Skip to main content
NCBI home page
The Yale Journal of Biology and Medicine logoLink to The Yale Journal of Biology and Medicine
. 1985 Mar-Apr;58(2):125–131.

Changes in membrane gangliosides: differentiation of human and murine monocytic cells.

J L Ryan, H C Yohe, H L Malech
PMCID: PMC2589882  PMID: 3862286

Abstract

Membrane ganglioside changes in murine peritoneal macrophages and the human promyelocytic leukemia cell line HL-60 have been assessed by two-dimensional thin-layer chromatography. C3H/HeJ mice respond to protein-containing endotoxin but are hyporesponsive to protein-free endotoxin preparations. Compared to unstimulated resident cells, protein-containing endotoxin produced an alteration in the C3H/HeJ macrophage ganglioside pattern whereas protein-free endotoxin did not. In comparison, differentiation of HL-60 cells to a neutrophil-like cell by dimethylsulfoxide gave a ganglioside pattern similar to unstimulated HL-60 cells. However, differentiation of HL-60 cells by phorbol myristate acetate to macrophage-like cells results in a large increase in the monosialoganglioside GM3. The evidence presented indicates that discrete ganglioside changes occur in murine monocytes and HL-60 cells upon induction to cells with increased macrophage functions.

Full text

PDF
125

Images in this article

128FIG. 1
on p.128
129FIG. 2
on p.129

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander P., Evans R. Endotoxin and double stranded RNA render macrophages cytotoxic. Nat New Biol. 1971 Jul 21;232(29):76–78. doi: 10.1038/newbio232076a0. [DOI] [PubMed] [Google Scholar]
  2. Dinarello C. A. Interleukin-1. Rev Infect Dis. 1984 Jan-Feb;6(1):51–95. doi: 10.1093/clinids/6.1.51. [DOI] [PubMed] [Google Scholar]
  3. Fishman P. H. Role of membrane gangliosides in the binding and action of bacterial toxins. J Membr Biol. 1982;69(2):85–97. doi: 10.1007/BF01872268. [DOI] [PubMed] [Google Scholar]
  4. Glode L. M., Mergenhagen S. E., Rosenstreich D. L. Significant contribution of spleen cells in mediating the lethal effects of endotoxin in vivo. Infect Immun. 1976 Sep;14(3):626–630. doi: 10.1128/iai.14.3.626-630.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kohn L. D., Consiglio E., DeWolf M. J., Grollman E. F., Ledley F. D., Lee G., Morris N. P. Thyrotropin receptors and gangliosides. Adv Exp Med Biol. 1980;125:487–503. doi: 10.1007/978-1-4684-7844-0_43. [DOI] [PubMed] [Google Scholar]
  6. Ledeen R. W., Haley J. E., Skrivanek J. A. Study of ganglioside patterns with two-dimensional and thin-layer chromatography and radioautography; detection of new fucogangliosides and other minor species in rabbit brain. Anal Biochem. 1981 Mar 15;112(1):135–142. doi: 10.1016/0003-2697(81)90270-0. [DOI] [PubMed] [Google Scholar]
  7. McIntire F. C., Sievert H. W., Barlow G. H., Finley R. A., Lee A. Y. Chemical, physical, biological properties of a lipopolysaccharide from Escherichia coli K-235. Biochemistry. 1967 Aug;6(8):2363–2372. doi: 10.1021/bi00860a011. [DOI] [PubMed] [Google Scholar]
  8. Morrison D. C., Betz S. J., Jacobs D. M. Isolation of a lipid A bound polypeptide responsible for "LPS-initiated" mitogenesis of C3H/HeJ spleen cells. J Exp Med. 1976 Sep 1;144(3):840–846. doi: 10.1084/jem.144.3.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morrison D. C., Leive L. Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures. J Biol Chem. 1975 Apr 25;250(8):2911–2919. [PubMed] [Google Scholar]
  10. Morrison D. C., Ulevitch R. J. The effects of bacterial endotoxins on host mediation systems. A review. Am J Pathol. 1978 Nov;93(2):526–618. [PMC free article] [PubMed] [Google Scholar]
  11. Nathan C. F., Root R. K. Hydrogen peroxide release from mouse peritoneal macrophages: dependence on sequential activation and triggering. J Exp Med. 1977 Dec 1;146(6):1648–1662. doi: 10.1084/jem.146.6.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nojiri H., Takaku F., Tetsuka T., Motoyoshi K., Miura Y., Saito M. Characteristic expression of glycosphingolipid profiles in the bipotential cell differentiation of human promyelocytic leukemia cell line HL-60. Blood. 1984 Aug;64(2):534–541. [PubMed] [Google Scholar]
  13. Ryan J. L., Yohe W. B. Lymphocyte mediation of lipopolysaccharide-stimulated macrophage metabolism. J Immunol. 1981 Sep;127(3):912–916. [PubMed] [Google Scholar]
  14. SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. doi: 10.1016/0006-3002(57)90254-8. [DOI] [PubMed] [Google Scholar]
  15. Skidmore B. J., Morrison D. C., Chiller J. M., Weigle W. O. Immunologic properties of bacterial lipopolysaccharide (LPS). II. The unresponsiveness of C3H/HeJ Mouse spleen cells to LPS-induced mitogenesis is dependent on the method used to extract LPS. J Exp Med. 1975 Dec 1;142(6):1488–1508. doi: 10.1084/jem.142.6.1488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stein-Douglas K., Schwarting G. A., Naiki M., Marcus D. M. Gangliosides as markers for murine lymphocyte subpopulations. J Exp Med. 1976 Apr 1;143(4):822–832. doi: 10.1084/jem.143.4.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vengris V. E., Reynolds F. H., Jr, Hollenberg M. D., Pitha P. M. Interferon action: role of membrane gangliosides. Virology. 1976 Jul 15;72(2):486–493. doi: 10.1016/0042-6822(76)90177-x. [DOI] [PubMed] [Google Scholar]
  18. Yohe H. C., Yu R. K. In vitro biosynthesis of an isomer of brain trisialoganglioside, GT1a. J Biol Chem. 1980 Jan 25;255(2):608–613. [PubMed] [Google Scholar]

Articles from The Yale Journal of Biology and Medicine are provided here courtesy of Yale Journal of Biology and Medicine

RESOURCES