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. 1991 Nov;59(11):4201–4211. doi: 10.1128/iai.59.11.4201-4211.1991

Cytopathic effects of Mycoplasma pulmonis in vivo and in vitro.

C T Stadtländer 1, H L Watson 1, J W Simecka 1, G H Cassell 1
PMCID: PMC259017  PMID: 1937777

Abstract

This study was performed to evaluate the cytopathic features resulting from Mycoplasma pulmonis infection of tracheal organ cultures compared to with those seen in in vivo infection and to use this system to determine possible differences in cytopathic effects in two M. pulmonis variants found to cause different diseases in vivo. The attachment of M. pulmonis to respiratory epithelium was similar in vivo and in vitro. Cytopathic effects seen in both systems were also similar in loss of tight junctions between cells and exfoliation of respiratory cells, resulting in exposure of the subepithelial layer. These similarities indicate that the observed tissue damage is initiated by the mycoplasmas rather than by immunologic host responses but does not exclude the possibility that host responses may subsequently contribute to the cytopathological events. Comparison of the effects of the two variants (one known to cause death in vivo) did not reveal differences in vitro. This suggests that host factors (not present in vitro) may account for differences in virulence. Detailed in vitro studies allowed the identification of the time frame corresponding to the in vivo infection and also revealed the limitations of the in vitro system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albers A. C., Fletcher R. D. Simple method for quantitation of viable mycoplasmas. Appl Environ Microbiol. 1982 Apr;43(4):958–960. doi: 10.1128/aem.43.4.958-960.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Araake M. Comparison of ciliostasis by mycoplasmas in mouse and chicken tracheal organ cultures. Microbiol Immunol. 1982;26(1):1–14. doi: 10.1111/j.1348-0421.1982.tb00148.x. [DOI] [PubMed] [Google Scholar]
  3. Araake M., Yayoshi M., Yoshioka M. Electron microscopic studies on the attachment of Mycoplasma pulmonis to mouse synovial cells cultured in vitro. Microbiol Immunol. 1984;28(3):379–384. doi: 10.1111/j.1348-0421.1984.tb00689.x. [DOI] [PubMed] [Google Scholar]
  4. Collier A. M., Clyde W. A. Relationships Between Mycoplasma pneumoniae and Human Respiratory Epithelium. Infect Immun. 1971 May;3(5):694–701. doi: 10.1128/iai.3.5.694-701.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis J. K., Thorp R. B., Parker R. F., White H., Dziedzic D., D'Arcy J., Cassell G. H. Development of an aerosol model of murine respiratory mycoplasmosis in mice. Infect Immun. 1986 Oct;54(1):194–201. doi: 10.1128/iai.54.1.194-201.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dybvig K., Alderete J., Watson H. L., Cassell G. H. Adsorption of mycoplasma virus P1 to host cells. J Bacteriol. 1988 Sep;170(9):4373–4375. doi: 10.1128/jb.170.9.4373-4375.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dybvig K., Simecka J. W., Watson H. L., Cassell G. H. High-frequency variation in Mycoplasma pulmonis colony size. J Bacteriol. 1989 Sep;171(9):5165–5168. doi: 10.1128/jb.171.9.5165-5168.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Finlay B. B., Heffron F., Falkow S. Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion. Science. 1989 Feb 17;243(4893):940–943. doi: 10.1126/science.2919285. [DOI] [PubMed] [Google Scholar]
  10. Friis N. F. Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey. Nord Vet Med. 1975 Jun;27(6):337–339. [PubMed] [Google Scholar]
  11. Gabridge M. G., Barden-Stahl Y. D., Polisky R. B., Engelhardt J. A. Differences in the attachment of Mycoplasma pneumoniae cells and membranes to tracheal epithelium. Infect Immun. 1977 Jun;16(3):766–772. doi: 10.1128/iai.16.3.766-772.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gabridge M. G., Johnson C. K., Cameron A. M. Cytotoxicity of Mycoplasma pneumoniae Membranes. Infect Immun. 1974 Nov;10(5):1127–1134. doi: 10.1128/iai.10.5.1127-1134.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KRAMER H., WINDRUM G. M. The metachromatic staining reaction. J Histochem Cytochem. 1955 May;3(3):227–237. doi: 10.1177/3.3.227. [DOI] [PubMed] [Google Scholar]
  14. Kirchhoff H., Heitmann J., Ammar A., Hermanns W., Schulz L. C. Studies of polyarthritis caused by Mycoplasma arthritidis in rats. I. Detection of the persisting Mycoplasma antigen by the enzyme immune assay (EIA) and conventional culture technique. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Mar;254(1):129–138. [PubMed] [Google Scholar]
  15. Kohn D. F. Bronchiectasis in rats infected with Mycoplasma pulmonis: an electron microscopy study. Lab Anim Sci. 1971 Dec;21(6):856–861. [PubMed] [Google Scholar]
  16. Organick A. B., Siegesmund K. A., Lutsky I. I. Pneumonia due to mycoplasma in gnotobiotic mice. II. Localization of Mycoplasma pulmonis in the lungs of infected gnotobiotic mice by electron microscopy. J Bacteriol. 1966 Oct;92(4):1164–1176. doi: 10.1128/jb.92.4.1164-1176.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parker R. F., Davis J. K., Blalock D. K., Thorp R. B., Simecka J. W., Cassell G. H. Pulmonary clearance of Mycoplasma pulmonis in C57BL/6N and C3H/HeN mice. Infect Immun. 1987 Nov;55(11):2631–2635. doi: 10.1128/iai.55.11.2631-2635.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stadtländer C., Hartmann D., Binder A., Kirchhoff H. Investigation of seal mycoplasmas for their cytotoxic potential on tracheal organ cultures of SPF and gnotobiotic rats. Zentralbl Bakteriol. 1989 Dec;272(2):216–224. doi: 10.1016/s0934-8840(89)80010-6. [DOI] [PubMed] [Google Scholar]
  20. Stadtländer C., Kirchhoff H. Surface parasitism of the fish mycoplasma Mycoplasma mobile 163 K on tracheal epithelial cells. Vet Microbiol. 1990 Feb;21(4):339–343. doi: 10.1016/0378-1135(90)90005-g. [DOI] [PubMed] [Google Scholar]
  21. Stadtländer C., Kirchhoff H. The effects of Mycoplasma mobile 163 K on the ciliary epithelium of tracheal organ cultures. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;269(3):355–365. doi: 10.1016/s0176-6724(88)80179-2. [DOI] [PubMed] [Google Scholar]
  22. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Watson H. L., McDaniel L. S., Blalock D. K., Fallon M. T., Cassell G. H. Heterogeneity among strains and a high rate of variation within strains of a major surface antigen of Mycoplasma pulmonis. Infect Immun. 1988 May;56(5):1358–1363. doi: 10.1128/iai.56.5.1358-1363.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Westerberg S. C., Smith C. B., Wiley B. B., Jensen C. Mycoplasma-virus interrelationships in mouse tracheal organ cultures. Infect Immun. 1972 Jun;5(6):840–846. doi: 10.1128/iai.5.6.840-846.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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