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. 1991 Dec;59(12):4377–4382. doi: 10.1128/iai.59.12.4377-4382.1991

Effect of elevated environmental temperature on the antibody response of mice to Trypanosoma cruzi during the acute phase of infection.

K A Dimock 1, C D Davis 1, R E Kuhn 1
PMCID: PMC259052  PMID: 1937796

Abstract

When held at 36 degrees C, Trypanosoma cruzi-infected C3H mice survive an otherwise lethal infection with significantly decreased parasitemia levels and enhanced immune responsiveness. Treatment of T. cruzi-infected mice with the immunosuppressive agent cyclophosphamide indicated that the positive effects of increased environmental temperature were primarily due to enhancement of immunity. A parasite-specific, enzyme-linked immunosorbent assay and immunoblot analysis were used to examine the effect of elevated environmental temperature on the production of anti-T. cruzi antibodies. Both the reactivity and diversity of anti-T. cruzi antibodies were found to be lower in infected mice held at 36 degrees C than in infected mice held at room temperature. However, reactivity and diversity could be enhanced by vaccination with culture forms of the parasite.

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Selected References

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  1. Amrein Y. U. Effects of environmental temperature on Trypanosoma cruzi infection in mice. J Parasitol. 1967 Dec;53(6):1160–1160. [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Carvalho J. F., de Carvalho E. F., Rondinelli E., Silva R., de Castro F. T. Protein biosynthesis changes in Trypanosoma cruzi induced by supra-optimal temperature. Exp Cell Res. 1987 Feb;168(2):338–346. doi: 10.1016/0014-4827(87)90006-1. [DOI] [PubMed] [Google Scholar]
  4. Choromanski L., Kuhn R. E. Interleukin 2 enhances specific and nonspecific immune responses in experimental Chagas' disease. Infect Immun. 1985 Nov;50(2):354–357. doi: 10.1128/iai.50.2.354-357.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Choromanski L., Kuhn R. E. Repeated antigenic stimulation overcomes immunosuppression in experimental Chagas' disease. Immunology. 1986 Oct;59(2):289–294. [PMC free article] [PubMed] [Google Scholar]
  6. Cunningham D. S., Kuhn R. E. Trypanosoma cruzi-induced suppression of the primary immune response in murine cell cultures to T-cell-dependent and -independent antigens. J Parasitol. 1980 Feb;66(1):16–27. [PubMed] [Google Scholar]
  7. Davis C. D., Kuhn R. E. Selective binding of Trypanosoma cruzi to host cell membrane polypeptides. Infect Immun. 1990 Jan;58(1):1–6. doi: 10.1128/iai.58.1.1-6.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duff G. W., Durum S. K. The pyrogenic and mitogenic actions of interleukin-1 are related. Nature. 1983 Aug 4;304(5925):449–451. doi: 10.1038/304449a0. [DOI] [PubMed] [Google Scholar]
  9. Engman D. M., Dragon E. A., Donelson J. E. Human humoral immunity to hsp70 during Trypanosoma cruzi infection. J Immunol. 1990 May 15;144(10):3987–3991. [PubMed] [Google Scholar]
  10. Estrada J. J., Kuhn R. E. Immunochemical detection of antigens of larval Taenia solium and anti-larval antibodies in the cerebrospinal fluid of patients with neurocysticercosis. J Neurol Sci. 1985 Nov;71(1):39–48. doi: 10.1016/0022-510x(85)90035-8. [DOI] [PubMed] [Google Scholar]
  11. Guhl F., Marinkelle C. J., de Sánchez N., Grögl M. Estudios sobre la transferencia de inmunidad humoral contra Trypanosoma cruzi inducida por calor en ratones. Rev Inst Med Trop Sao Paulo. 1979 Jul-Aug;21(4):166–171. [PubMed] [Google Scholar]
  12. Jampel H. D., Duff G. W., Gershon R. K., Atkins E., Durum S. K. Fever and immunoregulation. III. Hyperthermia augments the primary in vitro humoral immune response. J Exp Med. 1983 Apr 1;157(4):1229–1238. doi: 10.1084/jem.157.4.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kuhn R. E., Murnane J. E. Trypanosoma cruzi: immune destruction of parasitized mouse fibroblasts in vitro. Exp Parasitol. 1977 Feb;41(1):66–73. doi: 10.1016/0014-4894(77)90130-8. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Marinkelle C. J., Rodriguez E. The influence of environmental temperature on the pathogenicity of Trypanosoma cruzi in mice. Exp Parasitol. 1968 Oct;23(2):260–263. doi: 10.1016/0014-4894(68)90067-2. [DOI] [PubMed] [Google Scholar]
  16. Otieno L. H. Effects of immunosuppressive agents on the course of Trypanosoma (trypanozoon) brucei infections in heat-stressed mice. Trans R Soc Trop Med Hyg. 1973;67(6):856–869. doi: 10.1016/0035-9203(73)90015-1. [DOI] [PubMed] [Google Scholar]
  17. Otieno L. H. Influence of ambient temperature on the course of experimental trypanosomiasis in mice. Ann Trop Med Parasitol. 1972 Mar;66(1):15–24. doi: 10.1080/00034983.1972.11686793. [DOI] [PubMed] [Google Scholar]
  18. Powell M. R., Kuhn R. E. Measurement of cytolytic antibody in experimental Chagas' disease using a terminal radiolabelling procedure. J Parasitol. 1980 Jun;66(3):399–406. [PubMed] [Google Scholar]
  19. Pung O. J., Hulsebos L. H., Kuhn R. E. Experimental American leishmaniasis and Chagas' disease in the Brazilian squirrel monkey: cross immunity and electrocardiographic studies of monkeys infected with Leishmania braziliensis and Trypanosoma cruzi. Int J Parasitol. 1988 Dec;18(8):1053–1059. doi: 10.1016/0020-7519(88)90075-6. [DOI] [PubMed] [Google Scholar]
  20. Saririan K., Nickerson D. A. Enhancement of murine in vitro antibody formation by hyperthermia. Cell Immunol. 1982 Dec;74(2):306–312. doi: 10.1016/0008-8749(82)90031-4. [DOI] [PubMed] [Google Scholar]
  21. Smith C. J., Levine R. F., Mansfield J. M. Cloning of African trypanosomes in mice immunosuppressed by cyclophosphamide treatment. Am J Trop Med Hyg. 1982 Nov;31(6):1098–1102. doi: 10.4269/ajtmh.1982.31.1098. [DOI] [PubMed] [Google Scholar]
  22. Smith J. B., Knowlton R. P., Agarwal S. S. Human lymphocyte responses are enhanced by culture at 40 degrees C. J Immunol. 1978 Aug;121(2):691–694. [PubMed] [Google Scholar]
  23. TREJOS A., GODOY G. A., GREENBLATT G., CEDILLOS R. Effects of temperature on morphologic variation of Schizotrypanum cruzi in tissue culture. Exp Parasitol. 1963 Apr;13:211–218. doi: 10.1016/0014-4894(63)90073-0. [DOI] [PubMed] [Google Scholar]
  24. Tarleton R. L., Kuhn R. E. Changes in cell populations and immunoglobulin-producing cells in the spleens of mice infected with Trypanosoma cruzi: correlations with parasite-specific antibody response. Cell Immunol. 1983 Sep;80(2):392–404. doi: 10.1016/0008-8749(83)90126-0. [DOI] [PubMed] [Google Scholar]
  25. Tarleton R. L., Kuhn R. E. Measurement of parasite-specific immune responses in vitro: evidence for suppression of the antibody response to Trypanosoma cruzi. Eur J Immunol. 1985 Aug;15(8):845–850. doi: 10.1002/eji.1830150820. [DOI] [PubMed] [Google Scholar]
  26. Tarleton R. L., Kuhn R. E. Restoration of in vitro immune responses of spleen cells from mice infected with Trypanosoma cruzi by supernatants containing interleukin 2. J Immunol. 1984 Sep;133(3):1570–1575. [PubMed] [Google Scholar]

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