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. 1991 Jan-Feb;64(1):89–102.

Measurement of the rate of aqueous humor flow.

S D Smith 1
PMCID: PMC2589442  PMID: 1897265

Abstract

Techniques which estimate the rate of aqueous flow generally require the use of tracer substances. Determination of the distribution of the tracer in the relevant body compartments permits calculation of the rate of flow within the limits of accuracy of the method used. The underlying theory, as well as the advantages and limitations of methods employing systemic, topical, intracameral, and intravitreal administration of tracer substances are reviewed. Since these methods all assume that the rate of aqueous secretion is constant, yet the presence of a diurnal rhythm of flow has been demonstrated in both rabbits and humans, a compartmental model of a circadian system based upon the vitreous depot technique is presented. This model estimates the degree to which a continuously changing rate of aqueous flow limits the ability to determine aqueous flow rate accurately by this particular method and illustrates this limitation, which is common to all tracer methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANJOU C. I., KRAKAU C. E. Aqueous flare and protein content in the anterior chamber of normal rabbits' eyes. Acta Ophthalmol (Copenh) 1961;39:95–101. doi: 10.1111/j.1755-3768.1961.tb00244.x. [DOI] [PubMed] [Google Scholar]
  2. ANJOU C. I. Physiological variations of the aqueous flare density in normal human eyes. Acta Ophthalmol (Copenh) 1961;39:525–539. doi: 10.1111/j.1755-3768.1961.tb00882.x. [DOI] [PubMed] [Google Scholar]
  3. BARANY E., KINSEY V. E. The rate of flow of aqueous humor; the rate of disappearance of para-aminohippuric acid, radioactive rayopake, and radioactive diodrast from the aqueous humor of rabbits. Am J Ophthalmol. 1949 Jun;32(Pt 2)(6):177–188. [PubMed] [Google Scholar]
  4. BECKER B. The measurement of rate of aqueous flow with iodide. Invest Ophthalmol. 1962 Feb;1:52–58. [PubMed] [Google Scholar]
  5. Bill A. The aqueous humor drainage mechanism in the cynomolgus monkey (Macaca irus) with evidence for unconventional routes. Invest Ophthalmol. 1965 Oct;4(5):911–919. [PubMed] [Google Scholar]
  6. Coakes R. L., Brubaker R. F. Method of measuring aqueous humor flow and corneal endothelial permeability using a fluorophotometry nomogram. Invest Ophthalmol Vis Sci. 1979 Mar;18(3):288–302. [PubMed] [Google Scholar]
  7. Gaul G. R., Brubaker R. F. Measurement of aqueous flow in rabbits with corneal and vitreous depots of fluorescent dye. Invest Ophthalmol Vis Sci. 1986 Sep;27(9):1331–1335. [PubMed] [Google Scholar]
  8. Johnson F., Maurice D. A simple method of measuring aqueous humor flow with intravitreal fluoresceinated dextrans. Exp Eye Res. 1984 Dec;39(6):791–805. doi: 10.1016/0014-4835(84)90078-2. [DOI] [PubMed] [Google Scholar]
  9. Jones R. F., Maurice D. M. New methods of measuring the rate of aqueous flow in man with fluorescein. Exp Eye Res. 1966 Jul;5(3):208–220. doi: 10.1016/s0014-4835(66)80009-x. [DOI] [PubMed] [Google Scholar]
  10. KINSEY V. E., BARANY E. The rate of flow of aqueous humor; derivation of rate of flow and its physiologic significance. Am J Ophthalmol. 1949 Jun;32(Pt 2)(6):189–202. [PubMed] [Google Scholar]
  11. KRAKAU C. E. On the connection between aqueous flow and flare. Ophthalmologica. 1962;144:153–164. doi: 10.1159/000304317. [DOI] [PubMed] [Google Scholar]
  12. Kinsey V. E., Grant W. M. THE MECHANISM OF AQUEOUS HUMOR FORMATION INFERRED FROM CHEMICAL STUDIES ON BLOOD-AQUEOUS HUMOR DYNAMICS. J Gen Physiol. 1942 Nov 20;26(2):131–149. doi: 10.1085/jgp.26.2.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kottler M., Brubaker R., Macri F. The decay kinetics of substances which leave the anterior chamber by bulk flow. Invest Ophthalmol. 1970 Oct;9(10):758–764. [PubMed] [Google Scholar]
  14. Krakau C. E. A photoelectric method for estimating the pupillary flow of aqueous humour. Experientia. 1967 Nov 15;23(11):928–929. doi: 10.1007/BF02136226. [DOI] [PubMed] [Google Scholar]
  15. LANGLEY D., MACDONALD R. K. Clinical method of observing changes in the rate of flow of aqueous humour in the human eye. I. Normal eyes. Br J Ophthalmol. 1952 Aug;36(8):432–437. doi: 10.1136/bjo.36.8.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LINNER E. Ascorbic acid as a test substance for measuring relative changes in the rate of plasma flow through the ciliary processes. I. The effect of unilateral ligation of the common carotid artery in rabbits on the ascorbic acid content of the aqueous humour at varying plasma levels. Acta Physiol Scand. 1952 Jul 17;26(1):57–69. doi: 10.1111/j.1748-1716.1952.tb00891.x. [DOI] [PubMed] [Google Scholar]
  17. Macri F. J., O'Rourke J. Measurements of aqueous humor turnover rates using a gamma probe. Arch Ophthalmol. 1970 Jun;83(6):741–745. doi: 10.1001/archopht.1970.00990030741013. [DOI] [PubMed] [Google Scholar]
  18. McLaren J. W., Trocme S. D., Relf S., Brubaker R. F. Rate of flow of aqueous humor determined from measurements of aqueous flare. Invest Ophthalmol Vis Sci. 1990 Feb;31(2):339–346. [PubMed] [Google Scholar]
  19. O'Rourke J., Macri F. J., Berghoffer B. Studies in uveal physiology. I. Adaptation of isotope clearance procedures for external monitoring of anterior uveal bloodflow and aqueous humor turnover in the dog. Arch Ophthalmol. 1969 Apr;81(4):526–533. doi: 10.1001/archopht.1969.00990010528011. [DOI] [PubMed] [Google Scholar]
  20. Oppelt W. W. Measurement of aqueous humor formation rates by posterior-anterior chamber perfusion with inulin: normal values and the effect of carbonic anhydrase inhibition. Invest Ophthalmol. 1967 Feb;6(1):76–83. [PubMed] [Google Scholar]
  21. Oshika T., Araie M., Masuda K. Diurnal variation of aqueous flare in normal human eyes measured with laser flare-cell meter. Jpn J Ophthalmol. 1988;32(2):143–150. [PubMed] [Google Scholar]
  22. Reiss G. R., Lee D. A., Topper J. E., Brubaker R. F. Aqueous humor flow during sleep. Invest Ophthalmol Vis Sci. 1984 Jun;25(6):776–778. [PubMed] [Google Scholar]
  23. Smith S. D., Gregory D. S. A circadian rhythm of aqueous flow underlies the circadian rhythm of IOP in NZW rabbits. Invest Ophthalmol Vis Sci. 1989 Apr;30(4):775–778. [PubMed] [Google Scholar]
  24. Sperber G. O., Bill A. A method for near-continuous determination of aqueous humor flow; effects of anaesthetics, temperature and indomethacin. Exp Eye Res. 1984 Oct;39(4):435–453. doi: 10.1016/0014-4835(84)90044-7. [DOI] [PubMed] [Google Scholar]
  25. Topper J. E., McLaren J., Brubaker R. F. Measurement of aqueous humor flow with scanning ocular fluorophotometers. Curr Eye Res. 1984 Dec;3(12):1391–1395. doi: 10.3109/02713688409000834. [DOI] [PubMed] [Google Scholar]

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