Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1991 Oct;59(10):3750–3753. doi: 10.1128/iai.59.10.3750-3753.1991

Characterization of bradyzoite-specific antigens of Toxoplasma gondii.

S Tomavo 1, B Fortier 1, M Soete 1, C Ansel 1, D Camus 1, J F Dubremetz 1
PMCID: PMC258946  PMID: 1894373

Abstract

Monoclonal antibodies that react specifically with bradyzoite antigens of Toxoplasma gondii were selected by differential immunofluorescence among hybridomas produced against these organisms. These antigens were further characterized by immunofluorescence on living bradyzoites and Western immunoblotting. Four pellicular antigens (36, 34, 21, and 18 kDa) were identified; three of these are exposed on the surface of the organism and accessible to either antibodies, trypsin cleavage, or both of these surface-probing procedures. These antigens were found on recent human isolates of T. gondii, as well as on laboratory strain bradyzoites obtained from either brain cysts or in vitro-grown parasites.

Full text

PDF
3750

Images in this article

3751Image
on p.3751
3752Image
on p.3752

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cornelissen A. W., Overdulve J. P., Hoenderboom J. M. Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissue by continuous density-gradient centrifugation. Parasitology. 1981 Aug;83(Pt 1):103–108. doi: 10.1017/s0031182000050071. [DOI] [PubMed] [Google Scholar]
  2. Couvreur G., Sadak A., Fortier B., Dubremetz J. F. Surface antigens of Toxoplasma gondii. Parasitology. 1988 Aug;97(Pt 1):1–10. doi: 10.1017/s0031182000066695. [DOI] [PubMed] [Google Scholar]
  3. Couzineau P., Baufine-Ducrocq H. Etude des possibilités d'utilisation du sarcome TG 180 de la souris. Application à la toxoplasmose. Ann Parasitol Hum Comp. 1969 May-Jun;44(3):217–224. [PubMed] [Google Scholar]
  4. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  5. Grimwood B. G., Hechemy K., Stevens R. W. Toxoplasma gondii: purification of trophozoites propagated in cell culture. Exp Parasitol. 1979 Oct;48(2):282–286. doi: 10.1016/0014-4894(79)90110-3. [DOI] [PubMed] [Google Scholar]
  6. Handman E., Goding J. W., Remington J. S. Detection and characterization of membrane antigens of Toxoplasma gondii. J Immunol. 1980 Jun;124(6):2578–2583. [PubMed] [Google Scholar]
  7. Jones T. C., Bienz K. A., Erb P. In vitro cultivation of Toxoplasma gondii cysts in astrocytes in the presence of gamma interferon. Infect Immun. 1986 Jan;51(1):147–156. doi: 10.1128/iai.51.1.147-156.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kasper L. H., Bradley M. S., Pfefferkorn E. R. Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J Immunol. 1984 Jan;132(1):443–449. [PubMed] [Google Scholar]
  9. Kasper L. H. Identification of stage-specific antigens of Toxoplasma gondii. Infect Immun. 1989 Mar;57(3):668–672. doi: 10.1128/iai.57.3.668-672.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Laugier M., Quilici M. Intérêt expérimental d'une souche de Toxoplasme peu pathogène pour la souris. Ann Parasitol Hum Comp. 1970 Jul-Aug;45(4):389–403. [PubMed] [Google Scholar]
  12. Lunde M. N., Jacobs L. Antigenic differences between endozoites and cystozoites of Toxoplasma gondii. J Parasitol. 1983 Oct;69(5):806–808. [PubMed] [Google Scholar]
  13. Nagel S. D., Boothroyd J. C. The major surface antigen, P30, of Toxoplasma gondii is anchored by a glycolipid. J Biol Chem. 1989 Apr 5;264(10):5569–5574. [PubMed] [Google Scholar]
  14. Omata Y., Igarashi M., Ramos M. I., Nakabayashi T. Toxoplasma gondii: antigenic differences between endozoites and cystozoites defined by monoclonal antibodies. Parasitol Res. 1989;75(3):189–193. doi: 10.1007/BF00931274. [DOI] [PubMed] [Google Scholar]
  15. Sharma S. P., Dubey J. P. Quantitative survival of Toxoplasma gondii tachyzoites and bradyzoites in pepsin and in trypsin solutions. Am J Vet Res. 1981 Jan;42(1):128–130. [PubMed] [Google Scholar]
  16. Shimada K., O'Connor G. R., Yoneda C. Cyst formation by Toxoplasma gondii (RH strain) in vitro. The role of immunologic mechanisms. Arch Ophthalmol. 1974 Dec;92(6):496–500. doi: 10.1001/archopht.1974.01010010510010. [DOI] [PubMed] [Google Scholar]
  17. Tomavo S., Schwarz R. T., Dubremetz J. F. Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens. Mol Cell Biol. 1989 Oct;9(10):4576–4580. doi: 10.1128/mcb.9.10.4576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES