Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1991 Nov;59(11):3909–3916. doi: 10.1128/iai.59.11.3909-3916.1991

Antibody recognition and isoelectrofocusing of antigens of the malaria parasite Plasmodium yoelii.

F A Kironde 1, A Kumar 1, A R Nayak 1, J L Kraikov 1
PMCID: PMC258976  PMID: 1937750

Abstract

Inbred BALB/c mice were either immunized with Triton X-100-extracted antigens of blood-stage Plasmodium yoelii or infected with P. yoelii and cured in three successive schedules. Whereas the immunized BALB/c became only partially protected from subsequent challenge infection with blood-stage P. yoelii, the convalescent mice acquired total immunity. When total P. yoelii antigen extract was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and immunoblotted with anti-P. yoelii serum, five major protein bands of 150, 84, 40, 19, and 16 kDa were recognized by the sera of fully protected convalescent mice but not by the sera of partially protected mice. The utility of comparing reactivities of sera from fully protected and partially protected malaria hosts and the possibility that antigens uniquely recognized by the convalescent mouse sera may contribute to immunity against P. yoelii infection are discussed. Although previously reported to be an effective adjuvant for immunization against P. yoelii infection in (BALB/c x C57BL)F1 hybrid mice, saponin did not promote protection any better than did Freund adjuvant in BALB/c mice immunized with detergent-extracted P. yoelii antigen. Most of the P. yoelii proteins (14 to 250 kDa) found in Triton X-100 extracts of P. yoelii-parasitized erythrocytes isoelectrofocused as a single peak in the pH region 4.4 to 4.6, suggesting a rationale for previous findings that the most anti-P. yoelii protective and T-helper activities are induced by antigens isoelectrically focused in a fraction of similar pH.

Full text

PDF
3909

Images in this article

3913Image
on p.3913
3914Image
on p.3914
3914Image
on p.3914

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anders R. F., Coppel R. L., Brown G. V., Kemp D. J. Antigens with repeated amino acid sequences from the asexual blood stages of Plasmodium falciparum. Prog Allergy. 1988;41:148–172. [PubMed] [Google Scholar]
  2. Bouharoun-Tayoun H., Attanath P., Sabchareon A., Chongsuphajaisiddhi T., Druilhe P. Antibodies that protect humans against Plasmodium falciparum blood stages do not on their own inhibit parasite growth and invasion in vitro, but act in cooperation with monocytes. J Exp Med. 1990 Dec 1;172(6):1633–1641. doi: 10.1084/jem.172.6.1633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Certa U., Ghersa P., Döbeli H., Matile H., Kocher H. P., Shrivastava I. K., Shaw A. R., Perrin L. H. Aldolase activity of a Plasmodium falciparum protein with protective properties. Science. 1988 May 20;240(4855):1036–1038. doi: 10.1126/science.3285469. [DOI] [PubMed] [Google Scholar]
  4. De Souza J. B., Playfair J. H. Immunization of mice against blood-stage Plasmodium yoelii malaria with isoelectrically focused antigens and correlation of immunity with T-cell priming in vivo. Infect Immun. 1988 Jan;56(1):88–91. doi: 10.1128/iai.56.1.88-91.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FULTON J. D., GRANT P. T. The sulphur requirements of the erythrocytic from of Plasmodium knowlesi. Biochem J. 1956 Jun;63(2):274–282. doi: 10.1042/bj0630274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fenton B., Walker A., Walliker D. Protein variation in clones of Plasmodium falciparum detected by two dimensional electrophoresis. Mol Biochem Parasitol. 1985 Aug;16(2):173–183. doi: 10.1016/0166-6851(85)90085-4. [DOI] [PubMed] [Google Scholar]
  7. Groux H., Gysin J. Opsonization as an effector mechanism in human protection against asexual blood stages of Plasmodium falciparum: functional role of IgG subclasses. Res Immunol. 1990 Jul-Aug;141(6):529–542. doi: 10.1016/0923-2494(90)90021-p. [DOI] [PubMed] [Google Scholar]
  8. Herrera S., Herrera M. A., Perlaza B. L., Burki Y., Caspers P., Döbeli H., Rotmann D., Certa U. Immunization of Aotus monkeys with Plasmodium falciparum blood-stage recombinant proteins. Proc Natl Acad Sci U S A. 1990 May;87(10):4017–4021. doi: 10.1073/pnas.87.10.4017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holder A. A. The precursor to major merozoite surface antigens: structure and role in immunity. Prog Allergy. 1988;41:72–97. [PubMed] [Google Scholar]
  10. Homewood C. A., Neame K. D. A comparison of methods used for the removal of white cells from malaria-infected blood. Ann Trop Med Parasitol. 1976 Jun;70(2):249–251. doi: 10.1080/00034983.1976.11687119. [DOI] [PubMed] [Google Scholar]
  11. Jayawardena A. N., Targett G. A., Leuchars E., Davies A. J. The immunological response of CBA mice to P. yoelii. II. The passive transfer of immunity with serum and cells. Immunology. 1978 Jan;34(1):157–165. [PMC free article] [PubMed] [Google Scholar]
  12. Kemp D. J., Coppel R. L., Stahl H. D., Bianco A. E., Corcoran L. M., McIntyre P., Langford C. J., Favaloro J. M., Crewther P. E., Brown G. V. The Wellcome Trust lecture. Genes for antigens of Plasmodium falciparum. Parasitology. 1986;92 (Suppl):S83–108. doi: 10.1017/s0031182000085711. [DOI] [PubMed] [Google Scholar]
  13. LaRochelle W. J., Froehner S. C. Immunochemical detection of proteins biotinylated on nitrocellulose replicas. J Immunol Methods. 1986 Aug 21;92(1):65–71. doi: 10.1016/0022-1759(86)90504-1. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Miller L. H., Howard R. J., Carter R., Good M. F., Nussenzweig V., Nussenzweig R. S. Research toward malaria vaccines. Science. 1986 Dec 12;234(4782):1349–1356. doi: 10.1126/science.2431481. [DOI] [PubMed] [Google Scholar]
  16. Mitchell G. H. An update on candidate malaria vaccines. Parasitology. 1989;98 (Suppl):S29–S47. doi: 10.1017/s0031182000072231. [DOI] [PubMed] [Google Scholar]
  17. Ozaki L. S., Mattei D., Jendoubi M., Druihle P., Blisnick T., Guillotte M., Puijalon O., Da Silva L. P. Plaque antibody selection: rapid immunological analysis of a large number of recombinant phage clones positive to sera raised against Plasmodium falciparum antigens. J Immunol Methods. 1986 May 22;89(2):213–219. doi: 10.1016/0022-1759(86)90360-1. [DOI] [PubMed] [Google Scholar]
  18. Perrin L. H., Merkli B., Gabra M. S., Stocker J. W., Chizzolini C., Richle R. Immunization with a Plasmodium falciparum merozoite surface antigen induces a partial immunity in monkeys. J Clin Invest. 1985 May;75(5):1718–1721. doi: 10.1172/JCI111881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  20. Playfair J. H., De Souza J. B. Vaccination of mice against malaria with soluble antigens. I. The effect of detergent, route of injection, and adjuvant. Parasite Immunol. 1986 Sep;8(5):409–414. doi: 10.1111/j.1365-3024.1986.tb00857.x. [DOI] [PubMed] [Google Scholar]
  21. Saul A. Kinetic constraints on the development of a malaria vaccine. Parasite Immunol. 1987 Jan;9(1):1–9. doi: 10.1111/j.1365-3024.1987.tb00483.x. [DOI] [PubMed] [Google Scholar]
  22. Tait A. Analysis of protein variation in Plasmodium falciparum by two-dimensional gel electrophoresis. Mol Biochem Parasitol. 1981 Feb;2(3-4):205–218. doi: 10.1016/0166-6851(81)90101-8. [DOI] [PubMed] [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Troye-Blomberg M., Perlmann P. T cell functions in Plasmodium falciparum and other malarias. Prog Allergy. 1988;41:253–287. doi: 10.1159/000415226. [DOI] [PubMed] [Google Scholar]
  25. Yoeli M., Hargreaves B., Carter R., Walliker D. Sudden increase in virulence in a strain of Plasmodium berghei yoelii. Ann Trop Med Parasitol. 1975 Jun;69(2):173–178. doi: 10.1080/00034983.1975.11686998. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES