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. 1991 Nov;59(11):4249–4251. doi: 10.1128/iai.59.11.4249-4251.1991

Characterization of murine monoclonal antibodies that recognize defined epitopes of pertussis toxin and neutralize its toxic effect on Chinese hamster ovary cells.

M J Walker 1, J Wehland 1, K N Timmis 1, B Raupach 1, M A Schmidt 1
PMCID: PMC259023  PMID: 1718872

Abstract

Three murine monoclonal antibodies (MAb), E19, E205, and E251, raised against pertussis toxin reacted in Western blots (immunoblots) with the S1, S4, and S2-S3 subunits, respectively, and neutralized the Chinese hamster ovary cell-clustering activity of pertussis toxin. MAb E251 recognized a linear synthetic peptide corresponding to amino acids 107 to 120 of the S2 subunit, suggesting a role for this region in receptor binding.

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Selected References

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