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. 1991 Oct;59(10):3819–3822. doi: 10.1128/iai.59.10.3819-3822.1991

Effects of temperature, amebic strain, and carbohydrates on Acanthamoeba adherence to corneal epithelium in vitro.

L D Morton 1, G L McLaughlin 1, H E Whiteley 1
PMCID: PMC258957  PMID: 1894379

Abstract

An in vitro coincubation assay was used to measure adhesion of radiolabeled Acanthamoeba trophozoites to corneal epithelium. Adhesion of amebae to corneal epithelium was higher at 25 degrees C than at 37 or 4 degrees C, did not consistently correlate with the reported pathogenicity of the strain of Acanthamoeba, and was inhibited by mannose and by methyl-alpha-D-mannopyranoside.

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Selected References

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  1. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. doi: 10.1093/infdis/143.3.325. [DOI] [PubMed] [Google Scholar]
  2. Butrus S. I., Klotz S. A., Misra R. P. The adherence of Pseudomonas aeruginosa to soft contact lenses. Ophthalmology. 1987 Oct;94(10):1310–1314. doi: 10.1016/s0161-6420(87)80017-9. [DOI] [PubMed] [Google Scholar]
  3. De Jonckheere J. F. Growth characteristics, cytopathic effect in cell culture, and virulence in mice of 36 type strains belonging to 19 different Acanthamoeba spp. Appl Environ Microbiol. 1980 Apr;39(4):681–685. doi: 10.1128/aem.39.4.681-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Efron N., Young G., Brennan N. A. Ocular surface temperature. Curr Eye Res. 1989 Sep;8(9):901–906. [PubMed] [Google Scholar]
  5. Fowler S. A., Allansmith M. R. Evolution of soft contact lens coatings. Arch Ophthalmol. 1980 Jan;98(1):95–99. doi: 10.1001/archopht.1980.01020030097007. [DOI] [PubMed] [Google Scholar]
  6. Hazlett L. D., Moon M., Berk R. S. In vivo identification of sialic acid as the ocular receptor for Pseudomonas aeruginosa. Infect Immun. 1986 Feb;51(2):687–689. doi: 10.1128/iai.51.2.687-689.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. John T., Desai D., Sahm D. Adherence of Acanthamoeba castellanii cysts and trophozoites to unworn soft contact lenses. Am J Ophthalmol. 1989 Dec 15;108(6):658–664. doi: 10.1016/0002-9394(89)90857-x. [DOI] [PubMed] [Google Scholar]
  8. Jumblatt M. M., Neufeld A. H. A tissue culture assay of corneal epithelial wound closure. Invest Ophthalmol Vis Sci. 1986 Jan;27(1):8–13. [PubMed] [Google Scholar]
  9. Klotz S. A., Misra R. P., Butrus S. I. Carbohydrate deposits on the surfaces of worn extended-wear soft contact lenses. Arch Ophthalmol. 1987 Jul;105(7):974–977. doi: 10.1001/archopht.1987.01060070118039. [DOI] [PubMed] [Google Scholar]
  10. Klotz S. A., Misra R. P., Butrus S. I. Contact lens wear enhances adherence of Pseudomonas aeruginosa and binding of lectins to the cornea. Cornea. 1990 Jul;9(3):266–270. [PubMed] [Google Scholar]
  11. McLaughlin G. L., Stimac J. E., Luke J. M., Kuhlenschmidt M. S., Vernon R. A., Morton L. D., Visvesvara G. S., Whiteley H. E. Development of Acanthamoeba-cornea coincubation assays. Rev Infect Dis. 1991 Mar-Apr;13 (Suppl 5):S397–S398. doi: 10.1093/clind/13.supplement_5.s397. [DOI] [PubMed] [Google Scholar]
  12. Morton L. D., McLaughlin G. L., Whiteley H. E. Adherence characteristics of three strains of Acanthamoeba. Rev Infect Dis. 1991 Mar-Apr;13 (Suppl 5):S424–S424. doi: 10.1093/clind/13.supplement_5.s424. [DOI] [PubMed] [Google Scholar]
  13. Paatero G. I., Gahmberg C. G. Detection of glycoproteins in the Acanthamoeba plasma membrane. Exp Cell Res. 1988 Nov;179(1):253–262. doi: 10.1016/0014-4827(88)90364-3. [DOI] [PubMed] [Google Scholar]
  14. Petri W. A., Jr, Jackson T. F., Gathiram V., Kress K., Saffer L. D., Snodgrass T. L., Chapman M. D., Keren Z., Mirelman D. Pathogenic and nonpathogenic strains of Entamoeba histolytica can be differentiated by monoclonal antibodies to the galactose-specific adherence lectin. Infect Immun. 1990 Jun;58(6):1802–1806. doi: 10.1128/iai.58.6.1802-1806.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ravdin J. I. Entamoeba histolytica: from adherence to enteropathy. J Infect Dis. 1989 Mar;159(3):420–429. doi: 10.1093/infdis/159.3.420. [DOI] [PubMed] [Google Scholar]
  16. Spurr-Michaud S. J., Barza M., Gipson I. K. An organ culture system for study of adherence of Pseudomonas aeruginosa to normal and wounded corneas. Invest Ophthalmol Vis Sci. 1988 Mar;29(3):379–386. [PubMed] [Google Scholar]
  17. Su H., Watkins N. G., Zhang Y. X., Caldwell H. D. Chlamydia trachomatis-host cell interactions: role of the chlamydial major outer membrane protein as an adhesin. Infect Immun. 1990 Apr;58(4):1017–1025. doi: 10.1128/iai.58.4.1017-1025.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sun T. T., Shih C., Green H. Keratin cytoskeletons in epithelial cells of internal organs. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2813–2817. doi: 10.1073/pnas.76.6.2813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tjia K. F., van Putten J. P., Pels E., Zanen H. C. The interaction between Neisseria gonorrhoeae and the human cornea in organ culture. An electron microscopic study. Graefes Arch Clin Exp Ophthalmol. 1988;226(4):341–345. doi: 10.1007/BF02172964. [DOI] [PubMed] [Google Scholar]
  20. Visvesvara G. S., Balamuth W. Comparative studies on related free-living and pathogenic amebae with special reference to Acanthamoeba. J Protozool. 1975 May;22(2):245–256. doi: 10.1111/j.1550-7408.1975.tb05860.x. [DOI] [PubMed] [Google Scholar]
  21. Zieske J. D., Higashijima S. C., Gipson I. K. Con A- and WGA-binding glycoproteins of stationary and migratory corneal epithelium. Invest Ophthalmol Vis Sci. 1986 Aug;27(8):1205–1210. [PubMed] [Google Scholar]

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