative of Salmonella gallinarum.

P A Barrow 1
PMCID: PMC258809  PMID: 2194968

Abstract

Chickens were immunized by two intramuscular inoculations at 1 and 14 days of age with virulence plasmid-cured derivatives of Salmonella gallinarum and were challenged 14 days later by oral inoculation of ca. 50 50% lethal doses (LD50) of fully virulent S. gallinarum 9. Mortality in the nonimmunized and immunized groups were 36 and 3%, respectively. This difference was highly significant (P less than 0.01). A significant reduction in mortality was also produced following oral challenge with 5,000 LD50 doses. The LD50 values by intramuscular inoculation of the challenge organism into nonimmunized and immunized chickens were log10 (0.13 +/- 1.57) and (9.74 +/- 2.72), respectively. Immunization was effective whether chickens were immunized at 1 and 14 days of age or at 21 and 35 days of age. Serum agglutinins were present in immunized chickens. Immunization with plasmid-cured Salmonella pullorum gave less protection, and immunization with Escherichia coli K-12 possessing the virulence plasmid of S. gallinarum gave none. The plasmid-cured S. gallinarum was made both rough by virulent bacteriophage activity and nalidixic acid resistant (Nalr) to produce a strain designated 9VP-phi rNalr. It was compared with a Nalr mutant of the rough 9R vaccine strain designated 9 Nalr for virulence and immunogenicity. 9VP-phi rNalr was slightly less protective and less virulent than was the 9R vaccine strain.

Full text

PDF
2283

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baird G. D., Manning E. J., Jones P. W. Evidence for related virulence sequences in plasmids of Salmonella dublin and Salmonella typhimurium. J Gen Microbiol. 1985 Jul;131(7):1815–1823. doi: 10.1099/00221287-131-7-1815. [DOI] [PubMed] [Google Scholar]
  2. Barrow P. A., Lovell M. A. Functional homology of virulence plasmids in Salmonella gallinarum, S. pullorum, and S. typhimurium. Infect Immun. 1989 Oct;57(10):3136–3141. doi: 10.1128/iai.57.10.3136-3141.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barrow P. A., Lovell M. A. The association between a large molecular mass plasmid and virulence in a strain of Salmonella pullorum. J Gen Microbiol. 1988 Aug;134(8):2307–2316. doi: 10.1099/00221287-134-8-2307. [DOI] [PubMed] [Google Scholar]
  4. Barrow P. A., Simpson J. M., Lovell M. A., Binns M. M. Contribution of Salmonella gallinarum large plasmid toward virulence in fowl typhoid. Infect Immun. 1987 Feb;55(2):388–392. doi: 10.1128/iai.55.2.388-392.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fierer J., Chikami G., Hatlen L., Heffernan E. J., Guiney D. Active immunization with LD842, a plasmid-cured strain of Salmonella dublin, protects mice against group D and group B Salmonella infection. J Infect Dis. 1988 Aug;158(2):460–463. doi: 10.1093/infdis/158.2.460. [DOI] [PubMed] [Google Scholar]
  6. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nakamura M., Sato S., Ohya T., Suzuki S., Ikeda S., Koeda T. Plasmid-cured Salmonella enteritidis AL1192 as a candidate for a live vaccine. Infect Immun. 1985 Nov;50(2):586–587. doi: 10.1128/iai.50.2.586-587.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SMITH H. W. The use of live vaccines in experimental Salmonella gallinarum infection in chickens with observations on their interference effect. J Hyg (Lond) 1956 Sep;54(3):419–432. doi: 10.1017/s0022172400044685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Silva E. N., Snoeyenbos G. H., Weinack O. M., Smyser C. F. Studies on the use of 9R strain of Salmonella gallinarum as a vaccine in chickens. Avian Dis. 1981 Jan-Mar;25(1):38–52. [PubMed] [Google Scholar]
  10. Smith H. W., Gyles C. L. The relationship between different transmissible plasmids introduced by F into the same strain of Escherichia coli K12. J Gen Microbiol. 1970 Aug;62(3):227–285. doi: 10.1099/00221287-62-3-277. [DOI] [PubMed] [Google Scholar]
  11. Smith H. W., Tucker J. F., Lovell M. Furazolidone resistance in Salmonella gallinarum: the relationship between in vitro and in vivo determinations of resistance. J Hyg (Lond) 1981 Aug;87(1):71–81. doi: 10.1017/s0022172400069254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith I. M. Protection against experimental fowl typhoid by vaccination with strain 9R reconstituted from the freeze-dried state. J Comp Pathol. 1969 Apr;79(2):197–205. doi: 10.1016/0021-9975(69)90006-1. [DOI] [PubMed] [Google Scholar]
  13. Stuart E. E., Keenum R. D., Bruins H. W. Experimental studies on an isolate of Salmonella gallinarum apparently resistant to furazolidone. Avian Dis. 1963 Aug;7(3):294–303. [PubMed] [Google Scholar]
  14. Stuart E. E., Keenum R. D., Bruins H. W. The emergence of a furazolidone-resistant strain of Salmonella gallinarum. Avian Dis. 1967 May;11(2):139–145. [PubMed] [Google Scholar]
  15. Williams Smith H., Tucker J. F. The virulence of salmonella strains for chickens: their excretion by infected chickens. J Hyg (Lond) 1980 Jun;84(3):479–488. doi: 10.1017/s0022172400027017. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES