Abstract
This paper presents the first automated system for simultaneously detecting human papilloma, herpes simplex, adenovirus, or cytomegalovirus viral antigens and gene sequences in standard formalin-fixed, paraffin-embedded tissue substrates and tissue culture. These viruses can be detected by colorimetric in situ nucleic acid hybridization, using biotinylated DNA probes, or by indirect immunoperoxidase techniques, using polyclonal or monoclonal antibodies, in a 2.0-hour assay performed at a single automated robotic workstation.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beckmann A. M., Myerson D., Daling J. R., Kiviat N. B., Fenoglio C. M., McDougall J. K. Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes. J Med Virol. 1985 Jul;16(3):265–273. doi: 10.1002/jmv.1890160307. [DOI] [PubMed] [Google Scholar]
- Borisch B., Jahn G., Scholl B. C., Filger-Brillinger J., Heymer B., Fleckenstein B., Müller-Hermelink H. K. Detection of human cytomegalovirus DNA and viral antigens in tissues of different manifestations of CMV infection. Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;55(2):93–99. doi: 10.1007/BF02896564. [DOI] [PubMed] [Google Scholar]
- Brigati D. J., Myerson D., Leary J. J., Spalholz B., Travis S. Z., Fong C. K., Hsiung G. D., Ward D. C. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology. 1983 Apr 15;126(1):32–50. doi: 10.1016/0042-6822(83)90460-9. [DOI] [PubMed] [Google Scholar]
- Kurman R. J., Shah K. H., Lancaster W. D., Jenson A. B. Immunoperoxidase localization of papillomavirus antigens in cervical dysplasia and vulvar condylomas. Am J Obstet Gynecol. 1981 Aug 15;140(8):931–935. doi: 10.1016/0002-9378(81)90087-9. [DOI] [PubMed] [Google Scholar]
- Lack E. E., Jenson A. B., Smith H. G., Healy G. B., Pass F., Vawter G. F. Immunoperoxidase localization of human papillomavirus in laryngeal papillomas. Intervirology. 1980;14(3-4):148–154. doi: 10.1159/000149176. [DOI] [PubMed] [Google Scholar]
- Myerson D., Hackman R. C., Nelson J. A., Ward D. C., McDougall J. K. Widespread presence of histologically occult cytomegalovirus. Hum Pathol. 1984 May;15(5):430–439. doi: 10.1016/s0046-8177(84)80076-3. [DOI] [PubMed] [Google Scholar]
- Pilotti S., Rilke F., Shah K. V., Delle Torre G., De Palo G. Immunohistochemical and ultrastructural evidence of papilloma virus infection associated with in situ and microinvasive squamous cell carcinoma of the vulva. Am J Surg Pathol. 1984 Oct;8(10):751–761. doi: 10.1097/00000478-198410000-00004. [DOI] [PubMed] [Google Scholar]
- Rentrop M., Knapp B., Winter H., Schweizer J. Aminoalkylsilane-treated glass slides as support for in situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions. Histochem J. 1986 May;18(5):271–276. doi: 10.1007/BF01676237. [DOI] [PubMed] [Google Scholar]
- Robey S. S., Gage W. R., Kuhajda F. P. Comparison of immunoperoxidase and DNA in situ hybridization techniques in the diagnosis of cytomegalovirus colitis. Am J Clin Pathol. 1988 May;89(5):666–671. doi: 10.1093/ajcp/89.5.666. [DOI] [PubMed] [Google Scholar]
- Syrjänen S., Partanen P., Mäntyjärvi R., Syrjänen K. Sensitivity of in situ hybridization techniques using biotin- and 35S-labeled human papillomavirus (HPV) DNA probes. J Virol Methods. 1988 Mar-Apr;19(3-4):225–238. doi: 10.1016/0166-0934(88)90017-1. [DOI] [PubMed] [Google Scholar]
- Unger E. R., Budgeon L. R., Myerson D., Brigati D. J. Viral diagnosis by in situ hybridization. Description of a rapid simplified colorimetric method. Am J Surg Pathol. 1986 Jan;10(1):1–8. doi: 10.1097/00000478-198601000-00001. [DOI] [PubMed] [Google Scholar]
- Wilbur D. C., Reichman R. C., Stoler M. H. Detection of infection by human papillomavirus in genital condylomata. A comparison study using immunocytochemistry and in situ nucleic acid hybridization. Am J Clin Pathol. 1988 Apr;89(4):505–510. doi: 10.1093/ajcp/89.4.505. [DOI] [PubMed] [Google Scholar]
- Woodruff J. D., Braun L., Cavalieri R., Gupta P., Pass F., Shah K. V. Immunologic identification of papillomavirus antigen in condyloma tissues from the female genital tract. Obstet Gynecol. 1980 Dec;56(6):727–732. [PubMed] [Google Scholar]