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. 1991 Oct;59(10):3407–3410. doi: 10.1128/iai.59.10.3407-3410.1991

Contribution of the B oligomer to the protective activity of genetically attenuated pertussis toxin.

J L Arciniega 1, R D Shahin 1, W N Burnette 1, T D Bartley 1, D W Whiteley 1, V L Mar 1, D L Burns 1
PMCID: PMC258899  PMID: 1894354

Abstract

An enzymatically deficient recombinant S1 subunit, in which Arg-9 was replaced by Lys, was combined with native B oligomer to form a mutant holotoxin molecule. This molecule exhibited decreased leukocytosis-promoting and histamine-sensitizing activities compared with those of the native toxin, supporting the view that the B oligomer is not responsible for these activities. The protective activity of this genetically attenuated pertussis toxin was compared with that of B oligomer alone. The mutant pertussis toxin and B oligomer were similarly capable of protecting mice against a respiratory infection with Bordetella pertussis, suggesting that the B oligomer makes a significant contribution to the protection afforded by the genetically attenuated holotoxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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