Skip to main content
PMC home page
The Yale Journal of Biology and Medicine logoLink to The Yale Journal of Biology and Medicine
. 1987 Mar-Apr;60(2):159–167.

Latent herpesvirus infections of neurons in guinea pigs and humans.

R B Tenser, R W Hyman
PMCID: PMC2590332  PMID: 3495074

Abstract

Latent herpes simplex virus (HSV) infection of the trigeminal ganglion of guinea pigs and latent varicella-zoster virus (VZV) infection of the trigeminal ganglion of humans were studied by in situ nucleic acid hybridization. Guinea pig trigeminal ganglia were removed during the period of viral latency (four to five weeks after corneal inoculation of HSV), and human ganglia were removed at autopsy. Radiolabeled HSV and VZV DNAs were used to probe ganglion tissue sections for viral-specified RNA. Hybridization detected only over neurons was present in 46 percent of ganglia from 22 latently infected guinea pigs and from 33 percent of ganglia from 10 human subjects. These results support the conclusion that some viral transcription occurred during HSV and VZV latency.

Full text

PDF
159

Images in this article

161FIG. 1
on p.161
162FIG. 2
on p.162
163FIG. 3
on p.163
163FIG. 4
on p.163
164FIG. 5
on p.164
165FIG. 6
on p.165

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brahic M., Haase A. T. Detection of viral sequences of low reiteration frequency by in situ hybridization. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6125–6129. doi: 10.1073/pnas.75.12.6125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cook M. L., Bastone V. B., Stevens J. G. Evidence that neurons harbor latent herpes simplex virus. Infect Immun. 1974 May;9(5):946–951. doi: 10.1128/iai.9.5.946-951.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Galloway D. A., Fenoglio C., Shevchuk M., McDougall J. K. Detection of herpes simplex RNA in human sensory ganglia. Virology. 1979 May;95(1):265–268. doi: 10.1016/0042-6822(79)90429-x. [DOI] [PubMed] [Google Scholar]
  4. Hall W. W., Choppin P. W. Measles-virus proteins in the brain tissue of patients with subacute sclerosing panencephalitis: absence of the M protein. N Engl J Med. 1981 May 7;304(19):1152–1155. doi: 10.1056/NEJM198105073041906. [DOI] [PubMed] [Google Scholar]
  5. Hyman R. W., Ecker J. R., Tenser R. B. Varicella-zoster virus RNA in human trigeminal ganglia. Lancet. 1983 Oct 8;2(8354):814–816. doi: 10.1016/s0140-6736(83)90736-5. [DOI] [PubMed] [Google Scholar]
  6. Jones T. R., Hyman R. W. Specious hybridization between herpes simplex virus DNA and human cellular DNA. Virology. 1983 Dec;131(2):555–560. doi: 10.1016/0042-6822(83)90521-4. [DOI] [PubMed] [Google Scholar]
  7. Jones T. R., Parks C. L., Spector D. J., Hyman R. W. Hybridization of herpes simplex virus DNA and human ribosomal DNA and RNA. Virology. 1985 Jul 30;144(2):384–397. doi: 10.1016/0042-6822(85)90280-6. [DOI] [PubMed] [Google Scholar]
  8. MELNICK J. L. Isolation of virus and the development of neutralizing and complement-fixing antibodies in poliomyelitic patients. Ann N Y Acad Sci. 1955 Sep 27;61(4):1005–1010. doi: 10.1111/j.1749-6632.1955.tb42559.x. [DOI] [PubMed] [Google Scholar]
  9. Maitland N. J., Kinross J. H., Busuttil A., Ludgate S. M., Smart G. E., Jones K. W. The detection of DNA tumour virus-specific RNA sequences in abnormal human cervical biopsies by in situ hybridization. J Gen Virol. 1981 Jul;55(Pt 1):123–137. doi: 10.1099/0022-1317-55-1-123. [DOI] [PubMed] [Google Scholar]
  10. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  11. Stroop W. G., Rock D. L., Fraser N. W. Localization of herpes simplex virus in the trigeminal and olfactory systems of the mouse central nervous system during acute and latent infections by in situ hybridization. Lab Invest. 1984 Jul;51(1):27–38. [PubMed] [Google Scholar]
  12. Tellez-Negal I., Harter D. H. Subacute sclerosing leukoencephalitis: ultrastructure of intranuclear and intracytoplasmic inclusions. Science. 1966 Nov 18;154(3751):899–901. doi: 10.1126/science.154.3751.899. [DOI] [PubMed] [Google Scholar]
  13. Tenser R. B., Dawson M., Ressel S. J., Dunstan M. E. Detection of herpes simplex virus mRNA in latently infected trigeminal ganglion neurons by in situ hybridization. Ann Neurol. 1982 Mar;11(3):285–291. doi: 10.1002/ana.410110309. [DOI] [PubMed] [Google Scholar]
  14. Tenser R. B. Herpes simplex and herpes zoster. Nervous system involvement. Neurol Clin. 1984 May;2(2):215–240. [PubMed] [Google Scholar]
  15. Tenser R. B., Sommerville K. W., Mummaw J. G., Frisque R. J. Isolation of JC virus capsomer-like structures from progressive multifocal leukoencephalopathy brain. J Neurol Sci. 1986 Feb;72(2-3):243–254. doi: 10.1016/0022-510x(86)90012-2. [DOI] [PubMed] [Google Scholar]
  16. Weiner L. P., Narayan O., Penney J. B., Jr, Herndon R. M., Feringa E. R., Tourtellotte W. W., Johnson R. T. Papovavirus of JC type in progressive multifocal leukoencephalopathy. Rapid identification and subsequent isolation. Arch Neurol. 1973 Jul;29(1):1–3. doi: 10.1001/archneur.1973.00490250019001. [DOI] [PubMed] [Google Scholar]

Articles from The Yale Journal of Biology and Medicine are provided here courtesy of Yale Journal of Biology and Medicine

RESOURCES