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. 1990 Aug;58(8):2719–2724. doi: 10.1128/iai.58.8.2719-2724.1990

Synthetic peptide segments from the Escherichia coli porin OmpF constitute leukocyte activators.

H M Vordermeier 1, P Hoffmann 1, F O Gombert 1, G Jung 1, W G Bessler 1
PMCID: PMC258883  PMID: 1695209

Abstract

After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-285) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differentiation into antibody-producing plasma cells and tumor cytotoxicity of macrophages against the fibroblast cell line L929. We thus demonstrated that defined peptide segments are responsible for the leukocyte-activating properties of a major bacterial surface protein.

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Selected References

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