Abstract
The partial characterization of the structure of the lipoglycan (LG) from Acholeplasma axanthum is added to the previous complete structural analysis of the lipoglycan from A. granularum. The terminal sequence of A. axanthum LG is Glcp(beta 1----2)-Glcp(beta 1----2)-Glcp(beta 1----6)-; of A. granularum Glcp(beta 1----2)-Glcp(alpha 1----4)-Glcp(beta 1----4)-. These specific residues define the major antigenic determinants of the LG as determined by blockage of hemagglutination of LG coated erythrocytes by specific oligosaccharides and binding of radiolabeled LG to specific immunoglobulins. The binding of LG to mammalian cells occurs by an interaction between specific eucaryotic cell receptors and the internal sequence of the oligosaccharide chain of LG. Size and sugar chains of LG rather than fatty acid residues appears to define the binding site on the LG.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Al-Samarrai T. H., Smith P. F., Lynn R. J. Identification of the major antigenic determinants on lipoglycans from Acholeplasma granularum and Acholeplasma axanthum. Infect Immun. 1983 May;40(2):629–632. doi: 10.1128/iai.40.2.629-632.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Al-Shammari A. J., Smith P. F. Interaction of mycoplasma virus type 2 with cellular components of Acholeplasma laidlawii strain JA1. J Virol. 1980 Oct;36(1):120–124. doi: 10.1128/jvi.36.1.120-124.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lynn R. J., Guidici P. A., Hollemweguer E. J., Smith P. F. Humoral immune response of rabbits to acholeplasmal lipoglycans. Infect Immun. 1980 Sep;29(3):926–933. doi: 10.1128/iai.29.3.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seid R. C., Jr, Smith P. F., Guevarra G., Hochstein H. D., Barile M. F. Endotoxin-like activities of mycoplasmal lipopolysaccharides (lipoglycans). Infect Immun. 1980 Sep;29(3):990–994. doi: 10.1128/iai.29.3.990-994.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. F. Homogeneity of lipopolysaccharides from Acholeplasma. J Bacteriol. 1977 Apr;130(1):393–398. doi: 10.1128/jb.130.1.393-398.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. F., Langworthy T. A., Mayberry W. R. Distribution and composition of lipopolysaccharides from mycoplasmas. J Bacteriol. 1976 Mar;125(3):916–922. doi: 10.1128/jb.125.3.916-922.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. F. Structure of the oligosaccharide chain of lipoglycan from Acholeplasma granularum. Biochim Biophys Acta. 1981 Jul 24;665(1):92–99. doi: 10.1016/0005-2760(81)90237-x. [DOI] [PubMed] [Google Scholar]
- Stupp Y., Yoshida T., Paul W. E. Determination of antibody-hapten equilibrium constants by an ammonium sulfate precipitation technique. J Immunol. 1969 Sep;103(3):625–627. [PubMed] [Google Scholar]